PMID- 20599681
OWN - NLM
STAT- MEDLINE
DCOM- 20100930
LR  - 20161126
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1797
IP  - 9
DP  - 2010 Sep
TI  - Mitochondrial respiration and membrane potential are regulated by the allosteric 
      ATP-inhibition of cytochrome c oxidase.
PG  - 1672-80
LID - 10.1016/j.bbabio.2010.06.005 [doi]
AB  - This paper describes the problems of measuring the allosteric ATP-inhibition of 
      cytochrome c oxidase (CcO) in isolated mitochondria. Only by using the 
      ATP-regenerating system phosphoenolpyruvate and pyruvate kinase full 
      ATP-inhibition of CcO could be demonstrated by kinetic measurements. The 
      mechanism was proposed to keep the mitochondrial membrane potential (DeltaPsi(m)) 
      in living cells and tissues at low values (100-140 mV), when the matrix ATP/ADP 
      ratios are high. In contrast, high DeltaPsi(m) values (180-220 mV) are generally 
      measured in isolated mitochondria. By using a tetraphenyl phosphonium electrode 
      we observed in isolated rat liver mitochondria with glutamate plus malate as 
      substrates a reversible decrease of DeltaPsi(m) from 233 to 123 mV after addition 
      of phosphoenolpyruvate and pyruvate kinase. The decrease of DeltaPsi(m) is 
      explained by reversal of the gluconeogenetic enzymes pyruvate carboxylase and 
      phosphoenolpyruvate carboxykinase yielding ATP and GTP, thus increasing the 
      matrix ATP/ADP ratio. With rat heart mitochondria, which lack these enzymes, no 
      decrease of DeltaPsi(m) was found. From the data we conclude that high matrix 
      ATP/ADP ratios keep DeltaPsi(m) at low values by the allosteric ATP-inhibition of 
      CcO, thus preventing the generation of reactive oxygen species which could 
      generate degenerative diseases. It is proposed that respiration in living 
      eukaryotic organisms is normally controlled by the DeltaPsi(m)-independent 
      "allosteric ATP-inhibition of CcO." Only when the allosteric ATP-inhibition is 
      switched off under stress, respiration is regulated by "respiratory control," 
      based on DeltaPsi(m) according to the Mitchell Theory.
CI  - 2010 Elsevier B.V. All rights reserved.
FAU - Ramzan, Rabia
AU  - Ramzan R
AD  - Biomedical Research Center, Cardiovascular Laboratory, Philipps-University, 
      D-35032 Marburg, Germany.
FAU - Staniek, Katrin
AU  - Staniek K
FAU - Kadenbach, Bernhard
AU  - Kadenbach B
FAU - Vogt, Sebastian
AU  - Vogt S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100622
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 61D2G4IYVH (Adenosine Diphosphate)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
SB  - IM
MH  - Adenosine Diphosphate/pharmacology
MH  - Adenosine Triphosphate/metabolism/*pharmacology
MH  - Animals
MH  - *Cell Respiration
MH  - Electron Transport Complex IV/*antagonists & inhibitors
MH  - *Membrane Potential, Mitochondrial
MH  - Mitochondria, Heart/*physiology
MH  - Mitochondria, Liver/*physiology
MH  - Rats
EDAT- 2010/07/06 06:00
MHDA- 2010/10/01 06:00
CRDT- 2010/07/06 06:00
PHST- 2010/04/22 00:00 [received]
PHST- 2010/06/01 00:00 [revised]
PHST- 2010/06/07 00:00 [accepted]
PHST- 2010/07/06 06:00 [entrez]
PHST- 2010/07/06 06:00 [pubmed]
PHST- 2010/10/01 06:00 [medline]
AID - S0005-2728(10)00628-6 [pii]
AID - 10.1016/j.bbabio.2010.06.005 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2010 Sep;1797(9):1672-80. doi: 
      10.1016/j.bbabio.2010.06.005. Epub 2010 Jun 22.