PMID- 20586938
OWN - NLM
STAT- MEDLINE
DCOM- 20101102
LR  - 20101118
IS  - 1472-765X (Electronic)
IS  - 0266-8254 (Linking)
VI  - 51
IP  - 2
DP  - 2010 Aug
TI  - High-density polyethylene (HDPE)-degrading potential bacteria from marine 
      ecosystem of Gulf of Mannar, India.
PG  - 205-11
LID - 10.1111/j.1472-765X.2010.02883.x [doi]
AB  - AIMS: Assessment of high-density polyethylene (HDPE)-degrading bacteria isolated 
      from plastic waste dumpsites of Gulf of Mannar. METHODS AND RESULTS: Rationally, 
      15 bacteria (GMB1-GMB15) were isolated by enrichment technique. GMB5 and GMB7 
      were selected for further studies based on their efficiency to degrade the HDPE 
      and identified as Arthrobacter sp. and Pseudomonas sp., respectively. Assessed 
      weight loss of HDPE after 30 days of incubation was nearly 12% for Arthrobacter 
      sp. and 15% for Pseudomonas sp. The bacterial adhesion to hydrocarbon (BATH) 
      assay showed that the cell surface hydrophobicity of Pseudomonas sp. was higher 
      than Arthrobacter sp. Both fluorescein diacetate hydrolysis and protein content 
      of the biofilm were used to test the viability and protein density of the 
      biomass. Acute peak elevation was observed between 2 and 5 days of inoculation 
      for both bacteria. Fourier transform infrared (FT-IR) spectrum showed that keto 
      carbonyl bond index (KCBI), Ester carbonyl bond index (ECBI) and Vinyl bond index 
      (VBI) were increased indicating changes in functional group(s) and/or side chain 
      modification confirming the biodegradation. CONCLUSION: The results pose us to 
      suggest that both Pseudomonas sp. and Arthrobacter sp. were proven efficient to 
      degrade HDPE, albeit the former was more efficacious, yet the ability of latter 
      cannot be neglected. SIGNIFICANCE AND IMPACT OF THE STUDY: Recent alarm on 
      ecological threats to marine system is dumping plastic waste in the marine 
      ecosystem and coastal arena by anthropogenic activity. In maintenance phase of 
      the plastic-derived polyethylene waste, the microbial degradation plays a major 
      role; the information accomplished in this work will be the initiating point for 
      the degradation of polyethylene by indigenous bacterial population in the marine 
      ecosystem and provides a novel eco-friendly solution in eco-management.
FAU - Balasubramanian, V
AU  - Balasubramanian V
AD  - Rhizosphere Biology Laboratory, Department of Microbiology, School of Life 
      Sciences, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India.
FAU - Natarajan, K
AU  - Natarajan K
FAU - Hemambika, B
AU  - Hemambika B
FAU - Ramesh, N
AU  - Ramesh N
FAU - Sumathi, C S
AU  - Sumathi CS
FAU - Kottaimuthu, R
AU  - Kottaimuthu R
FAU - Rajesh Kannan, V
AU  - Rajesh Kannan V
LA  - eng
PT  - Journal Article
DEP - 20100607
PL  - England
TA  - Lett Appl Microbiol
JT  - Letters in applied microbiology
JID - 8510094
RN  - 9002-88-4 (Polyethylene)
SB  - IM
MH  - Arthrobacter/classification/isolation & purification/*metabolism
MH  - Bacterial Adhesion
MH  - Biofilms/growth & development
MH  - Biomass
MH  - *Biotransformation
MH  - Cell Membrane/chemistry
MH  - Cell Wall/chemistry
MH  - Humans
MH  - Hydrophobic and Hydrophilic Interactions
MH  - India
MH  - Microbial Viability
MH  - Polyethylene/*metabolism
MH  - Pseudomonas/classification/isolation & purification/*metabolism
MH  - *Soil Microbiology
MH  - Spectroscopy, Fourier Transform Infrared
MH  - Time Factors
EDAT- 2010/07/01 06:00
MHDA- 2010/11/03 06:00
CRDT- 2010/07/01 06:00
PHST- 2010/07/01 06:00 [entrez]
PHST- 2010/07/01 06:00 [pubmed]
PHST- 2010/11/03 06:00 [medline]
AID - LAM2883 [pii]
AID - 10.1111/j.1472-765X.2010.02883.x [doi]
PST - ppublish
SO  - Lett Appl Microbiol. 2010 Aug;51(2):205-11. doi: 
      10.1111/j.1472-765X.2010.02883.x. Epub 2010 Jun 7.