PMID- 20591217
OWN - NLM
STAT- MEDLINE
DCOM- 20120109
LR  - 20210617
IS  - 1000-467X (Print)
IS  - 1944-446X (Linking)
VI  - 29
IP  - 7
DP  - 2010 Jul
TI  - A novel sesquiterpene Hirsutanol A induces autophagical cell death in human 
      hepatocellular carcinoma cells by increasing reactive oxygen species.
PG  - 655-60
AB  - BACKGROUND AND OBJECTIVE: Hirsutanol A is a novel sesquiterpene compound purified 
      from fungus chondrostereum sp in Sarcophyton tortuosum. Its pharmacologic effect 
      has not been reported yet. This study aimed to investigate cytotoxic effect of 
      Hirsutanol A on hepatocellular carcinoma (HCC) cells and its mechanism. METHODS: 
      Hep3B cells were treated with different concentrations of Hirsutanol A. Cell 
      proliferation was detected by MTT assay. The protein expression of LC3 was 
      determined by Western blot. The generation of reactive oxygen species (ROS) was 
      monitored by flow cytometry. RESULTS: Hirsutanol A significantly inhibited 
      proliferation of Hep3B cells with 50% inhibition concentrations (IC50) of 14.54, 
      6.71, and 3.59 micromol/L when exposed to Hirsutanol A for 24, 48, and 72 h, 
      respectively. Incubation of Hep3B cells with Hirsutanol A markedly increased the 
      level of ROS and the autophagy marker MAP-LC3 conversion from type I to type II. 
      Pre-incubation with an antioxidant N-acetyl cystein (NAC) decreased the level of 
      ROS, and reduced MAP-LC3 I-II conversion, and suppressed cell death. Blocking 
      autophagy with a specific autophagy inhibitor 3-methyladenine (3-MA), the 
      cytotoxic effect of this compound was attenuated. CONCLUSION: Hirsutanol A has 
      potent cytotoxic effect, and can induce autophagic cell death via increasing ROS 
      production.
FAU - Yang, Fen
AU  - Yang F
AD  - State Key Laboratory of Oncology in South China, Guangzhou, Guangdong 510060, PR 
      China.
FAU - Gao, You-Heng
AU  - Gao YH
FAU - Wu, Ke-Wei
AU  - Wu KW
FAU - Deng, Rong
AU  - Deng R
FAU - Li, Dan-Dan
AU  - Li DD
FAU - Wei, Zhi-Xiong
AU  - Wei ZX
FAU - Jiang, Shan
AU  - Jiang S
FAU - Wu, Xiao-Qi
AU  - Wu XQ
FAU - Feng, Gong-Kan
AU  - Feng GK
FAU - Li, Hou-Jin
AU  - Li HJ
FAU - Zhu, Xiao-Feng
AU  - Zhu XF
LA  - eng
PT  - Journal Article
PL  - England
TA  - Chin J Cancer
JT  - Chinese journal of cancer
JID - 101498232
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Free Radical Scavengers)
RN  - 0 (MAP1LC3A protein, human)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Sesquiterpenes)
RN  - 0 (hirsutanol A)
RN  - 5142-23-4 (3-methyladenine)
RN  - JAC85A2161 (Adenine)
RN  - WYQ7N0BPYC (Acetylcysteine)
SB  - IM
MH  - Acetylcysteine/pharmacology
MH  - Adenine/analogs & derivatives/pharmacology
MH  - Agaricales/chemistry
MH  - Antineoplastic Agents/administration & dosage/isolation & 
      purification/pharmacology
MH  - Autophagy/*drug effects
MH  - *Carcinoma, Hepatocellular/metabolism/pathology
MH  - Cell Death/*drug effects
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Dose-Response Relationship, Drug
MH  - Free Radical Scavengers/pharmacology
MH  - Humans
MH  - *Liver Neoplasms/metabolism/pathology
MH  - Microtubule-Associated Proteins/metabolism
MH  - Reactive Oxygen Species/*metabolism
MH  - Sesquiterpenes/administration & dosage/isolation & purification/*pharmacology
EDAT- 2010/07/02 06:00
MHDA- 2012/01/10 06:00
CRDT- 2010/07/02 06:00
PHST- 2010/07/02 06:00 [entrez]
PHST- 2010/07/02 06:00 [pubmed]
PHST- 2012/01/10 06:00 [medline]
AID - 1944-446X201007655 [pii]
AID - 10.5732/cjc.009.10702 [doi]
PST - ppublish
SO  - Chin J Cancer. 2010 Jul;29(7):655-60. doi: 10.5732/cjc.009.10702.