PMID- 20573882
OWN - NLM
STAT- MEDLINE
DCOM- 20100715
LR  - 20220309
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 30
IP  - 25
DP  - 2010 Jun 23
TI  - Aplysia cell adhesion molecule and a novel protein kinase C activity in the 
      postsynaptic neuron are required for presynaptic growth and initial formation of 
      specific synapses.
PG  - 8353-66
LID - 10.1523/JNEUROSCI.0546-10.2010 [doi]
AB  - To explore the role of both Aplysia cell adhesion molecule (ApCAM) and activity 
      of specific protein kinase C (PKC) isoforms in the initial formation of sensory 
      neuron synapses with specific postsynaptic targets (L7 but not L11), we examined 
      presynaptic growth, initial synapse formation, and the expression of the 
      presynaptic neuropeptide sensorin following cell-specific reduction of ApCAM or 
      of a novel PKC activity. Synapse formation between sensory neurons and L7 begins 
      by 3 h after plating and is accompanied by a rapid accumulation of a novel PKC to 
      sites of synaptic interaction. Reducing ApCAM expression specifically from the 
      surface of L7 blocks presynaptic growth and initial synapse formation, 
      target-induced increase of sensorin in sensory neuron cell bodies and the rapid 
      accumulation of the novel PKC to sites of interaction. Selective blockade of the 
      novel PKC activity in L7, but not in sensory neurons, with injection of a 
      dominant negative construct that interferes with the novel PKC activity, produces 
      the same actions as downregulating ApCAM; blockade of presynaptic growth and 
      initial synapse formation, and the target-induced increase of sensorin in sensory 
      neuron cell bodies. The results indicate that signals initiated by postsynaptic 
      cell adhesion molecule ApCAM coupled with the activation of a novel PKC in the 
      appropriate postsynaptic neuron produce the retrograde signals required for 
      presynaptic growth associated with initial synapse formation, and the 
      target-induced expression of a presynaptic neuropeptide critical for synapse 
      maturation.
FAU - Hu, Jiang-Yuan
AU  - Hu JY
AD  - Department of Neuroscience, Columbia University College of Physicians and 
      Surgeons, New York State Psychiatric Institute, New York, New York 10032, USA.
FAU - Chen, Yang
AU  - Chen Y
FAU - Bougie, Joanna K
AU  - Bougie JK
FAU - Sossin, Wayne S
AU  - Sossin WS
FAU - Schacher, Samuel
AU  - Schacher S
LA  - eng
GR  - R01 NS042159-08/NS/NINDS NIH HHS/United States
GR  - R01 MH060387/MH/NIMH NIH HHS/United States
GR  - R01 NS042159/NS/NINDS NIH HHS/United States
GR  - R01 MH060387-11/MH/NIMH NIH HHS/United States
GR  - MH 60387/MH/NIMH NIH HHS/United States
GR  - P40 RR010294/RR/NCRR NIH HHS/United States
GR  - NS 42159/NS/NINDS NIH HHS/United States
GR  - MOP12046/CAPMC/CIHR/Canada
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Neuropeptides)
RN  - 0 (sensorin A protein, Aplysia)
RN  - EC 2.7.11.13 (Protein Kinase C)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Aplysia/growth & development/metabolism
MH  - Cell Adhesion Molecules/*metabolism
MH  - Cells, Cultured
MH  - Electrophysiology
MH  - Fluorescent Dyes
MH  - Ganglia, Invertebrate/metabolism
MH  - Immunohistochemistry
MH  - Microscopy, Fluorescence
MH  - Neurons/cytology/*metabolism
MH  - Neuropeptides/metabolism
MH  - Presynaptic Terminals/*metabolism
MH  - Protein Kinase C/*metabolism
MH  - Synapses/*metabolism
MH  - Synaptic Transmission/physiology
PMC - PMC2908027
MID - NIHMS216044
EDAT- 2010/06/25 06:00
MHDA- 2010/07/16 06:00
PMCR- 2010/12/23
CRDT- 2010/06/25 06:00
PHST- 2010/06/25 06:00 [entrez]
PHST- 2010/06/25 06:00 [pubmed]
PHST- 2010/07/16 06:00 [medline]
PHST- 2010/12/23 00:00 [pmc-release]
AID - 30/25/8353 [pii]
AID - 3607154 [pii]
AID - 10.1523/JNEUROSCI.0546-10.2010 [doi]
PST - ppublish
SO  - J Neurosci. 2010 Jun 23;30(25):8353-66. doi: 10.1523/JNEUROSCI.0546-10.2010.