PMID- 20601541
OWN - NLM
STAT- MEDLINE
DCOM- 20101122
LR  - 20220321
IS  - 1933-7205 (Electronic)
IS  - 1933-7191 (Print)
IS  - 1933-7191 (Linking)
VI  - 17
IP  - 9
DP  - 2010 Sep
TI  - Reciprocal communication between endometrial stromal cells and macrophages.
PG  - 809-22
LID - 10.1177/1933719110371854 [doi]
AB  - This study tested the hypothesis that reciprocal communication occurs between 
      macrophages and cultured human endometrial stromal cells and that this 
      communication may contribute to the pathology of endometriosis. An endometrial 
      stromal cell line (telomerase-immortalized human endometrial stromal cell 
      [T-HESC]) was treated with macrophage-conditioned medium (CM) +/- estradiol + 
      progesterone. Macrophages were treated without or with T-HESC CM. DNA microarray 
      identified 716 differentially expressed genes in T-HESCs in response to factors 
      secreted by macrophages. Upregulated genes in T-HESC included interleukin 8 
      (IL-8)/chemokine (C-X-C motif) ligand 8 (CXCL8), matrix metalloproteinase 3 
      (MMP3), phospholamban, cysteine-rich angiogenic inducer 61 (CYR61), connective 
      tissue growth factor (CTGF), tenascin C, and nicotinamide N-methyltransferase 
      (NNMT), whereas integrin alpha-6 was downregulated. In contrast, 15 named genes 
      were differentially expressed in macrophages in response to factors secreted by 
      endometrial stromal cells. The data document reciprocal communication between 
      macrophages and endometrial stromal cells and suggest that interaction with 
      macrophages stimulates the expression of genes in endometrial stromal cells that 
      may support the establishment of endometriosis.
FAU - Eyster, Kathleen M
AU  - Eyster KM
AD  - Division of Basic Biomedical Sciences, Sanford School of Medicine of the 
      University of South Dakota, Vermillion, SD 57069, USA. Kathleen.Eyster@usd.edu
FAU - Hansen, Keith A
AU  - Hansen KA
FAU - Winterton, Emily
AU  - Winterton E
FAU - Klinkova, Olga
AU  - Klinkova O
FAU - Drappeau, Donis
AU  - Drappeau D
FAU - Mark-Kappeler, Connie J
AU  - Mark-Kappeler CJ
LA  - eng
GR  - P20 GM103443/GM/NIGMS NIH HHS/United States
GR  - P20 RR016479/RR/NCRR NIH HHS/United States
GR  - 2 P20 RR016479/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20100702
PL  - United States
TA  - Reprod Sci
JT  - Reproductive sciences (Thousand Oaks, Calif.)
JID - 101291249
RN  - 0 (Culture Media, Conditioned)
RN  - 4TI98Z838E (Estradiol)
RN  - 9002-62-4 (Prolactin)
RN  - C2QI4IOI2G (Medroxyprogesterone Acetate)
SB  - IM
MH  - Culture Media, Conditioned/metabolism
MH  - Endometriosis/genetics/*metabolism/pathology
MH  - Endometrium/drug effects/*metabolism/pathology
MH  - Estradiol/pharmacology
MH  - Female
MH  - Gene Expression Profiling/methods
MH  - Gene Expression Regulation
MH  - Humans
MH  - Macrophages/drug effects/*metabolism
MH  - Medroxyprogesterone Acetate/pharmacology
MH  - Oligonucleotide Array Sequence Analysis
MH  - *Paracrine Communication/drug effects/genetics
MH  - Prolactin/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Stromal Cells/drug effects/*metabolism/pathology
MH  - U937 Cells
PMC - PMC4346189
MID - NIHMS665420
EDAT- 2010/07/06 06:00
MHDA- 2010/12/14 06:00
PMCR- 2015/03/02
CRDT- 2010/07/06 06:00
PHST- 2010/07/06 06:00 [entrez]
PHST- 2010/07/06 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
PHST- 2015/03/02 00:00 [pmc-release]
AID - 1933719110371854 [pii]
AID - 10.1177/1933719110371854 [doi]
PST - ppublish
SO  - Reprod Sci. 2010 Sep;17(9):809-22. doi: 10.1177/1933719110371854. Epub 2010 Jul 
      2.