PMID- 20580309
OWN - NLM
STAT- MEDLINE
DCOM- 20101014
LR  - 20141120
IS  - 1873-5967 (Electronic)
IS  - 1386-6532 (Linking)
VI  - 48
IP  - 4
DP  - 2010 Aug
TI  - Acute hepatitis B infection associated with drug-resistant hepatitis B virus.
PG  - 270-4
LID - 10.1016/j.jcv.2010.05.010 [doi]
AB  - BACKGROUND: Pre-existing antiviral-resistant hepatitis B virus (HBV) has been 
      associated with primary non-response to lamivudine treatment in patients with 
      chronic hepatitis B, but little is known of the capacity for resistant HBV to 
      cause primary infection. OBJECTIVE: The study was to investigate if Beijing 
      patients with acute hepatitis B (AHB) are infected with drug-resistant HBV. STUDY 
      DESIGN: Sera were collected from 201 NA-untreated patients with AHB. Direct 
      polymerase chain reaction (PCR) sequencing was used to screen HBV 
      reverse-transcriptase (RT) domain and clonal sequencing were performed for all 
      resistance-positive samples. RESULTS: Direct PCR sequencing showed that 14 
      samples (7.0%) were positive for drug-resistant HBV variants, comprised of 11 
      with the lamivudine-resistant pattern rtM204I and/or rtM204V in the presence and 
      absence of compensatory mutations rtL80I, rtV173L, and rtL180M; two with the 
      adefovir-resistant pattern rtA181V; and one with the entecavir-resistant pattern 
      rtL180M+rtS202G+rtM204V. Concomitance of resistance variants with wild-type HBV 
      was observed in samples from 13 patients. Clonal sequencing verified direct 
      sequencing results. Furthermore, variants associated with resistance to adefovir 
      or entecavir were found in 3 samples. CONCLUSIONS: Drug-resistant HBV strains, 
      including those not resistant to lamivudine, are transmissible and can cause 
      acute hepatitis B in China.
CI  - Copyright 2010 Elsevier B.V. All rights reserved.
FAU - Xu, Zhihui
AU  - Xu Z
AD  - Viral Hepatitis Research Laboratory, Institute of Infectious Diseases, Beijing 
      302 Hospital, 100 Xi Si Huan Middle Road, Beijing 100039, China.
FAU - Liu, Yan
AU  - Liu Y
FAU - Xu, Teng
AU  - Xu T
FAU - Chen, Li
AU  - Chen L
FAU - Si, Lanlan
AU  - Si L
FAU - Wang, Yao
AU  - Wang Y
FAU - Ren, Xiaoqiang
AU  - Ren X
FAU - Zhong, Yanwei
AU  - Zhong Y
FAU - Zhao, Jingmin
AU  - Zhao J
FAU - Xu, Dongping
AU  - Xu D
LA  - eng
SI  - GENBANK/GQ377514
SI  - GENBANK/GQ377515
SI  - GENBANK/GQ377516
SI  - GENBANK/GQ377517
SI  - GENBANK/GQ377518
SI  - GENBANK/GQ377519
SI  - GENBANK/GQ377520
SI  - GENBANK/GQ377521
SI  - GENBANK/GQ377522
SI  - GENBANK/GQ377523
SI  - GENBANK/GQ377524
SI  - GENBANK/GQ377525
SI  - GENBANK/GQ377526
SI  - GENBANK/GQ377527
SI  - GENBANK/GQ377528
SI  - GENBANK/GQ377529
SI  - GENBANK/GQ377530
SI  - GENBANK/GQ377531
SI  - GENBANK/GQ377532
SI  - GENBANK/GQ377533
SI  - GENBANK/GQ377534
SI  - GENBANK/GQ377535
SI  - GENBANK/GQ377536
SI  - GENBANK/GQ377537
SI  - GENBANK/GQ377538
SI  - GENBANK/GQ377539
SI  - GENBANK/GQ377540
SI  - GENBANK/GQ377541
SI  - GENBANK/GQ377542
SI  - GENBANK/GQ377543
SI  - GENBANK/GQ377544
SI  - GENBANK/GQ377545
SI  - GENBANK/GQ377546
SI  - GENBANK/GQ377547
SI  - GENBANK/GQ377548
SI  - GENBANK/GQ377549
SI  - GENBANK/GQ377550
SI  - GENBANK/GQ377551
SI  - GENBANK/GQ377552
SI  - GENBANK/GQ377553
SI  - GENBANK/GQ377554
SI  - GENBANK/GQ377555
SI  - GENBANK/GQ377556
SI  - GENBANK/GQ377557
SI  - GENBANK/GQ377558
SI  - GENBANK/GQ377559
SI  - GENBANK/GQ377560
SI  - GENBANK/GQ377561
SI  - GENBANK/GQ377562
SI  - GENBANK/GQ377563
