PMID- 20566715
OWN - NLM
STAT- MEDLINE
DCOM- 20100730
LR  - 20240109
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Print)
IS  - 0022-1007 (Linking)
VI  - 207
IP  - 7
DP  - 2010 Jul 5
TI  - Allelic polymorphism in the T cell receptor and its impact on immune responses.
PG  - 1555-67
LID - 10.1084/jem.20100603 [doi]
AB  - In comparison to human leukocyte antigen (HLA) polymorphism, the impact of 
      allelic sequence variation within T cell receptor (TCR) loci is much less 
      understood. Particular TCR loci have been associated with autoimmunity, but the 
      molecular basis for this phenomenon is undefined. We examined the T cell response 
      to an HLA-B*3501-restricted epitope (HPVGEADYFEY) from Epstein-Barr virus (EBV), 
      which is frequently dominated by a TRBV9*01(+) public TCR (TK3). However, the 
      common allelic variant TRBV9*02, which differs by a single amino acid near the 
      CDR2beta loop (Gln55-->His55), was never used in this response. The structure of 
      the TK3 TCR, its allelic variant, and a nonnaturally occurring mutant 
      (Gln55-->Ala55) in complex with HLA-B*3501(HPVGEADYFEY) revealed that the 
      Gln55-->His55 polymorphism affected the charge complementarity at the 
      TCR-peptide-MHC interface, resulting in reduced functional recognition of the 
      cognate and naturally occurring variants of this EBV peptide. Thus, polymorphism 
      in the TCR loci may contribute toward variability in immune responses and the 
      outcome of infection.
FAU - Gras, Stephanie
AU  - Gras S
AD  - The Protein Crystallography Unit, Department of Biochemistry and Molecular 
      Biology, Monash University, Clayton, Victoria 3800, Australia.
FAU - Chen, Zhenjun
AU  - Chen Z
FAU - Miles, John J
AU  - Miles JJ
FAU - Liu, Yu Chih
AU  - Liu YC
FAU - Bell, Melissa J
AU  - Bell MJ
FAU - Sullivan, Lucy C
AU  - Sullivan LC
FAU - Kjer-Nielsen, Lars
AU  - Kjer-Nielsen L
FAU - Brennan, Rebekah M
AU  - Brennan RM
FAU - Burrows, Jacqueline M
AU  - Burrows JM
FAU - Neller, Michelle A
AU  - Neller MA
FAU - Khanna, Rajiv
AU  - Khanna R
FAU - Purcell, Anthony W
AU  - Purcell AW
FAU - Brooks, Andrew G
AU  - Brooks AG
FAU - McCluskey, James
AU  - McCluskey J
FAU - Rossjohn, Jamie
AU  - Rossjohn J
FAU - Burrows, Scott R
AU  - Burrows SR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100621
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Epitopes)
RN  - 0 (HLA-B Antigens)
RN  - 0 (HLA-B*35:01 antigen)
RN  - 0 (HLA-B35 Antigen)
RN  - 0 (Peptides)
RN  - 0 (Receptors, Antigen, T-Cell)
SB  - IM
MH  - *Alleles
MH  - Amino Acid Sequence
MH  - Cell Line, Tumor
MH  - Epitopes/chemistry/genetics
MH  - HLA-B Antigens/chemistry/genetics
MH  - HLA-B35 Antigen
MH  - Humans
MH  - Immunity/*genetics
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Mutation/genetics
MH  - Peptides/chemistry/immunology
MH  - Polymorphism, Single Nucleotide/*genetics
MH  - Protein Structure, Secondary
MH  - Receptors, Antigen, T-Cell/chemistry/*genetics
MH  - Surface Plasmon Resonance
PMC - PMC2901058
EDAT- 2010/06/23 06:00
MHDA- 2010/07/31 06:00
PMCR- 2011/01/05
CRDT- 2010/06/23 06:00
PHST- 2010/06/23 06:00 [entrez]
PHST- 2010/06/23 06:00 [pubmed]
PHST- 2010/07/31 06:00 [medline]
PHST- 2011/01/05 00:00 [pmc-release]
AID - jem.20100603 [pii]
AID - 20100603 [pii]
AID - 10.1084/jem.20100603 [doi]
PST - ppublish
SO  - J Exp Med. 2010 Jul 5;207(7):1555-67. doi: 10.1084/jem.20100603. Epub 2010 Jun 
      21.