PMID- 20573395
OWN - NLM
STAT- MEDLINE
DCOM- 20101007
LR  - 20100712
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Linking)
VI  - 31
IP  - 27
DP  - 2010 Sep
TI  - Preservation of dendritic cell function upon labeling with amino functionalized 
      polymeric nanoparticles.
PG  - 7086-95
LID - 10.1016/j.biomaterials.2010.05.073 [doi]
AB  - Dendritic cells (DCs) are key players in eliciting immunity against antigens, 
      therefore making them the focus of many investigations on immune responses in 
      infections, cancer and autoimmune diseases. Nanosized materials have just 
      recently been investigated for their use as carriers of antigens and as labeling 
      agents for DCs. For this later use nanoparticles should be non-toxic and should 
      most importantly not alter the physiological functions of DCs. Here we 
      demonstrate that by the use of polymeric fluorescent nanoparticles as synthesized 
      by the miniemulsion process immature DCs (iDCs) can be efficiently labeled 
      intracellularly. Amino functionalized nanoparticles are more effective than 
      carboxy functionalized ones. Even after 8 days 95% of DCs have retained 
      nanoparticles with a fluorescence intensity of 67% compared to day 1. 
      Nanoparticle labeling does not influence expression of cell surface molecules on 
      mature DCs (mDCs) like HLA-DR, CD80/83/86, CCR7, CD11c nor does it influence the 
      immunostimulatory capacity of mDCs. This procedure does also not impair the 
      capability of DCs for uptake, processing and presentation of viral antigens as 
      demonstrated by interferon-gamma ELISPOT on T cells stimulated with viral 
      antigens presented by DCs. Therefore polymeric nanoparticles are a promising tool 
      to study migration and homing of DCs in animal studies.
CI  - Copyright (c) 2010 Elsevier Ltd. All rights reserved.
FAU - Zupke, O
AU  - Zupke O
AD  - Department of Medicine III, Hematology and Oncology, University Medical Center of 
      the Johannes Gutenberg-University Mainz, Langenbeckstrasse 1, Mainz, Germany.
FAU - Distler, E
AU  - Distler E
FAU - Baumann, D
AU  - Baumann D
FAU - Strand, D
AU  - Strand D
FAU - Meyer, R G
AU  - Meyer RG
FAU - Landfester, K
AU  - Landfester K
FAU - Herr, W
AU  - Herr W
FAU - Mailander, V
AU  - Mailander V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100622
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Polystyrenes)
SB  - IM
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - CD8-Positive T-Lymphocytes/microbiology
MH  - Cells, Cultured
MH  - Dendritic Cells/drug effects/*immunology/*metabolism
MH  - Flow Cytometry
MH  - Humans
MH  - Microscopy, Confocal
MH  - Nanoparticles/adverse effects/*chemistry
MH  - Polystyrenes/*chemistry
EDAT- 2010/06/25 06:00
MHDA- 2010/10/12 06:00
CRDT- 2010/06/25 06:00
PHST- 2010/05/04 00:00 [received]
PHST- 2010/05/27 00:00 [accepted]
PHST- 2010/06/25 06:00 [entrez]
PHST- 2010/06/25 06:00 [pubmed]
PHST- 2010/10/12 06:00 [medline]
AID - S0142-9612(10)00726-X [pii]
AID - 10.1016/j.biomaterials.2010.05.073 [doi]
PST - ppublish
SO  - Biomaterials. 2010 Sep;31(27):7086-95. doi: 10.1016/j.biomaterials.2010.05.073. 
      Epub 2010 Jun 22.