PMID- 20570135
OWN - NLM
STAT- MEDLINE
DCOM- 20101129
LR  - 20220321
IS  - 1879-0852 (Electronic)
IS  - 0959-8049 (Linking)
VI  - 46
IP  - 11
DP  - 2010 Jul
TI  - Prediction of liver metastasis after colorectal cancer using reverse 
      transcription-polymerase chain reaction analysis of 10 genes.
PG  - 2119-26
LID - 10.1016/j.ejca.2010.04.019 [doi]
AB  - PURPOSE: Liver metastasis is one of the major types of recurrence after surgery 
      for colorectal cancer. Traditional methods of predicting liver metastasis are 
      limited in their accuracy, suggesting the need to develop new predictors. We 
      developed a 10-gene signature that is closely associated with the development of 
      liver metastasis after colorectal cancer. PATIENTS AND METHODS: We examined a 
      total of 189 frozen specimens of primary colorectal cancers using both microarray 
      and quantitative real-time reverse transcriptase-polymerase chain reaction 
      (RT-PCR) analysis. Initially, we studied gene expression in colorectal cancer 
      tissue from 160 randomly selected patients who had undergone surgical resection 
      of colorectal cancer and evaluated the association between the level of gene 
      expression and the occurrence of liver metastasis. We developed a gene-expression 
      model for the prediction of liver metastasis based on the RT-PCR findings. We 
      then used specimens from 29 other patients for validation. RESULTS: The 
      expression of 14 genes was correlated with liver metastasis according to both 
      microarray and RT-PCR analysis. We constructed an accurate predictive model based 
      on the results for 10 of these genes, which included epiregulin (EREG), 
      amphiregulin (AREG), cyclooxygenase 2 (COX-2) and lymphocyte-specific protein 
      tyrosine kinase (LCK). The 10-gene signature was an independent predictor of 
      liver metastasis. The model was validated in the independent set of 29 patients. 
      The predictive accuracy of the model in a test set of patients was 86.2%. 
      CONCLUSION: The 10-gene signature identified in this study is closely associated 
      with the occurrence of liver metastasis in colorectal cancer patients.
CI  - Copyright 2010 Elsevier Ltd. All rights reserved.
FAU - Watanabe, Toshiaki
AU  - Watanabe T
AD  - Department of Surgery, Teikyo University School of Medicine, 2-11-1 Kaga, 
      Itabashi-ku, Tokyo 173-8605, Japan. toshwatanabe@yahoo.co.jp
FAU - Kobunai, Takashi
AU  - Kobunai T
FAU - Yamamoto, Yoko
AU  - Yamamoto Y
FAU - Kanazawa, Takamitsu
AU  - Kanazawa T
FAU - Konishi, Tsuyoshi
AU  - Konishi T
FAU - Tanaka, Toshiaki
AU  - Tanaka T
FAU - Matsuda, Keiji
AU  - Matsuda K
FAU - Ishihara, Soichiro
AU  - Ishihara S
FAU - Nozawa, Keijiro
AU  - Nozawa K
FAU - Eshima, Kiyoshi
AU  - Eshima K
FAU - Muto, Tetsuichiro
AU  - Muto T
FAU - Nagawa, Hirokazu
AU  - Nagawa H
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100601
PL  - England
TA  - Eur J Cancer
JT  - European journal of cancer (Oxford, England : 1990)
JID - 9005373
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Colorectal Neoplasms/*genetics
MH  - Female
MH  - Gene Expression
MH  - Genes, Neoplasm/*genetics
MH  - Humans
MH  - Liver Neoplasms/diagnosis/genetics/*secondary
MH  - Male
MH  - Microarray Analysis
MH  - Middle Aged
MH  - Predictive Value of Tests
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Young Adult
EDAT- 2010/06/24 06:00
MHDA- 2010/12/14 06:00
CRDT- 2010/06/24 06:00
PHST- 2010/02/08 00:00 [received]
PHST- 2010/04/19 00:00 [revised]
PHST- 2010/04/20 00:00 [accepted]
PHST- 2010/06/24 06:00 [entrez]
PHST- 2010/06/24 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
AID - S0959-8049(10)00341-2 [pii]
AID - 10.1016/j.ejca.2010.04.019 [doi]
PST - ppublish
SO  - Eur J Cancer. 2010 Jul;46(11):2119-26. doi: 10.1016/j.ejca.2010.04.019. Epub 2010 
      Jun 1.