PMID- 20586818
OWN - NLM
STAT- MEDLINE
DCOM- 20101217
LR  - 20211020
IS  - 1365-2613 (Electronic)
IS  - 0959-9673 (Print)
IS  - 0959-9673 (Linking)
VI  - 91
IP  - 5
DP  - 2010 Oct
TI  - Thyrocyte interleukin-18 expression is up-regulated by interferon-gamma and may 
      contribute to thyroid destruction in Hashimoto's thyroiditis.
PG  - 420-5
LID - 10.1111/j.1365-2613.2010.00715.x [doi]
AB  - Interferon-gamma (IFN-gamma) has a direct role in thyroid destruction in autoimmune 
      thyroiditis. Interleukin-18 (IL-18), a pro-inflammatory cytokine with potent 
      IFN-gamma inducing activities, may play an important role in Th1-mediated autoimmune 
      diseases. The purpose of this study was to characterize the expression and 
      localization of IL-18 in the thyroid tissues of Hashimoto's thyroiditis (HT) and 
      to investigate the effect of IFN-gamma on IL-18 expression in isolated human thyroid 
      follicular cells (TFCs). Thyroid tissues obtained from six euthyroid patients 
      with HT and six control subjects were used to detect IL-18 expression by reverse 
      transcription-polymerase chain reaction (RT-PCR) and immunohistochemical 
      staining. Human TFCs were isolated and incubated for 48 h with or without IFN-gamma, 
      tumour necrosis factor-alpha (TNF-alpha) or IL-1beta. IL-18 expression was analysed by 
      RT-PCR, immunofluorescent double staining and western blot. We found that IL-18 
      expression was increased in the thyroid tissues of HT compared with control 
      thyroid tissues. TFCs were major cell types expressing IL-18 in the thyroid 
      tissues of HT. IL-18 was constitutively expressed in isolated human TFCs, and the 
      expression was significantly up-regulated by IFN-gamma rather than TNF-alpha or IL-1beta. 
      Western bolt revealed that a 24-kDa band corresponding to pro-IL-18 was broadened 
      in the lysates of IFN-gamma-treated TFCs. Our results demonstrated that IL-18 
      expression is up-regulated in the TFCs of HT patients and in primary human TFCs 
      exposed to IFN-gamma. Therefore, intrathyroidal interaction between IL-18 and IFN-gamma 
      may have a role in promoting the local immune response, which contributes to the 
      thyroid destruction seen in HT.
CI  - (c) 2010 The Authors. Journal compilation (c) 2010 Blackwell Publishing Ltd.
FAU - Liu, Zhe
AU  - Liu Z
AD  - Department of Endocrinology and Metabolism, Peking University Third Hospital, 
      Beijing, China.
FAU - Wang, Haining
AU  - Wang H
FAU - Xiao, Wenhua
AU  - Xiao W
FAU - Wang, Chen
AU  - Wang C
FAU - Liu, Guoqiang
AU  - Liu G
FAU - Hong, Tianpei
AU  - Hong T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Exp Pathol
JT  - International journal of experimental pathology
JID - 9014042
RN  - 0 (Interleukin-18)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adult
MH  - Biopsy
MH  - Cells, Cultured
MH  - Female
MH  - Gene Expression/immunology
MH  - Hashimoto Disease/immunology/metabolism/*pathology
MH  - Humans
MH  - Interferon-gamma/*metabolism
MH  - Interleukin-18/*genetics/*metabolism
MH  - Interleukin-1beta/metabolism
MH  - Male
MH  - Middle Aged
MH  - Thyroid Gland/immunology/metabolism/*pathology
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Up-Regulation/immunology
PMC - PMC3003839
EDAT- 2010/07/01 06:00
MHDA- 2010/12/18 06:00
PMCR- 2011/10/01
CRDT- 2010/07/01 06:00
PHST- 2010/07/01 06:00 [entrez]
PHST- 2010/07/01 06:00 [pubmed]
PHST- 2010/12/18 06:00 [medline]
PHST- 2011/10/01 00:00 [pmc-release]
AID - IEP715 [pii]
AID - 10.1111/j.1365-2613.2010.00715.x [doi]
PST - ppublish
SO  - Int J Exp Pathol. 2010 Oct;91(5):420-5. doi: 10.1111/j.1365-2613.2010.00715.x.