PMID- 20579763
OWN - NLM
STAT- MEDLINE
DCOM- 20110311
LR  - 20100809
IS  - 1878-3279 (Electronic)
IS  - 0171-2985 (Linking)
VI  - 215
IP  - 9-10
DP  - 2010 Sep-Oct
TI  - Lower SCD expression in dendritic cells compared to macrophages leads to membrane 
      lipids with less mono-unsaturated fatty acids.
PG  - 748-55
LID - 10.1016/j.imbio.2010.05.016 [doi]
AB  - Macrophages and dendritic cells originate from a common myeloid precursor. 
      Although several studies compared transcriptional profiles of these cells, not a 
      single study compared their lipid profiles. Therefore, we measured and compared 
      fatty acid (FA) and phospholipid (PL) species composition of 
      granulocyte/macrophage colony-stimulating factor (GM-CSF) plus interleukin 4 
      (IL-4) and macrophage colony-stimulating factor (M-CSF) differentiated monocytes 
      isolated from healthy volunteers. We found that these two cell types prominently 
      differ in their FA composition. Dendritic cells (DCs) contain lower proportions 
      of C16 and C18 mono-unsaturated FAs, but higher proportions of C20 and C22 
      poly-unsaturated fatty acids (PUFAs) than phagocytic macrophages. Analysis of PL 
      species profiles revealed that GM-CSF/IL-4 differentiated cells have increased 
      amounts of longer and more desaturated phospatidylethanolamine (PE) and 
      phosphatidylserine (PS) species, but lower amounts of shorter and less 
      desaturated PE and PS species than M-CSF differentiated cells. These cell type 
      specific lipid profiles can be attributed to a differential expression and 
      activity of stearoyl-CoA desaturase (SCD). Taken together, our results show that 
      GM-CSF/IL-4 compared to M-CSF differentiated monocytes have less mono-unsaturated 
      FA and PL species, which are due to lower SCD activity observed in DCs.
CI  - Copyright 2010 Elsevier GmbH. All rights reserved.
FAU - Ecker, Josef
AU  - Ecker J
AD  - Institute of Clinical Chemistry and Laboratory Medicine, University of 
      Regensburg, Regensburg Germany.
FAU - Liebisch, Gerhard
AU  - Liebisch G
FAU - Grandl, Margot
AU  - Grandl M
FAU - Schmitz, Gerd
AU  - Schmitz G
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100604
PL  - Netherlands
TA  - Immunobiology
JT  - Immunobiology
JID - 8002742
RN  - 0 (Fatty Acids, Monounsaturated)
RN  - 0 (Phospholipids)
RN  - 207137-56-2 (Interleukin-4)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - EC 1.14.19.1 (Stearoyl-CoA Desaturase)
SB  - IM
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Dendritic Cells/*metabolism/pathology
MH  - Fatty Acids, Monounsaturated/*metabolism
MH  - Gene Expression Regulation
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/metabolism
MH  - Humans
MH  - Interleukin-4/metabolism
MH  - Macrophage Colony-Stimulating Factor/metabolism
MH  - Macrophages/*metabolism/pathology
MH  - Monocytes/cytology
MH  - Phagocytes/*metabolism/pathology
MH  - Phospholipids/metabolism
MH  - Stearoyl-CoA Desaturase/*metabolism
EDAT- 2010/06/29 06:00
MHDA- 2011/03/12 06:00
CRDT- 2010/06/29 06:00
PHST- 2010/04/21 00:00 [received]
PHST- 2010/05/20 00:00 [accepted]
PHST- 2010/06/29 06:00 [entrez]
PHST- 2010/06/29 06:00 [pubmed]
PHST- 2011/03/12 06:00 [medline]
AID - S0171-2985(10)00082-3 [pii]
AID - 10.1016/j.imbio.2010.05.016 [doi]
PST - ppublish
SO  - Immunobiology. 2010 Sep-Oct;215(9-10):748-55. doi: 10.1016/j.imbio.2010.05.016. 
      Epub 2010 Jun 4.