PMID- 20570346
OWN - NLM
STAT- MEDLINE
DCOM- 20101025
LR  - 20220331
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 31
IP  - 25
DP  - 2010 Sep
TI  - An injectable calcium phosphate-alginate hydrogel-umbilical cord mesenchymal stem 
      cell paste for bone tissue engineering.
PG  - 6502-10
LID - 10.1016/j.biomaterials.2010.05.017 [doi]
AB  - The need for bone repair has increased as the population ages. Stem cell-scaffold 
      approaches hold immense promise for bone tissue engineering. However, currently, 
      preformed scaffolds for cell delivery have drawbacks including the difficulty to 
      seed cells deep into the scaffold, and inability for injection in 
      minimally-invasive surgeries. Current injectable polymeric carriers and hydrogels 
      are too weak for load-bearing orthopedic applications. The objective of this 
      study was to develop an injectable and mechanically-strong stem cell construct 
      for bone tissue engineering. Calcium phosphate cement (CPC) paste was combined 
      with hydrogel microbeads encapsulating human umbilical cord mesenchymal stem 
      cells (hUCMSCs). The hUCMSC-encapsulating composite paste was fully injectable 
      under small injection forces. Cell viability after injection matched that in 
      hydrogel without CPC and without injection. Mechanical properties of the 
      construct matched the reported values of cancellous bone, and were much higher 
      than previous injectable polymeric and hydrogel carriers. hUCMSCs in the 
      injectable constructs osteodifferentiated, yielding high alkaline phosphatase, 
      osteocalcin, collagen type I, and osterix gene expressions at 7 d, which were 
      50-70 fold higher than those at 1 d. Mineralization by the hUCMSCs at 14 d was 
      100-fold that at 1 d. In conclusion, a fully injectable, mechanically-strong, 
      stem cell-CPC scaffold construct was developed. The encapsulated hUCMSCs remained 
      viable, osteodifferentiated, and synthesized bone minerals. The new injectable 
      stem cell construct with load-bearing capability may enhance bone regeneration in 
      minimally-invasive and other orthopedic surgeries.
CI  - Copyright (c) 2010 Elsevier Ltd. All rights reserved.
FAU - Zhao, Liang
AU  - Zhao L
AD  - Department of Endodontics, Prosthodontics and Operative Dentistry, University of 
      Maryland Dental School, Baltimore, MD 21201, USA.
FAU - Weir, Michael D
AU  - Weir MD
FAU - Xu, Hockin H K
AU  - Xu HH
LA  - eng
GR  - R01 DE014190-07A1S1/DE/NIDCR NIH HHS/United States
GR  - R01 DE014190/DE/NIDCR NIH HHS/United States
GR  - R01 DE14190/DE/NIDCR NIH HHS/United States
GR  - R01 DE17974/DE/NIDCR NIH HHS/United States
GR  - R01 DE017974/DE/NIDCR NIH HHS/United States
GR  - R01 DE014190-07A1/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20100608
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Alginates)
RN  - 0 (Calcium Phosphates)
RN  - 0 (Hexuronic Acids)
RN  - 25852-47-5 (Hydrogel, Polyethylene Glycol Dimethacrylate)
RN  - 8A5D83Q4RW (Glucuronic Acid)
RN  - 97Z1WI3NDX (calcium phosphate)
SB  - IM
MH  - Alginates/*administration & dosage/chemistry
MH  - Bone and Bones/cytology
MH  - Calcium Phosphates/*administration & dosage/chemistry
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Glucuronic Acid/administration & dosage/chemistry
MH  - Hexuronic Acids/administration & dosage/chemistry
MH  - Humans
MH  - Hydrogel, Polyethylene Glycol Dimethacrylate/*administration & dosage/chemistry
MH  - Injections
MH  - Materials Testing
MH  - Mesenchymal Stem Cells/*cytology
MH  - *Osteogenesis
MH  - Tissue Engineering/*methods
MH  - Tissue Scaffolds/*chemistry
MH  - Umbilical Cord/cytology
PMC - PMC3001248
MID - NIHMS212202
EDAT- 2010/06/24 06:00
MHDA- 2010/10/26 06:00
PMCR- 2011/09/01
CRDT- 2010/06/24 06:00
PHST- 2010/04/01 00:00 [received]
PHST- 2010/05/13 00:00 [accepted]
PHST- 2010/06/24 06:00 [entrez]
PHST- 2010/06/24 06:00 [pubmed]
PHST- 2010/10/26 06:00 [medline]
PHST- 2011/09/01 00:00 [pmc-release]
AID - S0142-9612(10)00635-6 [pii]
AID - 10.1016/j.biomaterials.2010.05.017 [doi]
PST - ppublish
SO  - Biomaterials. 2010 Sep;31(25):6502-10. doi: 10.1016/j.biomaterials.2010.05.017. 
      Epub 2010 Jun 8.