PMID- 20579641
OWN - NLM
STAT- MEDLINE
DCOM- 20100920
LR  - 20200816
IS  - 1556-5653 (Electronic)
IS  - 0015-0282 (Linking)
VI  - 94
IP  - 4
DP  - 2010 Sep
TI  - The use of arrays in preimplantation genetic diagnosis and screening.
PG  - 1173-1177
LID - S0015-0282(10)00702-8 [pii]
LID - 10.1016/j.fertnstert.2010.04.064 [doi]
AB  - BACKGROUND: In preimplantation genetic diagnosis (PGD), polymerase chain reaction 
      has been used to detect monogenic disorders, and in PGD/preimplantation genetic 
      screening (PGS), fluorescence in situ hybridization (FISH) has been used to 
      analyze chromosomes. Ten randomized controlled trials (RCTs) using FISH-based PGS 
      on cleavage-stage embryos and one on blastocyst-stage embryos have shown that PGS 
      does not increase delivery rates. Is the failure of PGS due to a fundamental flaw 
      in the idea, or are the techniques that are being used unable to overcome their 
      own, inherent flaws? Array-based technology allows for analysis of all of the 
      chromosomes. Two types of arrays are being developed for use in PGD; array 
      comparative genomic hybridization (aCGH) and single nucleotide polymorphism-based 
      (SNP) arrays. Each array can determine the number of chromosomes, however, 
      SNP-based arrays can also be used to haplotype the sample. OBJECTIVE(S): To 
      describe aCGH and SNP array technology and make suggestions for the future use of 
      arrays in PGD and PGS. CONCLUSION(S): If array-based testing is going to prove 
      useful, three steps need to be taken: [1] Validation of the array platform on 
      appropriate cell and tissue samples to allow for reliable testing, even at the 
      single-cell level; [2] deciding which embryo stage is the best for biopsy: polar 
      body, cleavage, or blastocyst stage; [3] performing RCTs to show improvement in 
      delivery rates. If RCTs are able to show that array-based testing at the optimal 
      stage for embryo biopsy increases delivery rates, this will be a major step 
      forward for assisted reproductive technology patients around the world.
CI  - Copyright (c) 2010 American Society for Reproductive Medicine. Published by 
      Elsevier Inc. All rights reserved.
FAU - Harper, Joyce C
AU  - Harper JC
AD  - Reader in Human Genetics and Embryology, University College London Centre for 
      Preimplantation Genetics and Diagnosis, Institute for Women's Health, University 
      College London and Centre for Reproductive and Genetic Health, Institute for 
      Women's Health, University College London Hospital, London, United Kingdom. 
      Electronic address: joyce.harper@ucl.ac.uk.
FAU - Harton, Gary
AU  - Harton G
AD  - Preimplantation Genetic Diagnosis, Genetics & IVF Institute, Fairfax, Virginia.
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20100625
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
SB  - IM
MH  - Comparative Genomic Hybridization/methods
MH  - Female
MH  - Genetic Testing/ethics/*methods
MH  - Humans
MH  - Models, Biological
MH  - Oligonucleotide Array Sequence Analysis/ethics/*statistics & numerical data
MH  - Polymorphism, Single Nucleotide
MH  - Pregnancy
MH  - Preimplantation Diagnosis/ethics/*methods/trends
RF  - 45
EDAT- 2010/06/29 06:00
MHDA- 2010/09/21 06:00
CRDT- 2010/06/29 06:00
PHST- 2009/11/11 00:00 [received]
PHST- 2010/04/20 00:00 [revised]
PHST- 2010/04/26 00:00 [accepted]
PHST- 2010/06/29 06:00 [entrez]
PHST- 2010/06/29 06:00 [pubmed]
PHST- 2010/09/21 06:00 [medline]
AID - S0015-0282(10)00702-8 [pii]
AID - 10.1016/j.fertnstert.2010.04.064 [doi]
PST - ppublish
SO  - Fertil Steril. 2010 Sep;94(4):1173-1177. doi: 10.1016/j.fertnstert.2010.04.064. 
      Epub 2010 Jun 25.