PMID- 20566900
OWN - NLM
STAT- MEDLINE
DCOM- 20101029
LR  - 20210206
IS  - 1528-0020 (Electronic)
IS  - 0006-4971 (Linking)
VI  - 116
IP  - 11
DP  - 2010 Sep 16
TI  - Drug-induced thrombocytopenia: development of a novel NOD/SCID mouse model to 
      evaluate clearance of circulating platelets by drug-dependent antibodies and the 
      efficacy of IVIG.
PG  - 1958-60
LID - 10.1182/blood-2010-02-268326 [doi]
AB  - Drug-induced immune thrombocytopenia (DITP) is an adverse drug effect mediated by 
      drug-dependent antibodies. Intravenous immunoglobulin (IVIG) is frequently used 
      to treat DITP and primary immune thrombocytopenia (ITP). Despite IVIG's proven 
      beneficial effects in ITP, its efficacy in DITP is unclear. We have established a 
      nonobese diabetic/severe combined immunodeficient (NOD/SCID) mouse model of DITP 
      in which human platelets survive for more than 24 hours, allowing platelet 
      clearance by DITP/ITP antibodies to be studied. Rapid human platelet clearance 
      was uniformly observed with all quinine-induced thrombocytopenia (QITP) patient 
      sera studied (mean platelet lifespans: QITP 1.5 +/- 0.3 hours vs controls 16.5 +/- 
      4.3 hours), consistent with the clinical presentation of DITP. In contrast, 
      clearance rates with ITP antibodies were more variable. IVIG treatment partially 
      prevented platelet clearance by DITP and ITP antibodies. Our results suggest that 
      the NOD/SCID mouse model is useful for investigating the efficacy of current and 
      future DITP therapies, an area in which there is little experimental evidence to 
      guide treatment.
FAU - Liang, Simon X
AU  - Liang SX
AD  - St George Clinical School, University of New South Wales, Sydney, Australia.
FAU - Pinkevych, Mykola
AU  - Pinkevych M
FAU - Khachigian, Levon M
AU  - Khachigian LM
FAU - Parish, Christopher R
AU  - Parish CR
FAU - Davenport, Miles P
AU  - Davenport MP
FAU - Chong, Beng H
AU  - Chong BH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100621
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antibodies)
RN  - 0 (Immunoglobulins, Intravenous)
RN  - A7V27PHC7A (Quinine)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Animals
MH  - Antibodies/immunology/metabolism/pharmacology
MH  - Antibody Specificity/immunology
MH  - Blood Platelets/cytology/immunology/*metabolism
MH  - Cell Survival/drug effects
MH  - *Disease Models, Animal
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Immunoglobulins, Intravenous/administration & dosage/*pharmacology
MH  - Male
MH  - Mice
MH  - Mice, Inbred NOD
MH  - Mice, SCID
MH  - Middle Aged
MH  - Quinine/administration & dosage/adverse effects
MH  - Thrombocytopenia/blood/chemically induced/*drug therapy
MH  - Time Factors
MH  - Treatment Outcome
MH  - Young Adult
EDAT- 2010/06/23 06:00
MHDA- 2010/10/30 06:00
CRDT- 2010/06/23 06:00
PHST- 2010/06/23 06:00 [entrez]
PHST- 2010/06/23 06:00 [pubmed]
PHST- 2010/10/30 06:00 [medline]
AID - S0006-4971(20)33000-7 [pii]
AID - 10.1182/blood-2010-02-268326 [doi]
PST - ppublish
SO  - Blood. 2010 Sep 16;116(11):1958-60. doi: 10.1182/blood-2010-02-268326. Epub 2010 
      Jun 21.