PMID- 20575089
OWN - NLM
STAT- MEDLINE
DCOM- 20101123
LR  - 20211020
IS  - 1099-0496 (Electronic)
IS  - 8755-6863 (Print)
IS  - 1099-0496 (Linking)
VI  - 45
IP  - 7
DP  - 2010 Jul
TI  - Pseudomonas aeruginosa in children with cystic fibrosis diagnosed through newborn 
      screening: assessment of clinic exposures and microbial genotypes.
PG  - 708-16
LID - 10.1002/ppul.21263 [doi]
AB  - BACKGROUND: Chronic pulmonary infection with Pseudomonas aeruginosa (PA) is 
      responsible for significant morbidity and mortality in cystic fibrosis (CF). 
      Because of the limited studies evaluating early exposure and the progression of 
      genetic variability of PA, our goal was to assess PA in young children with CF 
      followed in two clinic types. METHODS: A total of 39 infants with CF diagnosed 
      through newborn screening were randomly assigned to either a segregated (PA-free) 
      or mixed (PA-positive) clinic at two different CF centers, one of which replaced 
      an older, mixed clinic where nosocomial acquisition was suspected. Oropharyngeal 
      (OP) swab cultures were examined with subsequent genotyping to characterize the 
      strains of PA isolated. RESULTS: We found that 13/21 segregated clinic patients 
      and 14/18 mixed clinic patients showed positive PA, with median acquisition ages 
      of 3.3 and 2.2 years, respectively (P = 0.57). The median time to PA acquisition, 
      however, was significantly longer in the new clinic with proper hygiene 
      precautions compared to an old site (5.0 years vs. 1.7 years, P < 0.001). The 
      majority of subjects isolated a single genotype of PA or AP-PCR types during the 
      study period with eight subjects clearing the isolate after only one positive 
      culture. The development of chronic colonization yielded the predominance of a 
      single major genotype or AP-PCR type. CONCLUSIONS: Segregation of infants and 
      young children with CF in PA-negative or PA-positive clinics did not alter the 
      time to first PA isolation in this randomized assessment of facilities with 
      hygienic precautions. During the early infection period where PA is first 
      isolated in young children with CF, patients cleared different PA strains until a 
      predominant strain established permanent colonization.
FAU - Hayes, Don Jr
AU  - Hayes D Jr
AD  - Department of Pediatrics and Internal Medicine, University of Kentucky College of 
      Medicine, Lexington, KY, USA.
FAU - West, Susan E
AU  - West SE
FAU - Rock, Michael J
AU  - Rock MJ
FAU - Li, Zhanhai
AU  - Li Z
FAU - Splaingard, Mark L
AU  - Splaingard ML
FAU - Farrell, Philip M
AU  - Farrell PM
LA  - eng
GR  - R01 DK034108/DK/NIDDK NIH HHS/United States
GR  - R01 DK034108-17/DK/NIDDK NIH HHS/United States
GR  - 5 R01DK34108-17/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Pediatr Pulmonol
JT  - Pediatric pulmonology
JID - 8510590
SB  - IM
MH  - Ambulatory Care Facilities
MH  - Child
MH  - Child, Preschool
MH  - Chronic Disease
MH  - Cross Infection/microbiology
MH  - Cystic Fibrosis/*microbiology
MH  - Female
MH  - Humans
MH  - Infant
MH  - Infant, Newborn
MH  - Longitudinal Studies
MH  - Male
MH  - *Neonatal Screening
MH  - Oropharynx/microbiology
MH  - Pseudomonas Infections/*diagnosis/*transmission
MH  - Pseudomonas aeruginosa/*genetics/isolation & purification
PMC - PMC2921980
MID - NIHMS225153
EDAT- 2010/06/25 06:00
MHDA- 2010/12/14 06:00
PMCR- 2011/07/01
CRDT- 2010/06/25 06:00
PHST- 2010/06/25 06:00 [entrez]
PHST- 2010/06/25 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
PHST- 2011/07/01 00:00 [pmc-release]
AID - 10.1002/ppul.21263 [doi]
PST - ppublish
SO  - Pediatr Pulmonol. 2010 Jul;45(7):708-16. doi: 10.1002/ppul.21263.