PMID- 20583317
OWN - NLM
STAT- MEDLINE
DCOM- 20101110
LR  - 20100728
IS  - 1099-1263 (Electronic)
IS  - 0260-437X (Linking)
VI  - 30
IP  - 5
DP  - 2010 Jul
TI  - Skin sensitization, false positives and false negatives: experience with guinea 
      pig assays.
PG  - 381-6
LID - 10.1002/jat.1545 [doi]
AB  - The advent of the local lymph node assay (LLNA), and efforts to develop in vitro 
      alternatives for the identification of skin sensitizing chemicals has focused 
      attention on the issue of false positive and false negative results. In essence, 
      the question becomes 'what is the gold standard?' In this context, attention has 
      focused primarily on the LLNA as this is now the preferred assay for skin 
      sensitization testing. However, for many years prior to introduction of the LLNA, 
      the guinea pig maximization test and the occluded patch test of Buehler were the 
      methods of choice. In order to encourage a more informed dialogue about the 
      relative performance, accuracy and applicability of the LLNA and guinea pig 
      tests, we have here considered the extent to which guinea pig methods were 
      themselves subject to false positives and negative results. We describe and 
      discuss here well-characterized examples of instances where both false negatives 
      (including abietic acid and eugenol) or false positives (including vanillin and 
      sulfanilic acid) have been recorded in guinea pig tests. These and other examples 
      are discussed with particular reference to the fabrication of a gold standard 
      dataset that is required for the validation of in vitro alternatives.
CI  - Copyright (c) 2010 John Wiley & Sons, Ltd.
FAU - Basketter, David A
AU  - Basketter DA
AD  - DABMEB Consultancy Ltd, Sharnbrook, UK. david.basketter@ukonline.co.uk
FAU - Kimber, Ian
AU  - Kimber I
LA  - eng
PT  - Journal Article
PT  - Review
PL  - England
TA  - J Appl Toxicol
JT  - Journal of applied toxicology : JAT
JID - 8109495
RN  - 0 (Allergens)
SB  - IM
MH  - Allergens/classification/*toxicity
MH  - Animal Testing Alternatives
MH  - Animals
MH  - Cells, Cultured
MH  - Cricetinae
MH  - Dermatitis, Contact/*etiology
MH  - False Negative Reactions
MH  - False Positive Reactions
MH  - Humans
MH  - *Local Lymph Node Assay
MH  - Lymph Nodes/drug effects
MH  - *Patch Tests
MH  - Predictive Value of Tests
MH  - Skin/*drug effects
MH  - Toxicity Tests/*methods
EDAT- 2010/06/29 06:00
MHDA- 2010/11/11 06:00
CRDT- 2010/06/29 06:00
PHST- 2010/06/29 06:00 [entrez]
PHST- 2010/06/29 06:00 [pubmed]
PHST- 2010/11/11 06:00 [medline]
AID - 10.1002/jat.1545 [doi]
PST - ppublish
SO  - J Appl Toxicol. 2010 Jul;30(5):381-6. doi: 10.1002/jat.1545.