PMID- 20603193
OWN - NLM
STAT- MEDLINE
DCOM- 20110120
LR  - 20220311
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Print)
IS  - 0306-4522 (Linking)
VI  - 170
IP  - 2
DP  - 2010 Oct 13
TI  - N-methyl-D-aspartate receptor subunit expression in adult and adolescent brain 
      following chronic ethanol exposure.
PG  - 645-54
LID - 10.1016/j.neuroscience.2010.06.065 [doi]
AB  - Substantial evidence suggests that glutamatergic neurotransmission is a critical 
      mediator of the experience-dependent synaptic plasticity that may underlie 
      alcohol dependence. Substance abuse typically begins in adolescence; therefore, 
      the impact of alcohol on glutamatergic systems during this critical time in brain 
      development is of particular importance. The N-methyl-d-aspartate receptor 
      (NMDAR) is involved in developmental mechanisms underlying neuronal 
      differentiation and synaptogenesis and as such may be a target system for alcohol 
      effects during adolescence. In the present study quantitative biochemical 
      determinations were made of the relative abundance of different protein 
      expressions of NMDAR subunits in adolescents and adults after 2 weeks of ethanol 
      vapor exposure, and 24 h and 2 weeks following withdrawal. After 2 weeks of 
      ethanol vapor exposure N-methyl-d-aspartate receptor NR1 subunit (NR1), 
      N-methyl-d-aspartate receptor NR2A subunit (NR2A), and N-methyl-d-aspartate 
      receptor NR2B subunit (NR2B) subunit expression was found to be increased in 
      hippocampus of the adults. In contrast, 2 weeks of ethanol exposure resulted in 
      no significant changes in NR1 and NR2B subunits and a reduction NR2A subunit 
      expression in hippocampus in adolescents. Twenty-four h and 2 weeks following 
      withdrawal from ethanol vapor NR1 and NR2A subunit expression in hippocampus was 
      decreased in adolescents, whereas in adults it had returned to control levels. In 
      frontal cortex, 2 weeks of chronic ethanol exposure produced decreases in NR1 
      subunit expression in both adults and adolescents but also produced decreases in 
      NR2A and NR2B subunit expression in adults that returned or exceeded control 
      levels by 2 weeks following withdrawal from ethanol vapor. These results 
      demonstrate that NMDAR subunit composition can be modulated differentially 
      between adolescents and adults by chronic ethanol exposure and withdrawal. These 
      developmental differences in NMDAR subunits composition may also be associated 
      with the enhanced vulnerability of the adolescent brain to ethanol dependence.
CI  - Copyright 2010 IBRO. Published by Elsevier Ltd. All rights reserved.
FAU - Pian, J P
AU  - Pian JP
AD  - Department of Molecular and Integrative Neurosciences, The Scripps Research 
      Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
FAU - Criado, J R
AU  - Criado JR
FAU - Milner, R
AU  - Milner R
FAU - Ehlers, C L
AU  - Ehlers CL
LA  - eng
GR  - R01 AA014339/AA/NIAAA NIH HHS/United States
GR  - AA014339/AA/NIAAA NIH HHS/United States
GR  - R01 AA006059/AA/NIAAA NIH HHS/United States
GR  - T32 AA007456/AA/NIAAA NIH HHS/United States
GR  - AA006059/AA/NIAAA NIH HHS/United States
GR  - 5T32 AA007456/AA/NIAAA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20100717
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Protein Isoforms)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Administration, Inhalation
MH  - Age Factors
MH  - Animals
MH  - Body Weight/drug effects
MH  - Ethanol/administration & dosage/blood/*pharmacology
MH  - Frontal Lobe/*drug effects/metabolism
MH  - Gene Expression Regulation, Developmental/*drug effects
MH  - Hippocampus/*drug effects/metabolism
MH  - Male
MH  - Protein Isoforms/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, N-Methyl-D-Aspartate/*metabolism
MH  - Substance Withdrawal Syndrome/*metabolism
MH  - Time Factors
PMC - PMC2945397
MID - NIHMS228406
EDAT- 2010/07/07 06:00
MHDA- 2011/01/21 06:00
PMCR- 2011/10/13
CRDT- 2010/07/07 06:00
PHST- 2010/02/04 00:00 [received]
PHST- 2010/06/16 00:00 [revised]
PHST- 2010/06/25 00:00 [accepted]
PHST- 2010/07/07 06:00 [entrez]
PHST- 2010/07/07 06:00 [pubmed]
PHST- 2011/01/21 06:00 [medline]
PHST- 2011/10/13 00:00 [pmc-release]
AID - S0306-4522(10)00937-1 [pii]
AID - 10.1016/j.neuroscience.2010.06.065 [doi]
PST - ppublish
SO  - Neuroscience. 2010 Oct 13;170(2):645-54. doi: 10.1016/j.neuroscience.2010.06.065. 
      Epub 2010 Jul 17.