PMID- 20605047
OWN - NLM
STAT- MEDLINE
DCOM- 20101110
LR  - 20191210
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Linking)
VI  - 31
IP  - 28
DP  - 2010 Oct
TI  - The development of high quality seals for silicon patch-clamp chips.
PG  - 7398-410
LID - 10.1016/j.biomaterials.2010.06.015 [doi]
AB  - Planar patch-clamp is a two-dimensional variation of traditional patch-clamp. By 
      contrast to classical glass micropipette, the seal quality of silicon patch-clamp 
      chips (i.e. seal resistance and seal success rate) have remained poor due to the 
      planar geometry and the nature of the substrate and thus partially obliterate the 
      advantages related to planar patch-clamp. The characterization of physical 
      parameters involved in seal formation is thus of major interest. In this paper, 
      we demonstrate that the physical characterization of surfaces by a set of 
      techniques (Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM), 
      X-ray Photoelectron Spectroscopy (XPS), surface energy (polar and dispersive 
      contributions), drop angles, impedance spectroscopy, combined with a statistical 
      design of experiments (DOE)) allowed us discriminating chips that provide 
      relevant performances for planar patch-clamp analysis. Analyses of seal quality 
      demonstrate that dispersive interactions and micropore size are the most crucial 
      physical parameters of chip surfaces, by contrast to surface roughness and 
      dielectric membrane thickness. This multi-scale study combined with 
      electrophysiological validation of chips on a diverse set of cell-types 
      expressing various ion channels (IRK1, hERG and hNa(v)1.5 channels) unveiled a 
      suitable patch-clamp chip candidate. This original approach may inspire novel 
      strategies for selecting appropriate surface parameters dedicated to biochips.
FAU - Sordel, Thomas
AU  - Sordel T
AD  - CEA, DSV, iRTSV/Biopuces, Grenoble Cedex 9, France.
FAU - Kermarrec, Frederique
AU  - Kermarrec F
FAU - Sinquin, Yann
AU  - Sinquin Y
FAU - Fonteille, Isabelle
AU  - Fonteille I
FAU - Labeau, Michel
AU  - Labeau M
FAU - Sauter-Starace, Fabien
AU  - Sauter-Starace F
FAU - Pudda, Catherine
AU  - Pudda C
FAU - de Crecy, Francois
AU  - de Crecy F
FAU - Chatelain, Francois
AU  - Chatelain F
FAU - De Waard, Michel
AU  - De Waard M
FAU - Arnoult, Christophe
AU  - Arnoult C
FAU - Picollet-D'hahan, Nathalie
AU  - Picollet-D'hahan N
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Ion Channels)
RN  - Z4152N8IUI (Silicon)
SB  - IM
MH  - Animals
MH  - CHO Cells
MH  - Cricetinae
MH  - Cricetulus
MH  - Humans
MH  - Ion Channels/metabolism
MH  - Materials Testing
MH  - *Microelectrodes
MH  - Patch-Clamp Techniques/*instrumentation/*methods
MH  - Silicon/*chemistry
MH  - Surface Properties
EDAT- 2010/07/08 06:00
MHDA- 2010/11/11 06:00
CRDT- 2010/07/08 06:00
PHST- 2010/04/01 00:00 [received]
PHST- 2010/06/04 00:00 [accepted]
PHST- 2010/07/08 06:00 [entrez]
PHST- 2010/07/08 06:00 [pubmed]
PHST- 2010/11/11 06:00 [medline]
AID - S0142-9612(10)00747-7 [pii]
AID - 10.1016/j.biomaterials.2010.06.015 [doi]
PST - ppublish
SO  - Biomaterials. 2010 Oct;31(28):7398-410. doi: 10.1016/j.biomaterials.2010.06.015.