PMID- 20567845
OWN - NLM
STAT- MEDLINE
DCOM- 20100928
LR  - 20211020
IS  - 1432-2307 (Electronic)
IS  - 0945-6317 (Linking)
VI  - 457
IP  - 3
DP  - 2010 Sep
TI  - Strength of molecular cytogenetic analyses for adjusting the diagnosis of renal 
      cell carcinomas with both clear cells and papillary features: a study of three 
      cases.
PG  - 397-404
LID - 10.1007/s00428-010-0937-1 [doi]
AB  - Histological features are usually sufficient for providing an accurate diagnosis 
      of renal cell carcinomas (RCC). However, the morphological appearance might 
      sometimes be misleading. For instance, RCC with papillary areas and extensive 
      clear cell changes may be difficult to classify either as clear cell renal 
      carcinoma or as papillary renal cell carcinoma (pRCC). We used the combination of 
      immunohistochemistry, conventional cytogenetics, fluorescence in situ 
      hybridization (FISH), bacterial artificial chromosomes comparative genomic 
      hybridization arrays and high-density single nucleotides polymorphism arrays (SNP 
      arrays) to characterize three cases of RCC showing a predominant cytology of 
      cells with clear cytoplasm and variable amounts of papillary areas. In accordance 
      with the 2004 World Health Organization (WHO) classification, we initially 
      assessed the diagnosis of clear cell RCC for one of the cases and unclassified 
      RCC for the two remaining cases. However, because of a strong immunohistochemical 
      labeling for alpha-methylacyl-CoA racemase, as well as the presence of a gain of 
      chromosomes 7 and 17, we concluded that two of these tumors were actually pRCC. 
      As for the third case, because of the presence of both pCCR and ccCCR molecular 
      cytogenetic aberrations, including gains of chromosomes 7 and 17, loss of 
      chromosome Y and whole chromosome 3 loss of heterozyosity (isodisomy), the final 
      diagnosis was hybrid tumor cc-pRCC, so-called "unclassified RCC" according to the 
      WHO classification. Our observations demonstrate the necessity to use 
      immunohistochemical and cytogenetic tools in all cases of RCC showing unusual 
      features. The combination of FISH and SNP arrays is prevailing for characterizing 
      cases with hybrid features.
FAU - Haudebourg, Juliette
AU  - Haudebourg J
AD  - Department of Pathology, Nice University Hospital, Nice, France. 
      juliette.haudebourg@nice.fnclcc.fr
FAU - Hoch, Benjamin
AU  - Hoch B
FAU - Fabas, Thibault
AU  - Fabas T
FAU - Cardot-Leccia, Nathalie
AU  - Cardot-Leccia N
FAU - Burel-Vandenbos, Fanny
AU  - Burel-Vandenbos F
FAU - Vieillefond, Annick
AU  - Vieillefond A
FAU - Amiel, Jean
AU  - Amiel J
FAU - Michiels, Jean-Francois
AU  - Michiels JF
FAU - Pedeutour, Florence
AU  - Pedeutour F
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100622
PL  - Germany
TA  - Virchows Arch
JT  - Virchows Archiv : an international journal of pathology
JID - 9423843
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carcinoma, Renal Cell/*diagnosis/genetics
MH  - Chromosomes, Artificial, Bacterial
MH  - Comparative Genomic Hybridization
MH  - *Cytogenetic Analysis
MH  - Female
MH  - Humans
MH  - Immunohistochemistry/*methods
MH  - In Situ Hybridization, Fluorescence
MH  - Kidney Neoplasms/*diagnosis/genetics
MH  - Male
MH  - Middle Aged
MH  - Oligonucleotide Array Sequence Analysis
MH  - Polymorphism, Single Nucleotide
EDAT- 2010/06/23 06:00
MHDA- 2010/09/30 06:00
CRDT- 2010/06/23 06:00
PHST- 2010/02/01 00:00 [received]
PHST- 2010/05/18 00:00 [accepted]
PHST- 2010/05/07 00:00 [revised]
PHST- 2010/06/23 06:00 [entrez]
PHST- 2010/06/23 06:00 [pubmed]
PHST- 2010/09/30 06:00 [medline]
AID - 10.1007/s00428-010-0937-1 [doi]
PST - ppublish
SO  - Virchows Arch. 2010 Sep;457(3):397-404. doi: 10.1007/s00428-010-0937-1. Epub 2010 
      Jun 22.