PMID- 20584675
OWN - NLM
STAT- MEDLINE
DCOM- 20111122
LR  - 20191210
IS  - 1673-4254 (Print)
IS  - 1673-4254 (Linking)
VI  - 30
IP  - 6
DP  - 2010 Jun
TI  - [Association of HLA-DR4, PAD4, and STAT4 expression in the peripheral blood with 
      disease activity in patients with rheumatoid arthritis].
PG  - 1349-53
AB  - OBJECTIVE: To explore the association of the expressions of human leukocyte 
      antigen (HLA)-DR4, peptidyl arginine deiminase type4(PAD4), and signal transducer 
      and activator of transcription 4 (STAT4) in the peripheral blood with the disease 
      activity in patients with rheumatoid arthritis (RA). METHODS: Twenty-four RA 
      patients in active stage (DAS28 score>or=2.6) and 14 RA patients in remission 
      stage (DAS28 score<2.6) were enrolled in this study, with 12 healthy volunteers 
      as the control. The QuantiGene Plex method was used to measure the expression 
      level of HLA-DR4, PAD4, and STAT4 mRNA, and the relationship between the 
      expressions of these genes and the DAS28 score, levels of anti-cyclic 
      citrullinated peptide antibody (anti-CCP antibody) and rheumatoid factor (RF) was 
      analyzed. RESULTS: The expressions of HLA-DR4, PAD4, and STAT4 were significantly 
      higher in RA patients than in the healthy controls (P<0.05). The level of HLA-DR4 
      mRNA in the two RA groups showed no significant difference, but was significantly 
      higher than that in the healthy controls. HLA-DR4 expression was not found to 
      correlated to DAS28 score, anti-CCP antibody level or RF in the RA patients. The 
      expressions of PAD4 and STAT4 were significantly different between the two RA 
      groups (P<0.05). In the RA patients, PAD4 mRNA expression was positively 
      correlated to DAS28 and anti-CCP antibody level (P<0.05), and STAT4 expression 
      showed positive correlations to DAS28 and RF levels (P<0.05). CONCLUSION: 
      HLA-DR4, PAD4 and STAT4 are overexpressed in RA patients and may be involved in 
      the pathogenesis of RA. The expressions of PAD4 and STAT4, but not HLA-DR4, are 
      closely related to the disease activity of RA. Detection of peripheral blood PAD4 
      and STAT4 expressions can be helpful for evaluating the disease activity of RA.
FAU - Lu, Zhuo
AU  - Lu Z
AD  - Department of Traditional Chinese Rheumatology, Nanfang Hospital, Southern 
      Medical University, Guangzhou 510515, China. zhuozhuotu@163.com
FAU - Li, Juan
AU  - Li J
FAU - Feng, Zhi-Tao
AU  - Feng ZT
FAU - Ren, Jie
AU  - Ren J
LA  - chi
PT  - Journal Article
PL  - China
TA  - Nan Fang Yi Ke Da Xue Xue Bao
JT  - Nan fang yi ke da xue xue bao = Journal of Southern Medical University
JID - 101266132
RN  - 0 (HLA-DR4 Antigen)
RN  - 0 (RNA, Messenger)
RN  - 0 (STAT4 Transcription Factor)
RN  - 0 (STAT4 protein, human)
RN  - EC 3.- (Hydrolases)
RN  - EC 3.5.3.15 (PADI4 protein, human)
RN  - EC 3.5.3.15 (Protein-Arginine Deiminase Type 4)
RN  - EC 3.5.3.15 (Protein-Arginine Deiminases)
SB  - IM
MH  - Adult
MH  - Arthritis, Rheumatoid/*blood
MH  - Female
MH  - HLA-DR4 Antigen/blood/genetics/*metabolism
MH  - Humans
MH  - Hydrolases/blood/genetics/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Protein-Arginine Deiminase Type 4
MH  - Protein-Arginine Deiminases
MH  - RNA, Messenger/genetics/metabolism
MH  - STAT4 Transcription Factor/blood/genetics/*metabolism
EDAT- 2010/06/30 06:00
MHDA- 2011/12/13 00:00
CRDT- 2010/06/30 06:00
PHST- 2010/06/30 06:00 [entrez]
PHST- 2010/06/30 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
PST - ppublish
SO  - Nan Fang Yi Ke Da Xue Xue Bao. 2010 Jun;30(6):1349-53.