PMID- 20576928
OWN - NLM
STAT- MEDLINE
DCOM- 20110427
LR  - 20220224
IS  - 1460-2199 (Electronic)
IS  - 1047-3211 (Print)
IS  - 1047-3211 (Linking)
VI  - 21
IP  - 2
DP  - 2011 Feb
TI  - L1 and CHL1 Cooperate in Thalamocortical Axon Targeting.
PG  - 401-12
LID - 10.1093/cercor/bhq115 [doi]
AB  - Neural cell adhesion molecule close homolog of L1 (CHL1) is a regulator of 
      topographic targeting of thalamic axons to the somatosensory cortex (S1) but 
      little is known about its cooperation with other L1 class molecules. To 
      investigate this, CHL1(-/-)/L1(-/y) double mutant mice were generated and 
      analyzed for thalamocortical axon topography. Double mutants exhibited a striking 
      posterior shift of axons from motor thalamic nuclei to the visual cortex (V1), 
      which was not observed in single mutants. In wild-type (WT) embryos, L1 and CHL1 
      were coexpressed in the dorsal thalamus (DT) and on fibers along the 
      thalamocortical projection in the ventral telencephalon and cortex. L1 and CHL1 
      colocalized on growth cones and neurites of cortical and thalamic neurons in 
      culture. Growth cone collapse assays with WT and mutant neurons demonstrated a 
      requirement for L1 and CHL1 in repellent responses to EphrinA5, a guidance factor 
      for thalamic axons. L1 coimmunoprecipitated with the principal EphrinA5 receptors 
      expressed in the DT (EphA3, EphA4, and EphA7), whereas CHL1 associated 
      selectively with EphA7. These results implicate a novel mechanism in which L1 and 
      CHL1 interact with individual EphA receptors and cooperate to guide 
      subpopulations of thalamic axons to distinct neocortical areas essential for 
      thalamocortical connectivity.
FAU - Demyanenko, Galina P
AU  - Demyanenko GP
AD  - Department of Biochemistry and Biophysics, University of North Carolina School of 
      Medicine, Chapel Hill, NC 27599, USA.
FAU - Siesser, Priscila F
AU  - Siesser PF
FAU - Wright, Amanda G
AU  - Wright AG
FAU - Brennaman, Leann H
AU  - Brennaman LH
FAU - Bartsch, Udo
AU  - Bartsch U
FAU - Schachner, Melitta
AU  - Schachner M
FAU - Maness, Patricia F
AU  - Maness PF
LA  - eng
GR  - MH064056/MH/NIMH NIH HHS/United States
GR  - NS049109/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20100624
PL  - United States
TA  - Cereb Cortex
JT  - Cerebral cortex (New York, N.Y. : 1991)
JID - 9110718
RN  - 0 (Amino Acids)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Chl1 protein, mouse)
RN  - 0 (Ephrin-A5)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Neural Cell Adhesion Molecule L1)
RN  - 0 (dolaisoleucine)
RN  - EC 2.7.10.1 (Receptors, Eph Family)
SB  - IM
MH  - Amino Acids/metabolism
MH  - Animals
MH  - Axons/drug effects/*physiology/ultrastructure
MH  - Cell Adhesion Molecules/deficiency/*metabolism
MH  - Cells, Cultured
MH  - Cerebral Cortex/cytology/*metabolism
MH  - Embryo, Mammalian
MH  - Ephrin-A5/pharmacology
MH  - Gene Expression Regulation/drug effects/genetics
MH  - Growth Cones/physiology
MH  - Humans
MH  - Immunoprecipitation/methods
MH  - Luminescent Proteins/genetics
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Neural Cell Adhesion Molecule L1/deficiency/*metabolism
MH  - Neural Pathways/*physiology
MH  - Neurons/cytology/drug effects/physiology
MH  - Receptors, Eph Family/genetics/metabolism
MH  - Thalamus/cytology/*metabolism
MH  - Transfection/methods
PMC - PMC3020587
EDAT- 2010/06/26 06:00
MHDA- 2011/04/28 06:00
PMCR- 2012/02/01
CRDT- 2010/06/26 06:00
PHST- 2010/06/26 06:00 [entrez]
PHST- 2010/06/26 06:00 [pubmed]
PHST- 2011/04/28 06:00 [medline]
PHST- 2012/02/01 00:00 [pmc-release]
AID - bhq115 [pii]
AID - 10.1093/cercor/bhq115 [doi]
PST - ppublish
SO  - Cereb Cortex. 2011 Feb;21(2):401-12. doi: 10.1093/cercor/bhq115. Epub 2010 Jun 
      24.