PMID- 20600886
OWN - NLM
STAT- MEDLINE
DCOM- 20110118
LR  - 20101004
IS  - 1873-3441 (Electronic)
IS  - 0939-6411 (Linking)
VI  - 76
IP  - 2
DP  - 2010 Oct
TI  - Enhancement of sustained and controlled protein release using polyelectrolyte 
      complex-loaded injectable and thermosensitive hydrogel.
PG  - 179-88
LID - 10.1016/j.ejpb.2010.06.012 [doi]
AB  - In this study, we aimed at developing controlled and sustained protein release 
      formulations using a combination system of polyelectrolyte complexes (PECs) and 
      thermosensitive poly(organophosphazene) hydrogels as an injectable gel-depot 
      system. In the protein-loaded hydrogel system, the loaded proteins were released 
      rapidly through diffusion regardless of viscosities and mass loss of the gels 
      because of the small hydrodynamic size of the proteins. To suppress protein 
      diffusion and increase protein size, we induced PECs between negatively charged 
      proteins (BSA, gelatin-type B 75 bloom, alpha-amylase, and hGH) and polycations 
      (protamine, polyethylenimine, poly-l-lysine, and poly-l-arginine (PLA)) via an 
      electrostatic interaction and loaded the PECs into the hydrogels. The formations 
      of PECs were affected by molecular weight, pI (or pK(a)), and types of amine 
      group of the used polycations. Unlike other polycations, PLA formed a large 
      uniform complex with BSA, and the PLA/protein complex-loaded hydrogel showed the 
      slowest protein release behavior. In the PEC-loaded hydrogel system, the protein 
      release could also be controlled by viscosities of the gel and weight ratios of 
      polycations and proteins, although the activities of the proteins were decreased 
      in proportion to the PLA amounts. These results suggest that the PEC-loaded 
      injectable and thermosensitive poly(organophosphazene) hydrogel has considerable 
      potential for creating a sustained protein delivery system by using the PEC via 
      electrostatic interaction.
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Park, Mi-Ran
AU  - Park MR
AD  - Life/Health Division, Korea Institute of Science and Technology, Seoul, Republic 
      of Korea.
FAU - Chun, Changju
AU  - Chun C
FAU - Cho, Chong-Su
AU  - Cho CS
FAU - Song, Soo-Chang
AU  - Song SC
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100625
PL  - Netherlands
TA  - Eur J Pharm Biopharm
JT  - European journal of pharmaceutics and biopharmaceutics : official journal of 
      Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
JID - 9109778
RN  - 0 (Cations)
RN  - 0 (Delayed-Action Preparations)
RN  - 0 (Drug Carriers)
RN  - 0 (Electrolytes)
RN  - 0 (Hydrogels)
RN  - 0 (Organophosphorus Compounds)
RN  - 0 (Polymers)
RN  - 0 (Proteins)
RN  - 0 (poly(organophosphazene))
SB  - IM
MH  - Cations/chemistry
MH  - Delayed-Action Preparations
MH  - Drug Carriers/*chemistry
MH  - Electrolytes/chemistry
MH  - Hydrogels
MH  - Molecular Weight
MH  - Organophosphorus Compounds/*chemistry
MH  - Polymers/*chemistry
MH  - Proteins/*administration & dosage/chemistry
MH  - Static Electricity
MH  - Temperature
MH  - Time Factors
MH  - Viscosity
EDAT- 2010/07/06 06:00
MHDA- 2011/01/19 06:00
CRDT- 2010/07/06 06:00
PHST- 2009/11/27 00:00 [received]
PHST- 2010/06/18 00:00 [revised]
PHST- 2010/06/18 00:00 [accepted]
PHST- 2010/07/06 06:00 [entrez]
PHST- 2010/07/06 06:00 [pubmed]
PHST- 2011/01/19 06:00 [medline]
AID - S0939-6411(10)00161-X [pii]
AID - 10.1016/j.ejpb.2010.06.012 [doi]
PST - ppublish
SO  - Eur J Pharm Biopharm. 2010 Oct;76(2):179-88. doi: 10.1016/j.ejpb.2010.06.012. 
      Epub 2010 Jun 25.