PMID- 20579639
OWN - NLM
STAT- MEDLINE
DCOM- 20110121
LR  - 20101220
IS  - 1556-5653 (Electronic)
IS  - 0015-0282 (Linking)
VI  - 95
IP  - 1
DP  - 2011 Jan
TI  - A case of severe asthenozoospermia: a novel sperm tail defect of possible genetic 
      origin identified by electron microscopy and immunocytochemistry.
PG  - 289.e11-6
LID - 10.1016/j.fertnstert.2010.05.029 [doi]
AB  - OBJECTIVE: To characterize a novel flagellar defect involving 98% of sperm tails. 
      DESIGN: Case report. SETTING: Interdepartmental Centre for Research and Therapy 
      of Male Infertility, Siena, Italy. PATIENT(S): A 45-year-old infertile man with 
      severe asthenozoospermia. INTERVENTION(S): Family history, physical examination, 
      hormonal analysis, microbial assays, semen analysis, transmission and scanning 
      electron microscopy, tubulin distribution investigated by immunocytochemistry, 
      fluorescence in situ hybridization (FISH) for chromosomes 9, 16, 18, X, and Y. 
      MAIN OUTCOME MEASURE(S): Flagellar abnormalities detected by microscopical 
      methods. RESULT(S): An apparent heterogeneity was observed: extremely elongated 
      tails prone to ruptures; coiled tails at different levels with a strongly rolled 
      axoneme or with a curl in the final flagellar segment; and V-shaped, isolated, 
      bent tails. Transmission electron microscopy revealed the presence of normal 
      heads, disorganized flagellar structures, and dynein deficiency. The FISH 
      analysis was normal. CONCLUSION(S): We report a new sperm defect, characterized 
      by abnormal elongation of the tail, which was prone to ruptures at different 
      levels, concomitant with coiled tails, which were impossible to measure in 
      length. This defect remained constant in different examined ejaculates and 
      applied to the entire sperm population of a sterile man, the son of first-degree 
      cousins, indicating a potential genetic origin.
CI  - Copyright (c) 2011 American Society for Reproductive Medicine. Published by 
      Elsevier Inc. All rights reserved.
FAU - Collodel, Giulia
AU  - Collodel G
AD  - Interdepartmental Centre for Research and Therapy of Male Infertility, Department 
      of Biomedical Sciences, Applied Biology Section, University of Siena, Siena, 
      Italy. collodel@unisi.it
FAU - Federico, Maria Grazia
AU  - Federico MG
FAU - Pascarelli, Nicola Antonio
AU  - Pascarelli NA
FAU - Geminiani, Michela
AU  - Geminiani M
FAU - Renieri, Tommaso
AU  - Renieri T
FAU - Moretti, Elena
AU  - Moretti E
LA  - eng
PT  - Case Reports
PT  - Journal Article
DEP - 20100625
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
SB  - IM
MH  - Asthenozoospermia/*genetics/*pathology
MH  - Chromosomes, Human, Pair 16
MH  - Chromosomes, Human, Pair 18
MH  - Chromosomes, Human, Pair 9
MH  - Chromosomes, Human, X
MH  - Chromosomes, Human, Y
MH  - Diploidy
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Microscopy, Electron, Scanning
MH  - Microscopy, Electron, Transmission
MH  - Middle Aged
MH  - Severity of Illness Index
MH  - Sperm Tail/*pathology/*ultrastructure
EDAT- 2010/06/29 06:00
MHDA- 2011/01/22 06:00
CRDT- 2010/06/29 06:00
PHST- 2010/03/12 00:00 [received]
PHST- 2010/04/29 00:00 [revised]
PHST- 2010/05/04 00:00 [accepted]
PHST- 2010/06/29 06:00 [entrez]
PHST- 2010/06/29 06:00 [pubmed]
PHST- 2011/01/22 06:00 [medline]
AID - S0015-0282(10)00819-8 [pii]
AID - 10.1016/j.fertnstert.2010.05.029 [doi]
PST - ppublish
SO  - Fertil Steril. 2011 Jan;95(1):289.e11-6. doi: 10.1016/j.fertnstert.2010.05.029. 
      Epub 2010 Jun 25.