PMID- 20607395
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20111110
LR  - 20211020
IS  - 0920-9069 (Print)
IS  - 1573-0778 (Electronic)
IS  - 0920-9069 (Linking)
VI  - 62
IP  - 4
DP  - 2010 Aug
TI  - Inhibitory effects of Lang-du extract on the in vitro and in vivo growth of 
      melanoma cells and its molecular mechanisms of action.
PG  - 357-66
LID - 10.1007/s10616-010-9283-z [doi]
AB  - The purpose of this study is to investigate the effects of Lang-du extract (LDE) 
      from Traditional Chinese Medicine (TCM) Euphorbia fischeriana Steud on the in 
      vitro and in vivo growth of melanoma cells and its molecular mechanisms of 
      action. Our present results have shown that LDE significantly suppressed the in 
      vitro melanoma cell growth in dose- and time-dependent manners. LDE also 
      displayed the synergistic effect with gamma-radiation on the reduction of the cell 
      viability in melanoma cells. The animal experimental results further confirmed 
      that compared with the control group without drug treatment, the tumor volume in 
      mice was significantly and time-dependently less in LDE group. The absolute 
      weight of solid tumor in the LDE group was 7-fold lower than that in the control 
      group. Western blot analysis indicated that LDE markedly down-regulated the 
      expression of anti-apoptotic protein Bcl-2 and up-regulated the level of 
      pro-apoptotic protein Bax, eventually leading the reduction of Bcl-2/Bax protein 
      ratios both in the cultured melanoma cells and in the tumors from 
      melanoma-bearing mice. In addition, LDE significantly reduced the tumor 
      progression-associated protein levels of vascular endothelial growth factor 
      (VEGF), hepatocyte growth factor/scatter factor (HGF/SF), and osteopontin (OPN) 
      in tumors from the LDE-treated mice. Our findings suggest that LDE may have a 
      wide therapeutic and/or adjuvant therapeutic application in the treatment of 
      melanoma and other cancer.
FAU - Wang, Liping
AU  - Wang L
AD  - Laboratory of Molecular Pharmacology, School of Pharmacy, Yantai University, No. 
      30, Qing Quan Lu, Lai Shan Qu, 264005, Yantai, Shandong Province, China.
FAU - Duan, Huiying
AU  - Duan H
FAU - Wang, Yishan
AU  - Wang Y
FAU - Liu, Kun
AU  - Liu K
FAU - Jiang, Peng
AU  - Jiang P
FAU - Qu, Zhen
AU  - Qu Z
FAU - Yagasaki, Kazumi
AU  - Yagasaki K
FAU - Zhang, Guoying
AU  - Zhang G
LA  - eng
PT  - Journal Article
DEP - 20100704
PL  - United States
TA  - Cytotechnology
JT  - Cytotechnology
JID - 8807027
PMC - PMC2978309
EDAT- 2010/07/08 06:00
MHDA- 2010/07/08 06:01
PMCR- 2011/08/01
CRDT- 2010/07/08 06:00
PHST- 2010/02/13 00:00 [received]
PHST- 2010/06/04 00:00 [accepted]
PHST- 2010/07/08 06:00 [entrez]
PHST- 2010/07/08 06:00 [pubmed]
PHST- 2010/07/08 06:01 [medline]
PHST- 2011/08/01 00:00 [pmc-release]
AID - 9283 [pii]
AID - 10.1007/s10616-010-9283-z [doi]
PST - ppublish
SO  - Cytotechnology. 2010 Aug;62(4):357-66. doi: 10.1007/s10616-010-9283-z. Epub 2010 
      Jul 4.