PMID- 20599032
OWN - NLM
STAT- MEDLINE
DCOM- 20101004
LR  - 20171116
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 673
IP  - 2
DP  - 2010 Jul 19
TI  - Rapid detection of nivalenol and deoxynivalenol in wheat using surface plasmon 
      resonance immunoassay.
PG  - 173-8
LID - 10.1016/j.aca.2010.05.028 [doi]
AB  - A surface plasmon resonance (SPR) immunoassay using a monoclonal antibody was 
      developed to measure nivalenol (NIV) and deoxynivalenol (DON) contamination in 
      wheat. A highly sensitive and stable DON-immobilized sensor chip was prepared, 
      and an SPR detection procedure was developed. The competitive inhibition assay 
      used a monoclonal antibody that cross-reacts with NIV and DON. The half maximal 
      inhibitory concentration (IC(50)) values of the SPR assay were 28.8 and 14.9 ng 
      mL(-1) for NIV and DON, respectively. The combined responses of NIV and DON in 
      wheat were obtained using a simultaneous detection assay in a one-step cleanup 
      procedure. NIV and DON were separated using a commercial DON-specific 
      immunoaffinity column (IAC) and their responses were obtained using an 
      independent detection assay. Spiked tests using these toxins revealed that 
      recoveries were in the range 91.5-107% with good relative standard deviations 
      (RSDs) (0.40-4.1%) and that detection limits were 0.1 and 0.05 mg kg(-1) for NIV 
      and DON, respectively. The independent detection using IAC showed detection 
      limits of 0.2 and 0.1 mg kg(-1) for NIV and DON, respectively. SPR analysis 
      results were correlated with those obtained using a conventional LC/MS/MS method 
      for wheat co-contaminated with NIV and DON. These results suggested that the 
      developed SPR assay is a practical method to rapidly screen the NIV and DON 
      co-contamination of wheat and one of a very few immunoassays to detect NIV 
      directly.
CI  - 2010 Elsevier B.V. All rights reserved.
FAU - Kadota, Tomoyuki
AU  - Kadota T
AD  - Central Laboratories for Frontier Technology, Center for Food Safety Science, 
      Kirin Holdings Company, Limited, 3 Miyahara, Takasaki, Gunma 370-1295, Japan. 
      Tomoyuki Kadota@kirin.co.jp
FAU - Takezawa, Yoko
AU  - Takezawa Y
FAU - Hirano, Satoshi
AU  - Hirano S
FAU - Tajima, Osamu
AU  - Tajima O
FAU - Maragos, Chris M
AU  - Maragos CM
FAU - Nakajima, Takashi
AU  - Nakajima T
FAU - Tanaka, Toshitsugu
AU  - Tanaka T
FAU - Kamata, Yoichi
AU  - Kamata Y
FAU - Sugita-Konishi, Yoshiko
AU  - Sugita-Konishi Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100525
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Trichothecenes)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 5WOP02RM1U (nivalenol)
RN  - JT37HYP23V (deoxynivalenol)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Cattle
MH  - Immunoassay/*methods
MH  - Serum Albumin, Bovine/chemistry
MH  - Surface Plasmon Resonance/*methods
MH  - Trichothecenes/*analysis
MH  - Triticum/chemistry
EDAT- 2010/07/06 06:00
MHDA- 2010/10/05 06:00
CRDT- 2010/07/06 06:00
PHST- 2010/02/05 00:00 [received]
PHST- 2010/04/09 00:00 [revised]
PHST- 2010/05/18 00:00 [accepted]
PHST- 2010/07/06 06:00 [entrez]
PHST- 2010/07/06 06:00 [pubmed]
PHST- 2010/10/05 06:00 [medline]
AID - S0003-2670(10)00645-8 [pii]
AID - 10.1016/j.aca.2010.05.028 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2010 Jul 19;673(2):173-8. doi: 10.1016/j.aca.2010.05.028. Epub 
      2010 May 25.