PMID- 20571633
OWN - NLM
STAT- MEDLINE
DCOM- 20101203
LR  - 20181201
IS  - 1463-9084 (Electronic)
IS  - 1463-9076 (Linking)
VI  - 12
IP  - 35
DP  - 2010 Sep 21
TI  - Conformational behavior of fibrinogen on topographically modified polymer 
      surfaces.
PG  - 10301-8
LID - 10.1039/c001747g [doi]
AB  - The influence of topographical surface features at the submicron scale on the 
      structural changes in the surface-adsorbed fibrinogen was investigated on 
      poly(lactic-co-glycolic-acid) (PLGA) films. Attenuated total reflection-Fourier 
      transform infrared spectroscopy (ATR-FTIR) was employed in this study for the 
      induced conformational change of fibrinogen over various adsorption times, while 
      the adsorption kinetics of fibrinogen was quantified by the enzyme linked 
      immunosorbent assay (ELISA). When a PLGA surface is modified topographically, the 
      adsorbed fibrinogen undergoes less conformational change when compared to 
      adsorption on the pristine PLGA surface. The extent of conformational change is 
      related to platelet adhesion. Reduced thrombogenicity was demonstrated by the 
      higher ratios of alpha-helix to beta-turn and beta-sheet to beta-turn structures 
      on the topographic PLGA film, which suggests that topographical manipulation of 
      surfaces is a viable approach to influence the thrombogenicity of surfaces.
FAU - Koh, Li Buay
AU  - Koh LB
AD  - School of Materials Science and Engineering, Nanyang Technological University, 
      Singapore, 639798, Singapore.
FAU - Rodriguez, Isabel
AU  - Rodriguez I
FAU - Venkatraman, Subbu S
AU  - Venkatraman SS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100623
PL  - England
TA  - Phys Chem Chem Phys
JT  - Physical chemistry chemical physics : PCCP
JID - 100888160
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 9001-32-5 (Fibrinogen)
SB  - IM
MH  - Adsorption
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Fibrinogen/*chemistry
MH  - Humans
MH  - Kinetics
MH  - Lactic Acid/*chemistry
MH  - Polyglycolic Acid/*chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Protein Conformation
MH  - Spectroscopy, Fourier Transform Infrared
MH  - Surface Properties
EDAT- 2010/06/24 06:00
MHDA- 2010/12/14 06:00
CRDT- 2010/06/24 06:00
PHST- 2010/06/24 06:00 [entrez]
PHST- 2010/06/24 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
AID - 10.1039/c001747g [doi]
PST - ppublish
SO  - Phys Chem Chem Phys. 2010 Sep 21;12(35):10301-8. doi: 10.1039/c001747g. Epub 2010 
      Jun 23.