PMID- 20602950
OWN - NLM
STAT- MEDLINE
DCOM- 20101116
LR  - 20141120
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 82
IP  - 2
DP  - 2010 Jul 15
TI  - Using sweeping-micellar electrokinetic chromatography to determine voriconazole 
      in patient plasma.
PG  - 653-9
LID - 10.1016/j.talanta.2010.05.027 [doi]
AB  - Invasive fungal infection is a life-threatening condition; its occurrence has 
      increased significantly over the past 20 years. We have developed a sensitive and 
      efficient sweeping-micellar electrokinetic chromatography (sweeping-MEKC) method 
      to quantify voriconazole, a potent triazole antifungal drug, in patient plasma. 
      Solid phase extraction (SPE) conditions were first optimized to minimize plasma 
      interference while maintaining a high recovery; the sweeping-MEKC conditions were 
      then systematically optimized to obtain a high sweeping efficiency with good 
      selectivity. Under the optimal analytical conditions, voriconazole was 
      baseline-separated from endogenous materials within 10.5 min with a limit of 
      detection of 0.075 microg mL(-1). The background electrolyte comprised 40 mM 
      phosphoric acid, 110 mM sodium dodecyl sulfate, and 20% acetonitrile. In terms of 
      method repeatability, the relative standard deviations (RSDs) of the migration 
      time and the peak area (intra-day; n=6) were both less than 5.5%; in terms of 
      intermediate precision, and the RSDs of the peak area and the migration time 
      (inter-day; n=3) were both less than 6.3%. We successfully applied this developed 
      method to the quantitative determination of plasma voriconazole levels in 16 
      patients; the results correlated well with those obtained through analyses using 
      high-performance liquid chromatography. This sweeping-MEKC method is accurate and 
      efficient and appears to be applicable to therapeutic drug monitoring and 
      clinical research.
CI  - Copyright 2010 Elsevier B.V. All rights reserved.
FAU - Lin, Shu-Chiao
AU  - Lin SC
AD  - School of Pharmacy, College of Medicine, National Taiwan University, Taipei, 
      Taiwan.
FAU - Lin, Shu-Wen
AU  - Lin SW
FAU - Chen, Jhong-Min
AU  - Chen JM
FAU - Kuo, Ching-Hua
AU  - Kuo CH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100516
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Antifungal Agents)
RN  - 0 (Micelles)
RN  - 0 (Pyrimidines)
RN  - 0 (Triazoles)
RN  - JFU09I87TR (Voriconazole)
SB  - IM
MH  - Antifungal Agents/blood
MH  - Chromatography, High Pressure Liquid
MH  - Chromatography, Micellar Electrokinetic Capillary/*methods
MH  - Humans
MH  - Limit of Detection
MH  - *Micelles
MH  - Molecular Structure
MH  - Pyrimidines/*blood
MH  - Triazoles/*blood
MH  - Voriconazole
EDAT- 2010/07/07 06:00
MHDA- 2010/11/17 06:00
CRDT- 2010/07/07 06:00
PHST- 2010/03/19 00:00 [received]
PHST- 2010/05/09 00:00 [revised]
PHST- 2010/05/10 00:00 [accepted]
PHST- 2010/07/07 06:00 [entrez]
PHST- 2010/07/07 06:00 [pubmed]
PHST- 2010/11/17 06:00 [medline]
AID - S0039-9140(10)00367-X [pii]
AID - 10.1016/j.talanta.2010.05.027 [doi]
PST - ppublish
SO  - Talanta. 2010 Jul 15;82(2):653-9. doi: 10.1016/j.talanta.2010.05.027. Epub 2010 
      May 16.