PMID- 20603172
OWN - NLM
STAT- MEDLINE
DCOM- 20110131
LR  - 20211203
IS  - 1873-5169 (Electronic)
IS  - 0196-9781 (Print)
IS  - 0196-9781 (Linking)
VI  - 31
IP  - 10
DP  - 2010 Oct
TI  - Melanocortin-4 receptor activation promotes insulin-stimulated mTOR signaling.
PG  - 1888-93
LID - 10.1016/j.peptides.2010.06.028 [doi]
AB  - The melanocortin signaling system is integral in regulating energy homeostasis. 
      The melanocortin-4 receptor (MC4R) activates several signaling pathways in 
      performance of this function. The effect of MC4R on insulin-stimulated mammalian 
      target of rapamycin (mTOR), a cellular energy sensor, signaling was investigated. 
      The GT1-1 cell line which expresses MC4R expression was utilized. mTOR signaling 
      was measured by Western blotting analysis using Phospho-mTOR (Ser2448) antibody. 
      NDP-MSH dose-dependently enhanced insulin-stimulated mTOR phosphorylation. The 
      MC4R antagonist SHU9119 blocked this effect, demonstrating specificity. The 
      protein kinase A - cyclic AMP pathway and the MAP kinase pathway were not 
      involved in NDP-MSH actions on insulin-stimulated mTOR phosphorylation. In 
      contrast, the AMP-activated protein kinase agonist, AICAR, attenuated this 
      effect. MC4R activation potentiates insulin-stimulated mTOR signaling via the 
      AMPK pathway.
CI  - Copyright (c) 2010 Elsevier Inc. All rights reserved.
FAU - Chai, Biaoxin
AU  - Chai B
AD  - Department of Surgery, University of Michigan, Ann Arbor, MI, USA.
FAU - Li, Ji-Yao
AU  - Li JY
FAU - Zhang, Weizhen
AU  - Zhang W
FAU - Wu, Xiaobin
AU  - Wu X
FAU - Zhang, Chao
AU  - Zhang C
FAU - Mulholland, Michael W
AU  - Mulholland MW
LA  - eng
GR  - R01 DK054032/DK/NIDDK NIH HHS/United States
GR  - R01 DK054032-14/DK/NIDDK NIH HHS/United States
GR  - R37 DK043225/DK/NIDDK NIH HHS/United States
PT  - Journal Article
DEP - 20100731
PL  - United States
TA  - Peptides
JT  - Peptides
JID - 8008690
RN  - 0 (Affinity Labels)
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (Insulin)
RN  - 0 (Receptor, Melanocortin, Type 4)
RN  - 0 (Ribonucleotides)
RN  - 360-97-4 (Aminoimidazole Carboxamide)
RN  - 581-05-5 (alpha-MSH)
RN  - 75921-69-6 (MSH, 4-Nle-7-Phe-alpha-)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - F0X88YW0YK (AICA ribonucleotide)
SB  - IM
MH  - Affinity Labels/metabolism/pharmacology
MH  - Aminoimidazole Carboxamide/analogs & derivatives/metabolism
MH  - Animals
MH  - Cell Line
MH  - Cyclic AMP/metabolism
MH  - Cyclic AMP-Dependent Protein Kinases/metabolism
MH  - Enzyme Activation
MH  - Hypoglycemic Agents/metabolism
MH  - Insulin/*metabolism
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - Receptor, Melanocortin, Type 4/*metabolism
MH  - Ribonucleotides/metabolism
MH  - Signal Transduction/drug effects/*physiology
MH  - TOR Serine-Threonine Kinases/*metabolism
MH  - alpha-MSH/analogs & derivatives/metabolism/pharmacology
PMC - PMC3282553
MID - NIHMS226184
EDAT- 2010/07/07 06:00
MHDA- 2011/02/01 06:00
PMCR- 2012/02/20
CRDT- 2010/07/07 06:00
PHST- 2010/04/30 00:00 [received]
PHST- 2010/06/23 00:00 [revised]
PHST- 2010/06/24 00:00 [accepted]
PHST- 2010/07/07 06:00 [entrez]
PHST- 2010/07/07 06:00 [pubmed]
PHST- 2011/02/01 06:00 [medline]
PHST- 2012/02/20 00:00 [pmc-release]
AID - S0196-9781(10)00272-X [pii]
AID - 10.1016/j.peptides.2010.06.028 [doi]
PST - ppublish
SO  - Peptides. 2010 Oct;31(10):1888-93. doi: 10.1016/j.peptides.2010.06.028. Epub 2010 
      Jul 31.