PMID- 20568461
OWN - NLM
STAT- MEDLINE
DCOM- 20120706
LR  - 20211203
IS  - 1000-8020 (Print)
IS  - 1000-8020 (Linking)
VI  - 39
IP  - 3
DP  - 2010 May
TI  - [Comparison of immunosuppression induced by different doses of cyclophosphamide 
      in normal mice].
PG  - 313-5, 325
AB  - OBJECTIVE: To compare the methods for developing immune-suppressed mice models 
      induced by cyclophosphamide (CTX) with different dosages and ways. METHODS: The 
      mice were assigned to three groups; distilled water were given by gavage daily to 
      the mice in control group; 60 mg/kg of CTX were given by gavage daily to the mice 
      in CTX-1 group; and the mice in CTX-2 group were intraperitoneally injected (ip) 
      with 200 mg/kg bw of CTX one day before the end of the experiment. The experiment 
      lasted for 30 days. RESULTS: The body weight, spleen weight, the percentage of 
      lymphocytes in peripheral blood, activities of natural killer cells (NK) and 
      cytotoxic T lymphocytes (CTL) and HC50 in CTX-1 group were significantly lower 
      than those in the control group (P < 0.05). The percentage of T lymphocytes in 
      peripheral blood, the percentage of Th and Ts lymphocytes and Th/Ts ratio in 
      CTX-2 group were significantly higher than those in the control group (P < 0.05). 
      Other immunological indices in CTX-2 group were significantly lower than the 
      control group (P < 0.05). CONCLUSION: A proper immunosuppressed animal model for 
      the functional food evaluating system could be established by gavaging 60 mg/kg 
      bw of CTX for 30 days or by one intraperitoneal injection (ip) of 200 mg/kg. But 
      the former might be more fitted to establish the immunosuppressed model.
FAU - Qi, Lijuan
AU  - Qi L
AD  - Institute for Nutrition and Food Safty, China CDC, Beijing 100021, China.
FAU - Song, Yan
AU  - Song Y
FAU - Wang, Wei
AU  - Wang W
FAU - Cui, Wenming
AU  - Cui W
FAU - Zhang, Xin
AU  - Zhang X
FAU - Liu, Zhaoping
AU  - Liu Z
FAU - Sun, Nana
AU  - Sun N
FAU - Li, Ning
AU  - Li N
LA  - chi
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Wei Sheng Yan Jiu
JT  - Wei sheng yan jiu = Journal of hygiene research
JID - 9426367
RN  - 0 (Immunosuppressive Agents)
RN  - 8N3DW7272P (Cyclophosphamide)
SB  - IM
MH  - Animals
MH  - Cyclophosphamide/*administration & dosage
MH  - *Disease Models, Animal
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Immunocompromised Host
MH  - *Immunosuppression Therapy
MH  - Immunosuppressive Agents/*administration & dosage
MH  - Mice
MH  - Mice, Inbred BALB C
EDAT- 2010/06/24 06:00
MHDA- 2012/07/07 06:00
CRDT- 2010/06/24 06:00
PHST- 2010/06/24 06:00 [entrez]
PHST- 2010/06/24 06:00 [pubmed]
PHST- 2012/07/07 06:00 [medline]
PST - ppublish
SO  - Wei Sheng Yan Jiu. 2010 May;39(3):313-5, 325.