PMID- 20573302
OWN - NLM
STAT- MEDLINE
DCOM- 20110131
LR  - 20101019
IS  - 1555-3892 (Electronic)
IS  - 0963-6897 (Linking)
VI  - 19
IP  - 6
DP  - 2010
TI  - Brain death in combination with warm ischemic stress during isolation procedures 
      induces the expression of crucial inflammatory mediators in the isolated islets.
PG  - 775-82
LID - 10.3727/096368910X508889 [doi]
AB  - Tissue factor (TF) and monocyte chemoattractant protein-1 (MCP-1) expressed on 
      the islets have been identified as the main trigger of the instant blood-mediated 
      inflammatory reaction (IBMIR) in islet transplantation. Because the key steps 
      that directly induce TF and MCP-1 remain to be determined, we focused on the 
      influence of brain death (BD) on TF and MCP-1 expression in the pancreatic 
      tissues and isolated islets using a rodent model. TF and MCP-1 mRNA levels in the 
      pancreatic tissues were similar between the BD and the control group. However, TF 
      and MCP-1 mRNA in the fresh islets of the BD group were significantly higher than 
      that of the control group (p < 0.01). BD may thus be suggested to be of great 
      importance as an initiator of TF and MCP-1 induction in the isolated islets. 
      Furthermore, the upregulation of crucial inflammatory mediators induced by BD 
      could be exacerbated by warm ischemic damage during digestion procedures. In the 
      present study, the islet yield and purity were affected by BD. However, almost no 
      influences were observed with respect to islet viability, indicating that the 
      expression of inflammatory mediators rather than islet viability is more 
      susceptible to BD. According to the change in time course of TF and MCP-1 
      expression in the isolated islets, the selected time point for islet infusion in 
      current clinical islet transplantation was thus shown to be at its worst level, 
      at least with respect to the damage caused by BD and ischemic stress. In 
      conclusion, BD in combination with warm ischemic stress during isolation 
      procedures induces a high expression of TF and MCP-1 in the isolated islets. In 
      order to reduce the expression of crucial inflammatory mediators in the islet 
      grafts, the management of the pancreas from brain-dead donors with early 
      anti-inflammatory treatments is thus warranted.
FAU - Saito, Yukihiko
AU  - Saito Y
AD  - Division of Advanced Surgical Science and Technology, Tohoku University, Sendai, 
      Japan.
FAU - Goto, Masafumi
AU  - Goto M
FAU - Maya, Kozue
AU  - Maya K
FAU - Ogawa, Norihiko
AU  - Ogawa N
FAU - Fujimori, Keisei
AU  - Fujimori K
FAU - Kurokawa, Yoshimochi
AU  - Kurokawa Y
FAU - Satomi, Susumu
AU  - Satomi S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100623
PL  - United States
TA  - Cell Transplant
JT  - Cell transplantation
JID - 9208854
RN  - 0 (Chemokine CCL2)
RN  - 0 (Inflammation Mediators)
RN  - 0 (RNA, Messenger)
RN  - 9035-58-9 (Thromboplastin)
SB  - IM
MH  - Animals
MH  - Brain Death/*pathology
MH  - Chemokine CCL2/genetics/metabolism
MH  - Gene Expression Regulation
MH  - Inflammation Mediators/*metabolism
MH  - Islets of Langerhans/*metabolism
MH  - Male
MH  - Organ Preservation/*methods
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Thromboplastin/genetics/metabolism
MH  - Time Factors
MH  - Tissue Survival
MH  - *Warm Ischemia
EDAT- 2010/06/25 06:00
MHDA- 2011/02/01 06:00
CRDT- 2010/06/25 06:00
PHST- 2010/06/25 06:00 [entrez]
PHST- 2010/06/25 06:00 [pubmed]
PHST- 2011/02/01 06:00 [medline]
AID - ct2266saito [pii]
AID - 10.3727/096368910X508889 [doi]
PST - ppublish
SO  - Cell Transplant. 2010;19(6):775-82. doi: 10.3727/096368910X508889. Epub 2010 Jun 
      23.