PMID- 20599331
OWN - NLM
STAT- MEDLINE
DCOM- 20110506
LR  - 20181201
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 147
IP  - 1-2
DP  - 2011 Jan 10
TI  - Identification of beta-lactamases in human and bovine isolates of Staphylococcus 
      aureus strains having borderline resistance to penicillinase-resistant 
      penicillins (PRPs) with proteomic methods.
PG  - 96-102
LID - 10.1016/j.vetmic.2010.06.006 [doi]
AB  - Methicillin and oxacillin-hydrolyzing enzymes of 6 borderline 
      methicillin-resistant and 1 methicillin-resistant Staphylococcus aureus strains 
      isolated from human clinical samples and 4 borderline methicillin-resistant S. 
      aureus strains isolated from bovine mastitis were investigated. As previous 
      studies suggested the involvement of an additional enzyme besides the 
      penicillinase BlaZ in the determination of borderline resistance, we analyzed the 
      expressed extracellular and membrane-bound beta-lactamases with 2-D gel 
      electrophoresis and mass spectrometry. Our analysis showed that the 
      penicillin-hydrolyzing BlaZ alone was responsible for the hydrolysis of both 
      methicillin and oxacillin. All supernatant and membrane fractions contained the 
      same enzyme with slight sequence variations. The size and pI of the proteins were 
      also variable, probably due to spontaneous hydrolysis and/or posttranslational 
      modifications. Interestingly, we found also cytotoxins and other virulence 
      factors in some nitrocefin-hydrolyzing dots, suggesting that those proteins might 
      have a role in the reduction of local antibiotic concentration.
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Keseru, Judit Szilvia
AU  - Keseru JS
AD  - Department of Human Genetics, University of Debrecen, Debrecen, Hungary. 
      kevia@freemail.hu
FAU - Szabo, Istvan
AU  - Szabo I
FAU - Gal, Zsuzsanna
AU  - Gal Z
FAU - Massidda, Orietta
AU  - Massidda O
FAU - Mingoia, Marina
AU  - Mingoia M
FAU - Kaszanyitzky, Eva
AU  - Kaszanyitzky E
FAU - Janosi, Szilard
AU  - Janosi S
FAU - Hulvely, Julianna
AU  - Hulvely J
FAU - Csorba, Attila
AU  - Csorba A
FAU - Buzas, Krisztina
AU  - Buzas K
FAU - Hunyadi-Gulyas, Eva
AU  - Hunyadi-Gulyas E
FAU - Medzihradszky, Katalin F
AU  - Medzihradszky KF
FAU - Biro, Sandor
AU  - Biro S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100618
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Cephalosporins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Penicillins)
RN  - EC 3.5.2.- (Penicillinase)
RN  - EC 3.5.2.6 (beta-Lactamases)
RN  - EWP54G0J8F (nitrocefin)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/pharmacology
MH  - Cattle
MH  - Cephalosporins/metabolism
MH  - Drug Resistance, Bacterial
MH  - Female
MH  - Humans
MH  - Membrane Proteins/metabolism
MH  - Methicillin-Resistant Staphylococcus aureus/enzymology/isolation & purification
MH  - Penicillinase/metabolism
MH  - Penicillins/*pharmacology
MH  - *Proteomics
MH  - Staphylococcal Infections/microbiology
MH  - Staphylococcus aureus/drug effects/*enzymology/*isolation & purification
MH  - beta-Lactamases/*isolation & purification/*metabolism
EDAT- 2010/07/06 06:00
MHDA- 2011/05/07 06:00
CRDT- 2010/07/06 06:00
PHST- 2010/02/12 00:00 [received]
PHST- 2010/06/08 00:00 [revised]
PHST- 2010/06/09 00:00 [accepted]
PHST- 2010/07/06 06:00 [entrez]
PHST- 2010/07/06 06:00 [pubmed]
PHST- 2011/05/07 06:00 [medline]
AID - S0378-1135(10)00308-1 [pii]
AID - 10.1016/j.vetmic.2010.06.006 [doi]
PST - ppublish
SO  - Vet Microbiol. 2011 Jan 10;147(1-2):96-102. doi: 10.1016/j.vetmic.2010.06.006. 
      Epub 2010 Jun 18.