PMID- 20579482 OWN - NLM STAT- MEDLINE DCOM- 20100917 LR - 20161125 IS - 1873-4324 (Electronic) IS - 0003-2670 (Linking) VI - 672 IP - 1-2 DP - 2010 Jul 5 TI - Color encoded microbeads-based flow cytometric immunoassay for polycyclic aromatic hydrocarbons in food. PG - 9-14 LID - 10.1016/j.aca.2010.05.005 [doi] AB - Food contamination caused by chemical hazards such as persistent organic pollutants (POPs) is a worldwide public health concern and requires continuous monitoring. The chromatography-based analysis methods for POPs are accurate and quite sensitive but they are time-consuming, laborious and expensive. Thus, there is a need for validated simplified screening tools, which are inexpensive, rapid, have automation potential and can detect multiple POPs simultaneously. In this study we developed a flow cytometry-based immunoassay (FCIA) using a color-encoded microbeads technology to detect benzo[a]pyrene (BaP) and other polycyclic aromatic hydrocarbons (PAHs) in buffer and food extracts as a starting point for the future development of rapid multiplex assays including other POPs in food, such as polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs). A highly sensitive assay for BaP was obtained with an IC(50) of 0.3 microg L(-1) using a monoclonal antibody (Mab22F12) against BaP, similar to the IC(50) of a previously described enzyme-linked immunosorbent assay (ELISA) using the same Mab. Moreover, the FCIA was 8 times more sensitive for BaP compared to a surface plasmon resonance (SPR)-based biosensor immunoassay (BIA) using the same reagents. The selectivity of the FCIAs was tested, with two Mabs against BaP for 25 other PAHs, including two hydroxyl PAH metabolites. Apart from BaP, the FCIAs can detect PAHs such as indenol[1,2,3-cd]pyrene (IP), benz[a]anthracene (BaA), and chrysene (CHR) which are also appointed by the European Food Safety Authority (EFSA) as suitable indicators of PAH contamination in food. The FCIAs results were in agreement with those obtained with gas chromatography-mass spectrometry (GC-MS) for the detection of PAHs in real food samples of smoked carp and wheat flour and has great potential for the future routine application of this assay in a simplex or multiplex format in combination with simplified extraction procedure which are under development. CI - Copyright 2010 Elsevier B.V. All rights reserved. FAU - Meimaridou, Anastasia AU - Meimaridou A AD - RIKILT-Institute of Food Safety, Wageningen UR, P.O. Box 230, 6700 AE Wageningen, The Netherlands. anastasia.meimaridou@wur.nl FAU - Haasnoot, Willem AU - Haasnoot W FAU - Noteboom, Linda AU - Noteboom L FAU - Mintzas, Dimitrios AU - Mintzas D FAU - Pulkrabova, Jana AU - Pulkrabova J FAU - Hajslova, Jana AU - Hajslova J FAU - Nielen, Michel W F AU - Nielen MW LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20100510 PL - Netherlands TA - Anal Chim Acta JT - Analytica chimica acta JID - 0370534 RN - 0 (Antibodies, Monoclonal) RN - 0 (Polycyclic Aromatic Hydrocarbons) RN - 3417WMA06D (Benzo(a)pyrene) SB - IM MH - Animals MH - Antibodies, Monoclonal/immunology MH - Benzo(a)pyrene/analysis MH - Carps MH - Color MH - Fish Products/analysis MH - Flour/analysis MH - Flow Cytometry/*methods MH - Food Contamination/*analysis MH - Immunoassay MH - Microspheres MH - Polycyclic Aromatic Hydrocarbons/*analysis/immunology MH - Sensitivity and Specificity MH - Triticum/chemistry EDAT- 2010/06/29 06:00 MHDA- 2010/09/21 06:00 CRDT- 2010/06/29 06:00 PHST- 2009/12/16 00:00 [received] PHST- 2010/04/27 00:00 [revised] PHST- 2010/05/02 00:00 [accepted] PHST- 2010/06/29 06:00 [entrez] PHST- 2010/06/29 06:00 [pubmed] PHST- 2010/09/21 06:00 [medline] AID - S0003-2670(10)00576-3 [pii] AID - 10.1016/j.aca.2010.05.005 [doi] PST - ppublish SO - Anal Chim Acta. 2010 Jul 5;672(1-2):9-14. doi: 10.1016/j.aca.2010.05.005. Epub 2010 May 10.