PMID- 2060608
OWN - NLM
STAT- MEDLINE
DCOM- 19910806
LR  - 20190907
IS  - 0014-4800 (Print)
IS  - 0014-4800 (Linking)
VI  - 54
IP  - 3
DP  - 1991 Jun
TI  - Changes of DNA-acridine orange binding in monocytes and endothelial cells of 
      hypertensive arteries.
PG  - 230-41
AB  - The interactions of monocytes and endothelial cells were examined employing 
      acridine orange (AO) binding to chromatin as an electron microscopic probe for 
      studying changes in nuclear activity. The number of AO labeled nuclei were 
      determined in adherent and sub-endothelial monocytes, and endothelial cells 
      either associated with or devoid of monocytes in mesenteric arteries of renal 
      hypertensive and normotensive rats. No AO-positive cell nuceli were found in the 
      few adherent monocytes in the normotensive rats while in hypertensive rats 63% of 
      the adherent monocytes and 86% of the subendothelial monocytes were labeled by 
      AO. The number of AO-positive cell nuclei increased in the endothelium from 2% in 
      normotensive rats to 17% in hypertensive animals. Fifty-seven percent of these 
      endothelial cells were associated with monocytes adhering to their surfaces. 
      Thirty-nine (52%) of AO-positive and 24 (52%) of AO-negative cell pairs 
      represented 84% of cell pairs with identical nuclear activity. These findings 
      indicate a sequential interaction of monocytes and adjacent endothelial cells 
      recognized at the nuclear level. According to other experimental work the binding 
      capacity of chromatin for AO increases as cell nuclei are reactivated by 
      stimulation of cell proliferation. In addition, AO labeling of chromatin 
      visualized electron microscopically is confined to three of the four stages of 
      the cell cycle. Therefore AO labeling in the present experiments might indicate 
      the reentrance of monocytes and endothelial cells into the cell cycle.
FAU - Kerenyi, T
AU  - Kerenyi T
AD  - 2nd Department of Pathology, Semmelweis University, Budapest, Hungary.
FAU - Lehmann, R
AU  - Lehmann R
FAU - Voss, B
AU  - Voss B
FAU - Jellinek, H
AU  - Jellinek H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Exp Mol Pathol
JT  - Experimental and molecular pathology
JID - 0370711
RN  - 9007-49-2 (DNA)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/*metabolism
MH  - Animals
MH  - Cell Adhesion
MH  - DNA/*metabolism
MH  - Endothelium, Vascular/*metabolism
MH  - Hypertension/*metabolism/pathology
MH  - Male
MH  - Microscopy, Electron
MH  - Monocytes/*metabolism/ultrastructure
MH  - Rats
MH  - Rats, Inbred Strains
EDAT- 1991/06/01 00:00
MHDA- 1991/06/01 00:01
CRDT- 1991/06/01 00:00
PHST- 1991/06/01 00:00 [pubmed]
PHST- 1991/06/01 00:01 [medline]
PHST- 1991/06/01 00:00 [entrez]
AID - 0014-4800(91)90033-T [pii]
AID - 10.1016/0014-4800(91)90033-t [doi]
PST - ppublish
SO  - Exp Mol Pathol. 1991 Jun;54(3):230-41. doi: 10.1016/0014-4800(91)90033-t.