PMID- 20574022
OWN - NLM
STAT- MEDLINE
DCOM- 20110113
LR  - 20220311
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 51
IP  - 12
DP  - 2010 Dec
TI  - Reproducibility of spectral-domain optical coherence tomography total retinal 
      thickness measurements in mice.
PG  - 6519-23
LID - 10.1167/iovs.10-5662 [doi]
AB  - PURPOSE: To test the reproducibility of spectral-domain optical coherence 
      tomography (SD-OCT) total retinal thickness (TRT) measurements in mice. METHODS: 
      C57Bl/6 mice were anesthetized, and three repeated volumetric images were 
      acquired in both eyes with SD-OCT (250 A-scans x 250 frames x 1024 samplings), 
      centered on the optic nerve head (ONH). The mice were repositioned between scans. 
      TRT was automatically measured within a sampling band of retinal thickness with 
      radii of 55 to 70 pixels, centered on the ONH by using custom segmentation 
      software. The first volumetric image acquired in a given eye was used to register 
      the remaining two SD-OCT images by manually aligning the en face images with 
      respect to rotation and linear translation. Linear mixed-effects models were 
      fitted to global and quadrant thicknesses, taking into account the clustering 
      between eyes, to assess imprecision (measurement reproducibility). RESULTS: 
      Twenty-six eyes of 13 adult mice (age 13 weeks) were imaged. The mean global TRT 
      across all eyes was 298.21 mum, with a mouse heterogeneity standard deviation (SD) 
      of 4.88 mum (coefficient of variation [CV] = 0.016), an eye SD of 3.32 mum (CV = 
      0.011), and a device-related imprecision SD of 2.33 mum (CV = 0.008). The superior 
      quadrant had the thickest mean TRT measurement (310.38 mum) and the highest 
      (worst) imprecision SD (3.13 mum; CV = 0.010), and the inferior quadrant had the 
      thinnest mean TRT (291.55 mum). The quadrant with the lowest (best) imprecision SD 
      was in the nasal one (2.06 mum; CV = 0.007). CONCLUSIONS: Good reproducibility was 
      observed for SD-OCT retinal thickness measurements in mice. SD-OCT may be useful 
      for in vivo longitudinal studies in mice.
FAU - Gabriele, Michelle L
AU  - Gabriele ML
AD  - UPMC Eye Center, Eye and Ear Institute, Department of Ophthalmology, University 
      of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.
FAU - Ishikawa, Hiroshi
AU  - Ishikawa H
FAU - Schuman, Joel S
AU  - Schuman JS
FAU - Bilonick, Richard A
AU  - Bilonick RA
FAU - Kim, Jongsick
AU  - Kim J
FAU - Kagemann, Larry
AU  - Kagemann L
FAU - Wollstein, Gadi
AU  - Wollstein G
LA  - eng
GR  - R21 EY019092/EY/NEI NIH HHS/United States
GR  - R21 EY019092-01A2/EY/NEI NIH HHS/United States
GR  - R01-EY013178/EY/NEI NIH HHS/United States
GR  - T32 EY017271-02/EY/NEI NIH HHS/United States
GR  - P30-EY008098/EY/NEI NIH HHS/United States
GR  - T32-EY017271/EY/NEI NIH HHS/United States
GR  - R21-EY019092/EY/NEI NIH HHS/United States
GR  - T32 EY017271/EY/NEI NIH HHS/United States
GR  - P30 EY008098/EY/NEI NIH HHS/United States
GR  - P30 EY008098-20/EY/NEI NIH HHS/United States
GR  - R01 EY013178/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20100623
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
SB  - IM
MH  - Animals
MH  - Anthropometry
MH  - Biometry
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Reproducibility of Results
MH  - Retina/*anatomy & histology
MH  - Tomography, Optical Coherence/*instrumentation
MH  - Tumor Protein, Translationally-Controlled 1
PMC - PMC3055768
EDAT- 2010/06/25 06:00
MHDA- 2011/01/14 06:00
PMCR- 2011/06/01
CRDT- 2010/06/25 06:00
PHST- 2010/06/25 06:00 [entrez]
PHST- 2010/06/25 06:00 [pubmed]
PHST- 2011/01/14 06:00 [medline]
PHST- 2011/06/01 00:00 [pmc-release]
AID - iovs.10-5662 [pii]
AID - 10-5662 [pii]
AID - 10.1167/iovs.10-5662 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2010 Dec;51(12):6519-23. doi: 10.1167/iovs.10-5662. 
      Epub 2010 Jun 23.