PMID- 20600358
OWN - NLM
STAT- MEDLINE
DCOM- 20101027
LR  - 20171116
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1803
IP  - 9
DP  - 2010 Sep
TI  - Lipid rafts modulate the activation but not the maintenance of store-operated 
      Ca(2+) entry.
PG  - 1083-93
LID - 10.1016/j.bbamcr.2010.06.006 [doi]
AB  - Different studies have reported that proteins involved in Ca(2+) entry are 
      localized in discrete plasma membrane domains known as lipid rafts, which have 
      been suggested to support store-operated Ca(2+) entry by facilitating STIM1 
      clustering in endoplasmic reticulum-plasma membrane junctions as well as the 
      interaction of STIM1 with TRPC1. Here we report that treatment of HEK293 cells 
      with thapsigargin (TG) results in the activation of Ca(2+) entry with two 
      components, an early, La(3+)-sensitive, component and a late component that shows 
      both La(3+)-sensitive and -insensitive constituents. Preincubation with 
      methyl-beta-cyclodextrin (MbetaCD) prevented TG-induced activation of Ca(2+) 
      entry but, in contrast, enhanced this process after its activation. Addition of 
      MbetaCD after store depletion did not modify the La(3+)-sensitive store-operated 
      divalent cation entry but increased La(3+)-insensitive non-capacitative Ca(2+) 
      entry. Cell stimulation with TG results in a transient increase in Orai1 
      co-immunoprecipitation with STIM1, TRPC1 and TRPC6. TG-induced association of 
      these proteins was significantly attenuated by preincubation for 30 min with 
      MbetaCD, without altering surface expression of Orai1 or TRPCs. In contrast, the 
      association of Orai1 with STIM1 or TRPC1 was unaffected when MbetaCD was added 
      after store depletion with TG. Addition of MbetaCD to TG-treated cells promoted 
      dissociation between Orai1 and TRPC6, as well as non-capacitative Ca(2+) entry. 
      TRPC6 expression silencing indicates that MbetaCD-enhanced non-capacitative 
      Ca(2+) entry was mediated by TRPC6. In conclusion, lipid raft domains are 
      necessary for the activation but not the maintenance of SOCE probably due to the 
      support of the formation of Ca(2+) signalling complexes involving Orai1, TRPCs 
      and STIM1.
CI  - Copyright 2010 Elsevier B.V. All rights reserved.
FAU - Galan, Carmen
AU  - Galan C
AD  - Department of Physiology (Cell Physiology Research Group), University of 
      Extremadura, 10071 Caceres, Spain.
FAU - Woodard, Geoffrey E
AU  - Woodard GE
FAU - Dionisio, Natalia
AU  - Dionisio N
FAU - Salido, Gines M
AU  - Salido GM
FAU - Rosado, Juan A
AU  - Rosado JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100622
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Calcium Channels)
RN  - 0 (Cations, Divalent)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Membrane Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (ORAI1 Protein)
RN  - 0 (ORAI1 protein, human)
RN  - 0 (STIM1 protein, human)
RN  - 0 (Stromal Interaction Molecule 1)
RN  - 0 (TRPC Cation Channels)
RN  - 0 (TRPC6 Cation Channel)
RN  - 0 (TRPC6 protein, human)
RN  - 0 (beta-Cyclodextrins)
RN  - 0 (methyl-beta-cyclodextrin)
RN  - 0 (transient receptor potential cation channel, subfamily C, member 1)
RN  - 67526-95-8 (Thapsigargin)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Calcium/*metabolism
MH  - Calcium Channels/drug effects/metabolism/physiology
MH  - *Calcium Signaling/drug effects/physiology
MH  - Cations, Divalent/metabolism
MH  - Cells, Cultured
MH  - Enzyme Inhibitors/pharmacology
MH  - Humans
MH  - Membrane Microdomains/drug effects/*physiology
MH  - Membrane Proteins/metabolism
MH  - Neoplasm Proteins/metabolism
MH  - ORAI1 Protein
MH  - Protein Binding
MH  - Stromal Interaction Molecule 1
MH  - TRPC Cation Channels/metabolism/physiology
MH  - TRPC6 Cation Channel
MH  - Thapsigargin/pharmacology
MH  - beta-Cyclodextrins/pharmacology
EDAT- 2010/07/06 06:00
MHDA- 2010/10/28 06:00
CRDT- 2010/07/06 06:00
PHST- 2010/04/05 00:00 [received]
PHST- 2010/06/02 00:00 [revised]
PHST- 2010/06/11 00:00 [accepted]
PHST- 2010/07/06 06:00 [entrez]
PHST- 2010/07/06 06:00 [pubmed]
PHST- 2010/10/28 06:00 [medline]
AID - S0167-4889(10)00176-X [pii]
AID - 10.1016/j.bbamcr.2010.06.006 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2010 Sep;1803(9):1083-93. doi: 
      10.1016/j.bbamcr.2010.06.006. Epub 2010 Jun 22.