PMID- 20581009
OWN - NLM
STAT- MEDLINE
DCOM- 20110210
LR  - 20220330
IS  - 1460-2423 (Electronic)
IS  - 0959-6658 (Linking)
VI  - 20
IP  - 11
DP  - 2010 Nov
TI  - Characterization and binding activity of the chondroitin/dermatan sulfate chain 
      from Endocan, a soluble endothelial proteoglycan.
PG  - 1380-8
LID - 10.1093/glycob/cwq100 [doi]
AB  - Endocan is a recently identified soluble chondroitin/dermatan sulfate (CS/DS) 
      proteoglycan. Synthesized by endothelial cells, it has been found to be 
      over-expressed in the vasculature surrounding a number of tumors, and by 
      promoting growth factor mitogenic activities, hepatocyte growth factor/scatter 
      factor (HGF/SF) in particular, it supports cellular proliferation. In this work, 
      we characterized the glycosaminoglycan (GAG) chain of Endocan, purified either 
      from the naturally producing human umbilical vein endothelial cells (HUVEC) or 
      from a recombinant over-expression system in human embryonic kidney cells (HEK). 
      Compositional analysis using different chondroitinases as well as nuclear 
      magnetic resonance studies revealed that the GAG chains from both sources share 
      many characteristics, with the exception of size (15 and 40 kDa, respectively, 
      for HUVEC and HEK-293 cells). The DS-specific, IdoA-containing disaccharides 
      contribute 30% of the chain (15% of which are 2-O-sulfated) and are mostly 
      clustered in tetra- (35%), hexa- (12%), and octa- (5%) saccharide domains. Highly 
      sulfated D, E, and B disaccharide units (HexA2S-GalNAc6S, HexA-GalNAc4S6S, and 
      HexA2S-GalNAc4S) were also detected in significant amounts in both chains and may 
      account for the HGF/SF-binding activity of the CS/DS. This work establishes that 
      HEK-293 cells can be engineered to provide a valuable source of Endocan with 
      authentic CS/DS chains, enabling the purification of sufficient amounts for 
      structural and/or binding analysis and providing a possible model of Endocan 
      CS/DS chain organization.
FAU - Sarrazin, Stephane
AU  - Sarrazin S
AD  - Institut de Biologie Structurale, UMR 5075 CNRS-CEA-UJF, 41 Rue Horowitz, 38027 
      Grenoble, France.
FAU - Lyon, Malcolm
AU  - Lyon M
FAU - Deakin, Jon A
AU  - Deakin JA
FAU - Guerrini, Marco
AU  - Guerrini M
FAU - Lassalle, Philippe
AU  - Lassalle P
FAU - Delehedde, Maryse
AU  - Delehedde M
FAU - Lortat-Jacob, Hugues
AU  - Lortat-Jacob H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100624
PL  - England
TA  - Glycobiology
JT  - Glycobiology
JID - 9104124
RN  - 0 (Proteoglycans)
RN  - 24967-94-0 (Dermatan Sulfate)
RN  - 9007-27-6 (Chondroitin)
SB  - IM
MH  - Binding Sites
MH  - Cells, Cultured
MH  - Chondroitin/*metabolism
MH  - Chromatography, Gel
MH  - Dermatan Sulfate/*metabolism
MH  - Humans
MH  - Magnetic Resonance Spectroscopy
MH  - Proteoglycans/*metabolism
EDAT- 2010/06/29 06:00
MHDA- 2011/02/11 06:00
CRDT- 2010/06/29 06:00
PHST- 2010/06/29 06:00 [entrez]
PHST- 2010/06/29 06:00 [pubmed]
PHST- 2011/02/11 06:00 [medline]
AID - cwq100 [pii]
AID - 10.1093/glycob/cwq100 [doi]
PST - ppublish
SO  - Glycobiology. 2010 Nov;20(11):1380-8. doi: 10.1093/glycob/cwq100. Epub 2010 Jun 
      24.