PMID- 20600828
OWN - NLM
STAT- MEDLINE
DCOM- 20110315
LR  - 20131121
IS  - 1873-4596 (Electronic)
IS  - 0891-5849 (Linking)
VI  - 49
IP  - 6
DP  - 2010 Sep 15
TI  - Aberrant repair of etheno-DNA adducts in leukocytes and colon tissue of colon 
      cancer patients.
PG  - 1064-71
LID - 10.1016/j.freeradbiomed.2010.06.027 [doi]
AB  - To assess the role of lipid peroxidation-induced DNA damage and repair in colon 
      carcinogenesis, the excision rates and levels of 1,N(6)-etheno-2'-deoxyadenosine 
      (epsilondA), 3,N(4)-etheno-2'-deoxycytidine (epsilondC), and 
      1,N(2)-etheno-2'-deoxyguanosine (1,N(2)-epsilondG) were analyzed in polymorphic 
      blood leukocytes (PBL) and resected colon tissues of 54 colorectal carcinoma 
      (CRC) patients and PBL of 56 healthy individuals. In PBL the excision rates of 
      1,N(6)-ethenoadenine (epsilonAde) and 3,N(4)-ethenocytosine (epsilonCyt), 
      measured by the nicking of oligodeoxynucleotide duplexes with single lesions, and 
      unexpectedly also the levels of epsilondA and 1,N(2)-epsilondG, measured by 
      LC/MS/MS, were lower in CRC patients than in controls. In contrast the mRNA 
      levels of repair enzymes, alkylpurine- and thymine-DNA glycosylases and abasic 
      site endonuclease (APE1), were higher in PBL of CRC patients than in those of 
      controls, as measured by QPCR. In the target colon tissues epsilonAde and 
      epsilonCyt excision rates were higher, whereas the epsilondA and epsilondC levels 
      in DNA, measured by (32)P-postlabeling, were lower in tumor than in adjacent 
      colon tissue, although a higher mRNA level was observed only for APE1. This 
      suggests that during the onset of carcinogenesis, etheno adduct repair in the 
      colon seems to be under a complex transcriptional and posttranscriptional 
      control, whereby deregulation may act as a driving force for malignancy.
CI  - Copyright 2010 Elsevier Inc. All rights reserved.
FAU - Obtulowicz, Tomasz
AU  - Obtulowicz T
AD  - Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106 
      Warsaw, Poland.
FAU - Winczura, Alicja
AU  - Winczura A
FAU - Speina, Elzbieta
AU  - Speina E
FAU - Swoboda, Maja
AU  - Swoboda M
FAU - Janik, Justyna
AU  - Janik J
FAU - Janowska, Beata
AU  - Janowska B
FAU - Ciesla, Jaroslaw M
AU  - Ciesla JM
FAU - Kowalczyk, Pawel
AU  - Kowalczyk P
FAU - Jawien, Arkadiusz
AU  - Jawien A
FAU - Gackowski, Daniel
AU  - Gackowski D
FAU - Banaszkiewicz, Zbigniew
AU  - Banaszkiewicz Z
FAU - Krasnodebski, Ireneusz
AU  - Krasnodebski I
FAU - Chaber, Andrzej
AU  - Chaber A
FAU - Olinski, Ryszard
AU  - Olinski R
FAU - Nair, Jagadesaan
AU  - Nair J
FAU - Bartsch, Helmut
AU  - Bartsch H
FAU - Douki, Thierry
AU  - Douki T
FAU - Cadet, Jean
AU  - Cadet J
FAU - Tudek, Barbara
AU  - Tudek B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100628
PL  - United States
TA  - Free Radic Biol Med
JT  - Free radical biology & medicine
JID - 8709159
RN  - 0 (1,N(6)-etheno-2'-deoxyadenosine)
RN  - 0 (DNA Adducts)
RN  - 0 (Deoxyadenosines)
RN  - 0W860991D6 (Deoxycytidine)
RN  - 68498-26-0 (3,N(4)-ethenodeoxycytidine)
RN  - EC 3.2.2.- (DNA Glycosylases)
RN  - EC 3.2.2.- (Thymine DNA Glycosylase)
RN  - EC 3.2.2.21 (DNA-3-methyladenine glycosidase II)
RN  - EC 4.2.99.18 (APEX1 protein, human)
RN  - EC 4.2.99.18 (DNA-(Apurinic or Apyrimidinic Site) Lyase)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carcinoma/*genetics/metabolism/pathology/physiopathology
MH  - Case-Control Studies
MH  - Colon/*metabolism/pathology
MH  - Colonic Neoplasms/*genetics/metabolism/pathology/physiopathology
MH  - DNA Adducts/metabolism
MH  - DNA Glycosylases/genetics/*metabolism
MH  - DNA Repair/genetics
MH  - DNA-(Apurinic or Apyrimidinic Site) Lyase/genetics/*metabolism
MH  - Deoxyadenosines/metabolism
MH  - Deoxycytidine/analogs & derivatives/metabolism
MH  - Female
MH  - Humans
MH  - Leukocytes, Mononuclear/*metabolism/pathology
MH  - Lipid Peroxidation
MH  - Middle Aged
MH  - Mutation/genetics
MH  - Thymine DNA Glycosylase/genetics/*metabolism
EDAT- 2010/07/06 06:00
MHDA- 2011/03/16 06:00
CRDT- 2010/07/06 06:00
PHST- 2009/08/07 00:00 [received]
PHST- 2010/06/07 00:00 [revised]
PHST- 2010/06/21 00:00 [accepted]
PHST- 2010/07/06 06:00 [entrez]
PHST- 2010/07/06 06:00 [pubmed]
PHST- 2011/03/16 06:00 [medline]
AID - S0891-5849(10)00392-8 [pii]
AID - 10.1016/j.freeradbiomed.2010.06.027 [doi]
PST - ppublish
SO  - Free Radic Biol Med. 2010 Sep 15;49(6):1064-71. doi: 
      10.1016/j.freeradbiomed.2010.06.027. Epub 2010 Jun 28.