PMID- 20572766
OWN - NLM
STAT- MEDLINE
DCOM- 20110214
LR  - 20171116
IS  - 1943-3670 (Electronic)
IS  - 0022-3492 (Linking)
VI  - 81
IP  - 10
DP  - 2010 Oct
TI  - Gingival RAGE expression in calorie-restricted versus ad libitum-fed rats.
PG  - 1481-7
LID - 10.1902/jop.2010.100019 [doi]
AB  - BACKGROUND: The receptor for advanced glycation end products (RAGE) is a 
      multiligand receptor expressed in a number of cell types, including gingival 
      epithelia. RAGE mediates inflammation and induces cellular oxidative stress. 
      Upregulation of RAGE is associated with various diseases, such as periodontal and 
      cardiovascular diseases. This study examines the hypothesis that the gingiva of 
      rats fed a calorie-restriction (CR) diet expresses lower levels of RAGE than the 
      gingiva of rats fed an ad libitum (AL) diet. METHODS: Male F344BN rats (n = 16) 
      from the National Institute on Aging (NIA) were fed a CR (n = 8) or AL (n = 8) 
      diet according to NIA recommendations. Rats were sacrificed by guillotine at 8 (n 
      = 5), 18 (n = 3), 29 (n = 4), and 38 (n = 4) months of age. The gingiva from 
      around the molars was dissected and submitted for histologic and molecular 
      analyses. RESULTS: Immunohistochemistry revealed that RAGE was expressed in the 
      plasma membrane and cytoplasm of gingival epithelial cells and endothelial cells 
      from both groups. RAGE mRNA levels were quantified relative to levels of GAPDH 
      mRNA by real-time reverse-transcriptase polymerase chain reaction. The mean 
      relative RAGE mRNA level in the CR group (7.26 +/- 0.54) was lower than in the AL 
      group (10 +/- 1.4) (P <0.05). There were no detectable differences in RAGE 
      expression according to animal age. CONCLUSION: Gingival RAGE expression in rats 
      is reduced by calorie restriction.
FAU - Aris, John P
AU  - Aris JP
AD  - Department of Anatomy and Cell Biology, College of Medicine, University of 
      Florida, Gainesville, FL 32610-0414, USA.
FAU - Elios, Megan C
AU  - Elios MC
FAU - Bimstein, Enrique
AU  - Bimstein E
FAU - Wallet, Shanon M
AU  - Wallet SM
FAU - Cha, Seunghee
AU  - Cha S
FAU - Lakshmyya, Kesavalu N
AU  - Lakshmyya KN
FAU - Katz, Joseph
AU  - Katz J
LA  - eng
GR  - AG023719/AG/NIA NIH HHS/United States
GR  - U24 DE 016509/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - J Periodontol
JT  - Journal of periodontology
JID - 8000345
RN  - 0 (Glycation End Products, Advanced)
RN  - 0 (Receptor for Advanced Glycation End Products)
RN  - 0 (Receptors, Immunologic)
SB  - IM
MH  - Animals
MH  - *Caloric Restriction
MH  - Epithelial Cells/metabolism
MH  - Gene Expression
MH  - Gingiva/cytology/*metabolism
MH  - Glycation End Products, Advanced/*metabolism
MH  - Male
MH  - Rats
MH  - Rats, Inbred F344
MH  - Receptor for Advanced Glycation End Products
MH  - Receptors, Immunologic/*biosynthesis/genetics
EDAT- 2010/06/25 06:00
MHDA- 2011/02/15 06:00
CRDT- 2010/06/25 06:00
PHST- 2010/06/25 06:00 [entrez]
PHST- 2010/06/25 06:00 [pubmed]
PHST- 2011/02/15 06:00 [medline]
AID - 10.1902/jop.2010.100019 [doi]
PST - ppublish
SO  - J Periodontol. 2010 Oct;81(10):1481-7. doi: 10.1902/jop.2010.100019.