PMID- 20600795
OWN - NLM
STAT- MEDLINE
DCOM- 20101112
LR  - 20100810
IS  - 1096-1208 (Electronic)
IS  - 0882-4010 (Linking)
VI  - 49
IP  - 5
DP  - 2010 Nov
TI  - Staphylococcus aureus induces apoptosis of human monocytic U937 cells via 
      NF-kappaB signaling pathways.
PG  - 252-9
LID - 10.1016/j.micpath.2010.06.007 [doi]
AB  - Staphylococcus aureus (S. aureus) is an opportunistic pathogen and the major 
      causative agent of numerous infections. S. aureus have been shown to induce 
      apoptosis in many cell types. However, the mechanisms regulating primary 
      monocytes and human monocytic U937 cell death following S. aureus challenge are 
      unknown. In this study, we found that infection of primary monocytes and human 
      monocytic U937 cells with S. aureus induced rapid cell death in a dose- and 
      time-dependent manner displaying the characteristic features of apoptosis. 
      Studying the underlying mechanisms we found that the S. aureus-induced apoptosis 
      was associated with a more prominent reduction in expression of the 
      anti-apoptotic protein nuclear transcription factor-kappaB (NF-kappaB). Because 
      expressions of anti-apoptotic Bcl-2, survivin, inhibition of apoptosis protein 
      (cIAP), and X-chromosome-linked inhibitor of apoptosis protein (XIAP) are 
      regulated by NF-kappaB, S. aureus decreased the levels of these proteins in U937 
      cells through inhibition of NF-kappaB. Moreover, S. aureus induced apoptotic 
      genes (Bax and caspase-3) expression, and exposure of primary monocytes and U937 
      cells to S. aureus led to caspase-3 activity. Collectively, these data define a 
      pathway that infection of primary monocytes and U937 cells with S. aureus induces 
      a caspase-dependent inhibition of NF-kappaB.
CI  - Crown Copyright 2010. Published by Elsevier India Pvt Ltd. All rights reserved.
FAU - Wang, Jia He
AU  - Wang JH
AD  - Department of Geriatrics, Shengjing Hospital of China Medical University, 
      Shenyang, PR China. wangjh1@sj-hospital.org
FAU - Zhou, Yi Jun
AU  - Zhou YJ
FAU - He, Ping
AU  - He P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100625
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
RN  - 0 (BAX protein, human)
RN  - 0 (Inhibitor of Apoptosis Proteins)
RN  - 0 (NF-kappa B)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (X-Linked Inhibitor of Apoptosis Protein)
RN  - 0 (XIAP protein, human)
RN  - 0 (bcl-2-Associated X Protein)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - *Apoptosis
MH  - Caspase 3/biosynthesis
MH  - Cells, Cultured
MH  - Down-Regulation
MH  - *Host-Pathogen Interactions
MH  - Humans
MH  - Inhibitor of Apoptosis Proteins/biosynthesis
MH  - Monocytes/*microbiology
MH  - NF-kappa B/*biosynthesis
MH  - Proto-Oncogene Proteins c-bcl-2/biosynthesis
MH  - *Signal Transduction
MH  - Staphylococcus aureus/*pathogenicity
MH  - U937 Cells
MH  - Up-Regulation
MH  - X-Linked Inhibitor of Apoptosis Protein/biosynthesis
MH  - bcl-2-Associated X Protein/biosynthesis
EDAT- 2010/07/06 06:00
MHDA- 2010/11/13 06:00
CRDT- 2010/07/06 06:00
PHST- 2010/03/20 00:00 [received]
PHST- 2010/06/07 00:00 [revised]
PHST- 2010/06/16 00:00 [accepted]
PHST- 2010/07/06 06:00 [entrez]
PHST- 2010/07/06 06:00 [pubmed]
PHST- 2010/11/13 06:00 [medline]
AID - S0882-4010(10)00109-9 [pii]
AID - 10.1016/j.micpath.2010.06.007 [doi]
PST - ppublish
SO  - Microb Pathog. 2010 Nov;49(5):252-9. doi: 10.1016/j.micpath.2010.06.007. Epub 
      2010 Jun 25.