PMID- 20579220
OWN - NLM
STAT- MEDLINE
DCOM- 20100929
LR  - 20100628
IS  - 1751-2980 (Electronic)
IS  - 1751-2972 (Linking)
VI  - 11
IP  - 3
DP  - 2010 Jun
TI  - Differential gene and protein expression in primary gastric carcinomas and their 
      lymph node metastases as revealed by combined cDNA microarray and tissue 
      microarray analysis.
PG  - 167-75
LID - 10.1111/j.1751-2980.2010.00432.x [doi]
AB  - OBJECTIVE: To gain insight into the molecular events of lymph node metastasis of 
      human gastric carcinoma. METHODS: The gene expression profile of five matched 
      primary gastric carcinomas and their lymph node metastases was analyzed by 
      complementary DNA (cDNA) microarray. Differential genes were identified in the 
      metastatic and corresponding primary tumor pairs. Among the differentially 
      expressed genes, carbonic anhydrase II (CAII) and insulin-like growth factor 
      binding protein 4 (IGFBP 4) genes were detected by RT-PCR. CTTN protein 
      expression was examined by tissue microarray. RESULTS: There was a high 
      expression (over twofold) of 44 genes and a low expression (under twofold) of 32 
      genes in lymph node metastasis compared with primary gastric carcinoma, 
      respectively. CAII mRNA was downregulated and IGFBP 4 mRNA was upregulated in 
      paired lymph node metastases of gastric carcinomas. The overexpression of CTTN 
      protein was related to the lymph node metastasis and the clinical stage of 
      gastric carcinomas. CONCLUSION: This study showed that there is a low expression 
      of genes relative to growth signal and immune response in lymph node metastases, 
      and a high expression of genes relative to growth factor, cell cycle, cell 
      motility and adhesion in lymph node metastases compared with primary gastric 
      carcinomas. The expression of CTTN was related to the invasion and metastasis of 
      gastric cancer.
FAU - Xie, Hai Long
AU  - Xie HL
AD  - Cancer Research Institute, Medical College of University of South China, 
      Hengyang, Hunan Province, China.
FAU - Li, Zhi Yu
AU  - Li ZY
FAU - Gan, Run Liang
AU  - Gan RL
FAU - Li, Xiao Jie
AU  - Li XJ
FAU - Zhang, Qing Li
AU  - Zhang QL
FAU - Hui, Mei
AU  - Hui M
FAU - Zhou, Xiu Tian
AU  - Zhou XT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - J Dig Dis
JT  - Journal of digestive diseases
JID - 101302699
RN  - 0 (CTTN protein, human)
RN  - 0 (Cortactin)
RN  - 0 (Insulin-Like Growth Factor Binding Protein 4)
RN  - EC 4.2.1.- (Carbonic Anhydrase II)
SB  - IM
MH  - Carbonic Anhydrase II/genetics/metabolism
MH  - Cortactin/genetics/metabolism
MH  - Down-Regulation/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Insulin-Like Growth Factor Binding Protein 4/genetics/metabolism
MH  - Lymphatic Metastasis
MH  - Neoplasm Staging/*methods
MH  - Oligonucleotide Array Sequence Analysis/*methods
MH  - Prognosis
MH  - Protein Array Analysis/*methods
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - *Stomach Neoplasms/genetics/metabolism/secondary
MH  - Up-Regulation/genetics
EDAT- 2010/06/29 06:00
MHDA- 2010/09/30 06:00
CRDT- 2010/06/29 06:00
PHST- 2010/06/29 06:00 [entrez]
PHST- 2010/06/29 06:00 [pubmed]
PHST- 2010/09/30 06:00 [medline]
AID - CDD432 [pii]
AID - 10.1111/j.1751-2980.2010.00432.x [doi]
PST - ppublish
SO  - J Dig Dis. 2010 Jun;11(3):167-75. doi: 10.1111/j.1751-2980.2010.00432.x.