PMID- 20582699
OWN - NLM
STAT- MEDLINE
DCOM- 20101116
LR  - 20160512
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 398
IP  - 1
DP  - 2010 Sep
TI  - Exploratory analysis of human urine by LC-ESI-TOF MS after high intake of olive 
      oil: understanding the metabolism of polyphenols.
PG  - 463-75
LID - 10.1007/s00216-010-3899-x [doi]
AB  - Olive oil polyphenols have important biological properties which closely depend 
      on their bioavailability; it is, therefore, essential to understand how 
      polyphenols are absorbed, metabolized, and eliminated from the body. An 
      analytical method based on rapid-resolution liquid chromatography (RRLC) coupled 
      with mass spectrometric detection with a time-of-flight analyzer (RRLC-ESI-TOF 
      MS) has been developed for analysis of the main olive oil phenolic compounds and 
      their metabolites in human urine. Urine samples from ten healthy volunteers were 
      collected before and 2, 4, and 6 h after intake of 50 mL extra-virgin olive oil. 
      The proposed method includes liquid-liquid extraction with ethyl acetate, which 
      provides extraction recoveries of the phenolic compounds studied between 35 and 
      75% from spiked urine samples. Good repeatability was obtained--the relative 
      standard deviations (RSDs) of peak areas in intra-day and inter-day studies were 
      4.3 and 6.5%, respectively. Statistical studies enabled us to discriminate 
      between urine samples before and after intake, and facilitated the search for m/z 
      values enabling this discrimination. Based on the very accurate mass information 
      and the isotopic pattern provided by the TOF MS analyzer, together with other 
      available information, ten of these biomarkers and more than 50 metabolites, 
      obtained through phase I and phase II biotransformation reactions, were 
      tentatively identified. Additionally, kinetic studies were conducted on the 
      metabolites identified as possible biomarkers; for most of the compounds 
      concentrations were maximum in the first two hours.
FAU - Garcia-Villalba, Rocio
AU  - Garcia-Villalba R
AD  - Research Group FQM-297, Department of Analytical Chemistry, Faculty of Sciences, 
      University of Granada, C/Fuentenueva s/n, 18071 Granada, Spain.
FAU - Carrasco-Pancorbo, Alegria
AU  - Carrasco-Pancorbo A
FAU - Nevedomskaya, Ekaterina
AU  - Nevedomskaya E
FAU - Mayboroda, Oleg A
AU  - Mayboroda OA
FAU - Deelder, Andre M
AU  - Deelder AM
FAU - Segura-Carretero, Antonio
AU  - Segura-Carretero A
FAU - Fernandez-Gutierrez, Alberto
AU  - Fernandez-Gutierrez A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100627
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Biomarkers)
RN  - 0 (Flavonoids)
RN  - 0 (Olive Oil)
RN  - 0 (Phenols)
RN  - 0 (Plant Oils)
RN  - 0 (Polyphenols)
SB  - IM
MH  - Adult
MH  - Biomarkers/*analysis
MH  - *Chromatography, Liquid
MH  - Female
MH  - Flavonoids/*urine
MH  - Humans
MH  - Kinetics
MH  - Male
MH  - Olive Oil
MH  - Phenols/*urine
MH  - Plant Oils/*chemistry
MH  - Polyphenols
MH  - *Spectrometry, Mass, Electrospray Ionization
MH  - *Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Young Adult
EDAT- 2010/06/29 06:00
MHDA- 2010/11/17 06:00
CRDT- 2010/06/29 06:00
PHST- 2010/02/11 00:00 [received]
PHST- 2010/06/06 00:00 [accepted]
PHST- 2010/06/01 00:00 [revised]
PHST- 2010/06/29 06:00 [entrez]
PHST- 2010/06/29 06:00 [pubmed]
PHST- 2010/11/17 06:00 [medline]
AID - 10.1007/s00216-010-3899-x [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2010 Sep;398(1):463-75. doi: 10.1007/s00216-010-3899-x. Epub 
      2010 Jun 27.