PMID- 20601458 OWN - NLM STAT- MEDLINE DCOM- 20101130 LR - 20211020 IS - 1522-1539 (Electronic) IS - 0363-6135 (Print) IS - 0363-6135 (Linking) VI - 299 IP - 4 DP - 2010 Oct TI - High-precision recording of the action potential in isolated cardiomyocytes using the near-infrared fluorescent dye di-4-ANBDQBS. PG - H1271-81 LID - 10.1152/ajpheart.00248.2010 [doi] AB - The use of voltage-sensitive fluorescent dyes (VSD) for noninvasive measurement of the action potential (AP) in isolated cells has been hindered by low-photon yield of the preparation, dye toxicity, and photodynamic damage. Here we used a new red-shifted VSD, di-4-ANBDQBS, and a fast electron-multiplied charge-coupled device camera for optical AP (OAP) recording in guinea pig cardiac myocytes. Loading di-4-ANBDQBS did not alter APs recorded with micropipette. With short laser exposures (just enough to record one OAP every 1-5 min), di-4-ANBDQBS yielded fluorescent signals with very high signal-to-background ratios (change in fluorescence on depolarization/fluorescence at resting potential: 19.2 +/- 4.1%) and signal-to-noise ratios (40 +/- 13.2). Quantum chemical calculations comparing the ANBDQ chromophore to the conventional ANEP chromophore showed that the higher wavelength and the greater voltage sensitivity of the former have the same electro-optical origin: a longer path for electron redistribution in the excited state. OAP closely tracked simultaneously recorded electrical APs, permitting measurement of AP duration within 1% error. Prolonged laser exposure caused progressive AP duration prolongation and instability. However, these effects were alleviated or abolished by reducing the dye concentration and by perfusion with antioxidants. Thus the presented technique provides a unique opportunity for noninvasive AP recording in single cardiomyocytes. FAU - Warren, Mark AU - Warren M AD - Nora Eccles Harrison Cardiovascular Research and Training Institute, University of Utah, Salt Lake City, UT 84112-5000, USA. warren@cvrti.utah.edu FAU - Spitzer, Kenneth W AU - Spitzer KW FAU - Steadman, Bruce W AU - Steadman BW FAU - Rees, Tyler D AU - Rees TD FAU - Venable, Paul AU - Venable P FAU - Taylor, Tyson AU - Taylor T FAU - Shibayama, Junko AU - Shibayama J FAU - Yan, Ping AU - Yan P FAU - Wuskell, Joseph P AU - Wuskell JP FAU - Loew, Leslie M AU - Loew LM FAU - Zaitsev, Alexey V AU - Zaitsev AV LA - eng GR - R01 EB001963/EB/NIBIB NIH HHS/United States GR - 5R37HL042873/HL/NHLBI NIH HHS/United States GR - 5R01HL088444/HL/NHLBI NIH HHS/United States GR - R01 HL088444/HL/NHLBI NIH HHS/United States GR - 2R01EB001963/EB/NIBIB NIH HHS/United States GR - R01 EB001963-27/EB/NIBIB NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20100702 PL - United States TA - Am J Physiol Heart Circ Physiol JT - American journal of physiology. Heart and circulatory physiology JID - 100901228 RN - 0 (1-(4-sulfanatobutyl)-4-(beta-(2-(di-n-butylamino)-6-naphthyl)butadienyl)quinolinium betaine) RN - 0 (Fluorescent Dyes) RN - 0 (Quinolinium Compounds) RN - CKR7XL41N4 (2-Naphthylamine) SB - IM MH - 2-Naphthylamine/*analogs & derivatives MH - Action Potentials/*physiology MH - Animals MH - Electrophysiologic Techniques, Cardiac/methods MH - *Fluorescent Dyes MH - Guinea Pigs MH - Models, Animal MH - Myocytes, Cardiac/cytology/*physiology MH - Patch-Clamp Techniques/*methods MH - *Quinolinium Compounds PMC - PMC2957348 EDAT- 2010/07/06 06:00 MHDA- 2010/12/14 06:00 PMCR- 2011/10/01 CRDT- 2010/07/06 06:00 PHST- 2010/07/06 06:00 [entrez] PHST- 2010/07/06 06:00 [pubmed] PHST- 2010/12/14 06:00 [medline] PHST- 2011/10/01 00:00 [pmc-release] AID - ajpheart.00248.2010 [pii] AID - H-00248-2010 [pii] AID - 10.1152/ajpheart.00248.2010 [doi] PST - ppublish SO - Am J Physiol Heart Circ Physiol. 2010 Oct;299(4):H1271-81. doi: 10.1152/ajpheart.00248.2010. Epub 2010 Jul 2.