PMID- 20572074
OWN - NLM
STAT- MEDLINE
DCOM- 20100923
LR  - 20100629
IS  - 1096-9071 (Electronic)
IS  - 0146-6615 (Linking)
VI  - 82
IP  - 8
DP  - 2010 Aug
TI  - Comparison of the levels of human herpesvirus 6 (HHV-6) DNA and cytokines in the 
      cerebrospinal fluid and serum of children with HHV-6 encephalopathy.
PG  - 1410-5
LID - 10.1002/jmv.21808 [doi]
AB  - Primary human herpesvirus-6 (HHV-6) infection is a common cause of acute sporadic 
      encephalopathy in Japanese children. Occasionally, HHV-6 is not detected in the 
      cerebrospinal fluid (CSF) of patients with encephalopathy, for example, in those 
      with focal viral encephalitis, such as herpes simplex viral encephalitis. This 
      indicates that HHV-6 encephalopathy is caused by an indirect mechanism, although 
      this is not fully understood. HHV-6 DNA, cytokines (interleukin (IL)-1beta, IL-6, 
      IL-8, IL-10, IL-12 p70, tumor necrosis factor-alpha, interferon-gamma), and 
      matrix metalloproteinase-9 were quantitated in both the CSF and serum of 13 
      patients with HHV-6 encephalopathy during the acute phase of the disease. HHV-6 
      DNA was detected in the CSF of seven patients with HHV-6 encephalopathy. The 
      viral DNA concentration was significantly higher in serum than in CSF (mean 1.64 
      x 10(4) vs. 5.70 x 10(1) copies/ml; P = 0.003). The lack or low level of viral 
      DNA in the CSF samples suggests that direct invasion of the central nervous 
      system by HHV-6 is not the main cause of encephalopathy. Additionally, the IL-10 
      concentration was significantly higher in serum than in CSF (P < 0.001), whereas 
      there was no significant difference in IL-6 levels between the CSF and serum 
      samples. Interestingly, the IL-8 concentration was significantly higher in CSF 
      than in serum (P = 0.038). The distribution of these cytokines differed between 
      CSF and serum. The high CSF concentration of IL-8 could play an important role in 
      the pathogenesis of encephalopathy.
CI  - (c) 2010 Wiley-Liss, Inc.
FAU - Kawabe, Shinji
AU  - Kawabe S
AD  - Department of Pediatrics, Nagoya University Graduate School of Medicine, Nagoya, 
      Japan.
FAU - Ito, Yoshinori
AU  - Ito Y
FAU - Ohta, Rieko
AU  - Ohta R
FAU - Sofue, Ayako
AU  - Sofue A
FAU - Gotoh, Kensei
AU  - Gotoh K
FAU - Morishima, Tsuneo
AU  - Morishima T
FAU - Kimura, Hiroshi
AU  - Kimura H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Med Virol
JT  - Journal of medical virology
JID - 7705876
RN  - 0 (Cytokines)
RN  - 0 (DNA, Viral)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Cerebrospinal Fluid/*immunology/*virology
MH  - Child, Preschool
MH  - Cytokines/*cerebrospinal fluid
MH  - DNA, Viral/blood/cerebrospinal fluid
MH  - Encephalitis, Viral/*immunology/*virology
MH  - Female
MH  - Herpesvirus 6, Human/*isolation & purification
MH  - Humans
MH  - Infant
MH  - Japan
MH  - Male
MH  - Matrix Metalloproteinase 9/cerebrospinal fluid
MH  - Roseolovirus Infections/*immunology/virology
MH  - Viral Load
EDAT- 2010/06/24 06:00
MHDA- 2010/09/24 06:00
CRDT- 2010/06/24 06:00
PHST- 2010/06/24 06:00 [entrez]
PHST- 2010/06/24 06:00 [pubmed]
PHST- 2010/09/24 06:00 [medline]
AID - 10.1002/jmv.21808 [doi]
PST - ppublish
SO  - J Med Virol. 2010 Aug;82(8):1410-5. doi: 10.1002/jmv.21808.