SI  - GENBANK/GQ377564
SI  - GENBANK/GQ377565
SI  - GENBANK/GQ377566
SI  - GENBANK/GQ377567
SI  - GENBANK/GQ377568
SI  - GENBANK/GQ377569
SI  - GENBANK/GQ377570
SI  - GENBANK/GQ377571
SI  - GENBANK/GQ377572
SI  - GENBANK/GQ377573
SI  - GENBANK/GQ377574
SI  - GENBANK/GQ377575
SI  - GENBANK/GQ377576
SI  - GENBANK/GQ377577
SI  - GENBANK/GQ377578
SI  - GENBANK/GQ377579
SI  - GENBANK/GQ377580
SI  - GENBANK/GQ377581
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SI  - GENBANK/GQ377584
SI  - GENBANK/GQ377585
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SI  - GENBANK/GQ377588
SI  - GENBANK/GQ377589
SI  - GENBANK/GQ377590
SI  - GENBANK/GQ377591
SI  - GENBANK/GQ377592
SI  - GENBANK/GQ377593
SI  - GENBANK/GQ377594
SI  - GENBANK/GQ377595
SI  - GENBANK/GQ377596
SI  - GENBANK/GQ377597
SI  - GENBANK/GQ377598
SI  - GENBANK/GQ377599
SI  - GENBANK/GQ377600
SI  - GENBANK/GQ377601
SI  - GENBANK/GQ377602
SI  - GENBANK/GQ377603
SI  - GENBANK/GQ377604
SI  - GENBANK/GQ377605
SI  - GENBANK/GQ377606
SI  - GENBANK/GQ377607
SI  - GENBANK/GQ377608
SI  - GENBANK/GQ377609
SI  - GENBANK/GQ377610
SI  - GENBANK/GQ377611
SI  - GENBANK/GQ377612
SI  - GENBANK/GQ377613
SI  - GENBANK/GQ377614
SI  - GENBANK/GQ377615
SI  - GENBANK/GQ377616
SI  - GENBANK/GQ377617
SI  - GENBANK/GQ377618
SI  - GENBANK/GQ377619
SI  - GENBANK/GQ377620
SI  - GENBANK/GQ377621
SI  - GENBANK/GQ377622
SI  - GENBANK/GQ377623
SI  - GENBANK/GQ377624
SI  - GENBANK/GQ377625
SI  - GENBANK/GQ377626
SI  - GENBANK/GQ377627
SI  - GENBANK/GQ377628
SI  - GENBANK/GQ377629
SI  - GENBANK/GQ377630
SI  - GENBANK/GQ377631
SI  - GENBANK/GQ377632
SI  - GENBANK/GQ377633
SI  - GENBANK/GQ377634
SI  - GENBANK/GQ377635
SI  - GENBANK/GQ377636
SI  - GENBANK/GQ377637
SI  - GENBANK/GQ377638
SI  - GENBANK/GQ377639
SI  - GENBANK/GQ377640
SI  - GENBANK/GQ377641
SI  - GENBANK/GQ377642
SI  - GENBANK/GQ377643
SI  - GENBANK/GQ377644
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100626
PL  - Netherlands
TA  - J Clin Virol
JT  - Journal of clinical virology : the official publication of the Pan American 
      Society for Clinical Virology
JID - 9815671
RN  - 0 (Antiviral Agents)
RN  - 0 (DNA, Viral)
RN  - 0 (Organophosphonates)
RN  - 0 (Viral Proteins)
RN  - 2T8Q726O95 (Lamivudine)
RN  - 5968Y6H45M (entecavir)
RN  - 5Z93L87A1R (Guanine)
RN  - 6GQP90I798 (adefovir)
RN  - EC 2.7.7.49 (RNA-Directed DNA Polymerase)
RN  - JAC85A2161 (Adenine)
SB  - IM
MH  - Adenine/analogs & derivatives/pharmacology
MH  - Adult
MH  - Amino Acid Substitution/genetics
MH  - Antiviral Agents/*pharmacology
MH  - China
MH  - DNA, Viral/chemistry/genetics
MH  - *Drug Resistance, Viral
MH  - Guanine/analogs & derivatives/pharmacology
MH  - Hepatitis B/*virology
MH  - Hepatitis B virus/*drug effects/*isolation & purification
MH  - Humans
MH  - Lamivudine/pharmacology
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - *Mutation, Missense
MH  - Organophosphonates/pharmacology
MH  - Polymerase Chain Reaction
MH  - RNA-Directed DNA Polymerase/genetics
MH  - Sequence Analysis, DNA
MH  - Viral Proteins/genetics
EDAT- 2010/06/29 06:00
MHDA- 2010/10/15 06:00
CRDT- 2010/06/29 06:00
PHST- 2010/03/17 00:00 [received]
PHST- 2010/05/18 00:00 [revised]
PHST- 2010/05/25 00:00 [accepted]
PHST- 2010/06/29 06:00 [entrez]
PHST- 2010/06/29 06:00 [pubmed]
PHST- 2010/10/15 06:00 [medline]
AID - S1386-6532(10)00223-4 [pii]
AID - 10.1016/j.jcv.2010.05.010 [doi]
PST - ppublish
SO  - J Clin Virol. 2010 Aug;48(4):270-4. doi: 10.1016/j.jcv.2010.05.010. Epub 2010 Jun 
      26.