PMID- 20566350
OWN - NLM
STAT- MEDLINE
DCOM- 20101103
LR  - 20230425
IS  - 1879-0542 (Electronic)
IS  - 0165-2478 (Print)
IS  - 0165-2478 (Linking)
VI  - 132
IP  - 1-2
DP  - 2010 Aug 16
TI  - Efficient generation of a monoclonal antibody against the human C-type lectin 
      receptor DCIR by targeting murine dendritic cells.
PG  - 69-78
LID - 10.1016/j.imlet.2010.06.002 [doi]
AB  - Dendritic cells (DCs) are very important for the generation of long lasting 
      immune responses against pathogens or the induction of anti-tumor responses. 
      Targeting antigen to dendritic cells via monoclonal antibodies specific for DC 
      cell surface receptors such as DEC205 was shown to elicit potent cellular and 
      humoral immune responses in vivo. Therefore, we investigated whether this novel 
      strategy might also be useful for the generation of new monoclonal antibodies 
      against molecules of choice. We show, that by targeting the extracellular domain 
      of the human C-type lectin receptor ClecSF6/DCIR/LLIR (hDCIR) to DEC205 on DCs in 
      vivo, we were able to generate highly specific monoclonal antibodies against 
      hDCIR.
CI  - Copyright 2010 Elsevier B.V. All rights reserved.
FAU - Heidkamp, Gordon F
AU  - Heidkamp GF
AD  - Laboratory of Dendritic Cell Biology, Nikolaus-Fiebiger-Center of Molecular 
      Medicine and Department of Dermatology, University Hospital of Erlangen, 
      Glueckstr. 6, 91054 Erlangen, Germany.
FAU - Neubert, Kirsten
AU  - Neubert K
FAU - Haertel, Eric
AU  - Haertel E
FAU - Nimmerjahn, Falk
AU  - Nimmerjahn F
FAU - Nussenzweig, Michel C
AU  - Nussenzweig MC
FAU - Dudziak, Diana
AU  - Dudziak D
LA  - eng
GR  - HHMI/Howard Hughes Medical Institute/United States
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100608
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (CD11c Antigen)
RN  - 0 (CLEC4A protein, human)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, Immunologic)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/*biosynthesis/immunology
MH  - Antigen Presentation/immunology
MH  - CD11c Antigen/metabolism
MH  - Cell Differentiation/immunology
MH  - Dendritic Cells/cytology/*immunology/metabolism
MH  - Female
MH  - Humans
MH  - Lectins, C-Type/chemistry/*immunology/metabolism
MH  - Leukocytes, Mononuclear/immunology/metabolism
MH  - Membrane Glycoproteins/chemistry/*immunology/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Receptors, Immunologic/chemistry/*immunology/metabolism
PMC - PMC2997960
MID - NIHMS226957
OID - NLM: HHMIMS226957
COIS- The other authors have no conflicting financial interests.
EDAT- 2010/06/23 06:00
MHDA- 2010/11/04 06:00
PMCR- 2011/02/01
CRDT- 2010/06/23 06:00
PHST- 2009/11/23 00:00 [received]
PHST- 2010/05/25 00:00 [revised]
PHST- 2010/06/01 00:00 [accepted]
PHST- 2010/06/23 06:00 [entrez]
PHST- 2010/06/23 06:00 [pubmed]
PHST- 2010/11/04 06:00 [medline]
PHST- 2011/02/01 00:00 [pmc-release]
AID - S0165-2478(10)00166-5 [pii]
AID - 10.1016/j.imlet.2010.06.002 [doi]
PST - ppublish
SO  - Immunol Lett. 2010 Aug 16;132(1-2):69-78. doi: 10.1016/j.imlet.2010.06.002. Epub 
      2010 Jun 8.