PMID- 20570063
OWN - NLM
STAT- MEDLINE
DCOM- 20110504
LR  - 20181201
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 146
IP  - 3-4
DP  - 2010 Dec 15
TI  - The immunogenicity of DNA constructs co-expressing GP5 and M proteins of porcine 
      reproductive and respiratory syndrome virus conjugated by GPGP linker in pigs.
PG  - 189-99
LID - 10.1016/j.vetmic.2010.05.007 [doi]
AB  - The heterodimer of glycoprotein 5 (GP5) and non-glycosylated matrix protein (M) 
      is the leading target for the development of new generation of vaccines against 
      porcine reproductive and respiratory syndrome virus (PRRSV) infection. It has 
      been demonstrated that DNA vaccine co-expressing GP5 and M proteins as a fusion 
      protein aroused better immunogenicity than that expressing GP5 or M alone, but it 
      was no better than the DNA vaccine co-expressing GP5 and M proteins with two 
      different promoters. Altered natural conformation of the co-expressed GP5 and M 
      fusion protein was considered as the major cause. Glycine-proline-glycine-proline 
      (GPGP) linker can minimize the conformational changes in tertiary structure and 
      provide flexibility of the peptide chain. The objective of this study was to 
      evaluate whether the immunogenicity of DNA constructs co-expressing GP5 and M 
      proteins linked by GPGP could be enhanced in pigs. Three recombinant DNA 
      constructs expressing GP5/M fusion protein without GPGP linker (pcDNA-56), GP5/M 
      fusion protein conjugated by GPGP linker (pcDNA-5L6), and M/GP5 fusion protein 
      conjugated by GPGP linker (pcDNA-6L5) were established. Sixteen PRRSV-free pigs 
      were randomly assigned to four groups and inoculated intramuscularly with 3 
      consecutive doses of 500 mug of empty vector pcDNA3.1, pcDNA-56, pcDNA-5L6 or 
      pcDNA-6L5 each at a 2-week interval followed by challenge with 5 x 10(5) TCID(50) 
      PRRSV at 3 weeks after the final inoculation. All pcDNA-56-, pcDNA-5L6-, and 
      pcDNA-6L5- but not pcDNA-3.1-inoculated pigs developed neutralizing antibodies 
      (NAs) 3 weeks after the final inoculation and a gradual increase in NA titers 
      after PRRSV challenge, indicating that pigs inoculated with these DNA constructs 
      could establish a sufficient immune memory. The pcDNA-5L6- and 
      pcDNA-6L5-inoculated pigs displayed lower level and shorter period of viremia and 
      lower tissue viral load following PRRSV challenge than did the 
      pcDNA-56-inoculated pigs. The strategy of co-expressing GPGP-linked GP5 and M 
      fusion protein may be a promising approach for future PRRSV vaccine development, 
      possibly via the improvement of natural conformation of the target fusion 
      protein.
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Chia, Min-Yuan
AU  - Chia MY
AD  - Graduate Institute of Veterinary Medicine, School of Veterinary Medicine, Taipei 
      106, Taiwan, ROC.
FAU - Hsiao, Shih-Hsuan
AU  - Hsiao SH
FAU - Chan, Hui-Ting
AU  - Chan HT
FAU - Do, Yi-Yin
AU  - Do YY
FAU - Huang, Pung-Ling
AU  - Huang PL
FAU - Chang, Hui-Wen
AU  - Chang HW
FAU - Tsai, Yi-Chieh
AU  - Tsai YC
FAU - Lin, Chun-Ming
AU  - Lin CM
FAU - Pang, Victor Fei
AU  - Pang VF
FAU - Jeng, Chian-Ren
AU  - Jeng CR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100510
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antibodies, Viral)
RN  - 0 (M protein, porcine reproductive and respiratory syndrome virus)
RN  - 0 (Vaccines, DNA)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Matrix Proteins)
RN  - 0 (Viral Vaccines)
RN  - 0 (glycoprotein 5, PRRSV)
RN  - 17332-09-1 (GpGp)
RN  - 85-32-5 (Guanosine Monophosphate)
SB  - IM
MH  - Animals
MH  - Antibodies, Neutralizing/blood
MH  - Antibodies, Viral/blood
MH  - Guanosine Monophosphate/*analogs & derivatives/genetics
MH  - Plasmids/genetics
MH  - Porcine Reproductive and Respiratory Syndrome/immunology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*immunology
MH  - Swine
MH  - Vaccines, DNA/genetics/immunology
MH  - Viral Envelope Proteins/*genetics/*immunology
MH  - Viral Load
MH  - Viral Matrix Proteins/*genetics/*immunology
MH  - Viral Vaccines/genetics/*immunology
MH  - Viremia/immunology
EDAT- 2010/06/24 06:00
MHDA- 2011/05/05 06:00
CRDT- 2010/06/24 06:00
PHST- 2009/12/18 00:00 [received]
PHST- 2010/04/26 00:00 [revised]
PHST- 2010/05/03 00:00 [accepted]
PHST- 2010/06/24 06:00 [entrez]
PHST- 2010/06/24 06:00 [pubmed]
PHST- 2011/05/05 06:00 [medline]
AID - S0378-1135(10)00234-8 [pii]
AID - 10.1016/j.vetmic.2010.05.007 [doi]
PST - ppublish
SO  - Vet Microbiol. 2010 Dec 15;146(3-4):189-99. doi: 10.1016/j.vetmic.2010.05.007. 
      Epub 2010 May 10.