PMID- 20574121
OWN - NLM
STAT- MEDLINE
DCOM- 20110216
LR  - 20231213
IS  - 1735-1383 (Print)
IS  - 1735-1383 (Linking)
VI  - 7
IP  - 2
DP  - 2010 Jun
TI  - Comparison of several maturation inducing factors in dendritic cell 
      differentiation.
PG  - 83-7
AB  - BACKGROUND: Dendritic cells (DCs) are professional antigen presenting cells that 
      have an important role in the initiation of immune response. The use of 
      maturation factors in dendritic cell differentiation provides a promising 
      approach in immunotherapy. OBJECTIVE: In this study, we compared tumor necrosis 
      factor-alpha, polyribocytidylic acid, lipopolysacharide and CpG oligonucleotides in 
      inducing dendritic cell maturation. METHODS: We generated immature dendritic 
      cells with GM-CSF in combination with IL-4 from peripheral blood mononuclear 
      adherent cells and used tumor necrosis factor-alpha, polyribocytidylic acid, 
      lipopolysacharide and CpG for the induction of dendritic cell maturation. CD83 
      maturation marker on the dendritic cells was analyzed by flowcytometry after 7 
      days. In addition, mixed leukocyte reaction between dendritic cells and T cells 
      was performed by MTT proliferation assay. RESULTS: Flow cytometry results 
      demonstrated a comparable high level of CD83 expression on the mature dendritic 
      cells generated by TNF-alpha, CpG, Poly I:C, and LPS treatment of the immature 
      dendritic cells. However, a significantly poorer proliferation of lymphocytes 
      cocultured with the Poly I:C-treated DCs was observed compared to the CpG-treated 
      DCs in mixed leukocyte reaction (p=0.026). Conversely, a significantly stronger 
      proliferation of lymphocytes was observed when cocultured with TNF-alpha-treated DCs 
      compared to the LPS-treated DCs (p=0.025). CONCLUSION: Our results indicated that 
      all of studied maturation inducing factors can be used in DC maturation but TNF-alpha 
      and CpG were the preferred in vitro maturation factors. It is concluded that 
      maturation of dendritic cells by CpG motif and TNF-alpha can be used to regulate 
      immune responses.
FAU - Abediankenari, Saeid
AU  - Abediankenari S
AD  - Department of Microbiology and Immunology, Mazandaran University of Medical 
      Sciences, Sari, Iran. abedianks@razi.tums.ac.ir
FAU - Yousefzadeh, Yousef
AU  - Yousefzadeh Y
FAU - Azadeh, Hossein
AU  - Azadeh H
FAU - Vahedi, Mohammad
AU  - Vahedi M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Iran
TA  - Iran J Immunol
JT  - Iranian journal of immunology : IJI
JID - 101282932
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation)
RN  - 0 (CPG-oligonucleotide)
RN  - 0 (Immunoglobulins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - O84C90HH2L (Poly I-C)
SB  - IM
MH  - Antigen Presentation/drug effects
MH  - Antigens, CD/genetics/metabolism
MH  - Antigens, Differentiation/genetics/metabolism
MH  - CD4-Positive T-Lymphocytes/cytology/immunology/*metabolism
MH  - Cell Differentiation/drug effects
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Dendritic Cells/cytology/*drug effects/immunology/metabolism
MH  - Humans
MH  - Immunoglobulins/genetics/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Lymphocyte Activation/drug effects
MH  - Lymphocyte Culture Test, Mixed
MH  - Membrane Glycoproteins/genetics/metabolism
MH  - Oligodeoxyribonucleotides/*pharmacology
MH  - Poly I-C/pharmacology
MH  - Tumor Necrosis Factor-alpha/*pharmacology
MH  - CD83 Antigen
EDAT- 2010/06/25 06:00
MHDA- 2011/02/17 06:00
CRDT- 2010/06/25 06:00
PHST- 2010/06/25 06:00 [entrez]
PHST- 2010/06/25 06:00 [pubmed]
PHST- 2011/02/17 06:00 [medline]
AID - 03 [pii]
PST - ppublish
SO  - Iran J Immunol. 2010 Jun;7(2):83-7.