PMID- 20592461
OWN - NLM
STAT- MEDLINE
DCOM- 20100927
LR  - 20191210
IS  - 1881-7823 (Electronic)
IS  - 1881-7815 (Linking)
VI  - 4
IP  - 3
DP  - 2010 Jun
TI  - Isolation of mesenchymal stem cells from bone marrow wastes of spinal fusion 
      procedure (TLIF) for low back pain patients and preparation of bone dusts for 
      transplantable autologous bone graft with a serum glue.
PG  - 110-8
AB  - Low back pain and subsequent disabilities are common. A lumbar spinal fusion 
      procedure is an effective treatment with autologous bone grafts, but harvesting 
      the bone from the iliac crest is associated with risks of complications. New 
      treatments using stem cells together with osteoconductive and otesoinductive 
      materials have made the procedure safer, but the inconsistency of the amount of 
      stem cells harvested from bone marrow aspirate still remains to be solved. This 
      study reports that the bone dusts, usually discarded as surgical wastes during 
      transforaminal lumbar interbody fusion procedure (TLIF procedure), yielded cells 
      which had the characteristics of mesenchymal stem cells (MSCs) in vitro. The 
      cells were positive for the MSC markers and were able to differentiate in 
      osteogenic and adipogenic directions. The cells grew robustly in an 
      osteoconductive material, Bolheal (serum glue), and also proliferated well in 
      culture medium supplemented with autologous serum. Therefore, the bone dust is a 
      good candidate for the alternative source of stem cells other than bone marrow 
      aspirate to increase the safety of the TLIF procedure.
FAU - Ichiyanagi, T
AU  - Ichiyanagi T
AD  - National Institute of Genetics, Mishima, Shizuoka, Japan.
FAU - Anabuki, K
AU  - Anabuki K
FAU - Nishijima, Y
AU  - Nishijima Y
FAU - Ono, H
AU  - Ono H
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Biosci Trends
JT  - Bioscience trends
JID - 101502754
RN  - 0 (Biomarkers)
RN  - 0 (Bone Substitutes)
RN  - 0 (Tissue Adhesives)
RN  - 9001-31-4 (Fibrin)
SB  - IM
MH  - Biomarkers/metabolism
MH  - Bone Marrow Cells/*cytology
MH  - Bone Substitutes/*metabolism
MH  - Bone Transplantation/*methods
MH  - Cell Differentiation
MH  - Cell Separation/methods
MH  - Cells, Cultured
MH  - Female
MH  - Fibrin/metabolism
MH  - Humans
MH  - Immunophenotyping
MH  - Low Back Pain/surgery
MH  - Male
MH  - Mesenchymal Stem Cells/*cytology/physiology
MH  - Spinal Fusion/instrumentation/*methods
MH  - Tissue Adhesives/chemistry/*metabolism
MH  - Transplantation, Autologous/*methods
EDAT- 2010/07/02 06:00
MHDA- 2010/09/29 06:00
CRDT- 2010/07/02 06:00
PHST- 2010/07/02 06:00 [entrez]
PHST- 2010/07/02 06:00 [pubmed]
PHST- 2010/09/29 06:00 [medline]
AID - 311 [pii]
PST - ppublish
SO  - Biosci Trends. 2010 Jun;4(3):110-8.