PMID- 20570883
OWN - NLM
STAT- MEDLINE
DCOM- 20100830
LR  - 20220410
IS  - 1940-6215 (Electronic)
IS  - 1940-6207 (Print)
IS  - 1940-6215 (Linking)
VI  - 3
IP  - 7
DP  - 2010 Jul
TI  - Epidermal growth factor receptor expression and gene copy number in the risk of 
      oral cancer.
PG  - 800-9
LID - 10.1158/1940-6207.CAPR-09-0163 [doi]
AB  - Leukoplakia is the most common premalignant lesion of the oral cavity. Epidermal 
      growth factor receptor (EGFR) abnormalities are associated with oral 
      tumorigenesis and progression. We hypothesized that EGFR expression and gene copy 
      number changes are predictors of the risk of an oral premalignant lesion (OPL) 
      progressing to oral squamous cell carcinoma (OSCC). A formalin-fixed, 
      paraffin-embedded OPL biopsy specimen was collected from each of 162 patients in 
      a randomized controlled clinical trial. We assessed EGFR expression by 
      immunohistochemistry with two METHODS: a semiquantitative analysis (145 evaluable 
      specimens) and an automated quantitative analysis (127 evaluable specimens). EGFR 
      gene copy number was assessed by fluorescence in situ hybridization (FISH) in a 
      subset of 49 OPLs with high EGFR expression defined by the semiquantitative 
      analysis. We analyzed EGFR abnormalities for associations with OSCC development. 
      High EGFR expression occurred in 103 (71%) of the 145 OPLs and was associated 
      with a nonsignificantly higher risk of OSCC (P = 0.10). Twenty (41%) of 49 OPLs 
      assessed by FISH had an increased EGFR gene copy number (FISH-positive). Patients 
      with FISH-positive lesions had a significantly higher incidence of OSCC than did 
      patients with FISH-negative (a normal copy number) lesions (P = 0.0007). Of note, 
      10 of 11 OSCCs that developed at the site of the examined OPL were in the 
      FISH-positive group, leaving only one FISH-negative OPL that did so (P < 0.0001). 
      Our data indicate that an increased EGFR gene copy number is common in and 
      associated with OSCC development in patients with OPLs expressing high EGFR, 
      particularly OSCC developing at the site of a high-expression OPL; they also 
      suggest that EGFR inhibitors may prevent oral cancer in patients with OPLs having 
      an increased EGFR gene copy number.
CI  - 2010 AACR.
FAU - Taoudi Benchekroun, Mohammed
AU  - Taoudi Benchekroun M
AD  - Department of Thoracic/Head and Neck Medical Oncology, The University of Texas M. 
      D. Anderson Cancer Center, Houston, USA.
FAU - Saintigny, Pierre
AU  - Saintigny P
FAU - Thomas, Sufi M
AU  - Thomas SM
FAU - El-Naggar, Adel K
AU  - El-Naggar AK
FAU - Papadimitrakopoulou, Vassiliki
AU  - Papadimitrakopoulou V
FAU - Ren, Hening
AU  - Ren H
FAU - Lang, Wenhua
AU  - Lang W
FAU - Fan, You-Hong
AU  - Fan YH
FAU - Huang, Jianhua
AU  - Huang J
FAU - Feng, Lei
AU  - Feng L
FAU - Lee, J Jack
AU  - Lee JJ
FAU - Kim, Edward S
AU  - Kim ES
FAU - Hong, Waun Ki
AU  - Hong WK
FAU - Johnson, Faye M
AU  - Johnson FM
FAU - Grandis, Jennifer R
AU  - Grandis JR
FAU - Mao, Li
AU  - Mao L
LA  - eng
GR  - P30 CA016672/CA/NCI NIH HHS/United States
GR  - P50 CA097007/CA/NCI NIH HHS/United States
GR  - R01 CA077308/CA/NCI NIH HHS/United States
GR  - P50 CA97007/CA/NCI NIH HHS/United States
GR  - P30 CA16672/CA/NCI NIH HHS/United States
GR  - P01 CA106451/CA/NCI NIH HHS/United States
GR  - P01 CA106451-030003/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20100622
PL  - United States
TA  - Cancer Prev Res (Phila)
JT  - Cancer prevention research (Philadelphia, Pa.)
JID - 101479409
RN  - 0 (Biomarkers, Tumor)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
CIN - Cancer Prev Res (Phila). 2010 Jul;3(7):797-9. PMID: 20570884
MH  - Biomarkers, Tumor/genetics/metabolism
MH  - Carcinoma, Squamous Cell/*epidemiology/genetics/metabolism
MH  - ErbB Receptors/*genetics/*metabolism
MH  - Female
MH  - *Gene Dosage
MH  - Humans
MH  - Leukoplakia, Oral/genetics/metabolism/pathology
MH  - Male
MH  - Middle Aged
MH  - Mouth Neoplasms/*epidemiology/genetics/metabolism
MH  - Precancerous Conditions/genetics/metabolism/*pathology
MH  - Risk Factors
PMC - PMC2900459
MID - NIHMS167990
EDAT- 2010/06/24 06:00
MHDA- 2010/08/31 06:00
PMCR- 2011/07/01
CRDT- 2010/06/24 06:00
PHST- 2010/06/24 06:00 [entrez]
PHST- 2010/06/24 06:00 [pubmed]
PHST- 2010/08/31 06:00 [medline]
PHST- 2011/07/01 00:00 [pmc-release]
AID - 1940-6207.CAPR-09-0163 [pii]
AID - 10.1158/1940-6207.CAPR-09-0163 [doi]
PST - ppublish
SO  - Cancer Prev Res (Phila). 2010 Jul;3(7):800-9. doi: 
      10.1158/1940-6207.CAPR-09-0163. Epub 2010 Jun 22.