PMID- 20594272
OWN - NLM
STAT- MEDLINE
DCOM- 20101006
LR  - 20100702
IS  - 1440-1827 (Electronic)
IS  - 1320-5463 (Linking)
VI  - 60
IP  - 7
DP  - 2010 Jul
TI  - Evaluation of HER2 gene amplification in invasive breast cancer using a 
      dual-color chromogenic in situ hybridization (dual CISH).
PG  - 510-5
LID - 10.1111/j.1440-1827.2010.02553.x [doi]
AB  - Fluorescence in situ hybridization (FISH) assay is considered the 'gold standard' 
      for evaluation of HER2/neu (HER2) gene status, however, it is difficult to 
      recognize morphologic features of tumors using fluorescence microscopy. Thus, 
      chromogenic in situ hybridization (CISH) has been proposed as an alternative 
      method to evaluate HER2 gene amplification. Here, we examined the dual color CISH 
      (dual CISH) method which provides information regarding the copy number of the 
      HER2 gene and chromosome 17 centromere from a single slide. We examined 40 cases 
      of invasive ductal carcinomas of the breast that were resected surgically. HER2 
      gene status was assessed with FISH (Abbott) and dual CISH (Dako). HER2 gene 
      amplification status was classified according to the guidelines of the American 
      Society of Clinical Oncology and College of American Pathologists (ASCO/CAP). 
      Comparison of the cut-off values for HER2/chromosome 17 centromere copy number 
      ratio obtained by dual CISH and FISH showed that there was almost perfect 
      agreement between two methods (Kappa coefficient 0.96). The results of the two 
      commercial products were almost consistent for evaluation of HER2 gene counts on 
      the sections. The current study proved that dual CISH is comparable with FISH for 
      evaluating HER2 gene status.
FAU - Kato, Nobuaki
AU  - Kato N
AD  - Division of Diagnostic Pathology, Tokai University Hospital, 143 Shimokasuya, 
      Isehara, Kanagawa, Japan. n-kato@is.icc.u-tokai.ac.jp
FAU - Itoh, Hitoshi
AU  - Itoh H
FAU - Serizawa, Akihiko
AU  - Serizawa A
FAU - Hatanaka, Yutaka
AU  - Hatanaka Y
FAU - Umemura, Shinobu
AU  - Umemura S
FAU - Osamura, R Yoshiyuki
AU  - Osamura RY
LA  - eng
PT  - Journal Article
PL  - Australia
TA  - Pathol Int
JT  - Pathology international
JID - 9431380
SB  - IM
MH  - Breast Neoplasms/*genetics
MH  - Carcinoma, Ductal, Breast/*genetics
MH  - Female
MH  - Gene Amplification
MH  - Genes, erbB-2/*genetics
MH  - Humans
MH  - In Situ Hybridization/*methods
EDAT- 2010/07/03 06:00
MHDA- 2010/10/07 06:00
CRDT- 2010/07/03 06:00
PHST- 2010/07/03 06:00 [entrez]
PHST- 2010/07/03 06:00 [pubmed]
PHST- 2010/10/07 06:00 [medline]
AID - PIN2553 [pii]
AID - 10.1111/j.1440-1827.2010.02553.x [doi]
PST - ppublish
SO  - Pathol Int. 2010 Jul;60(7):510-5. doi: 10.1111/j.1440-1827.2010.02553.x.