PMID- 20607592
OWN - NLM
STAT- MEDLINE
DCOM- 20110310
LR  - 20211020
IS  - 1573-4919 (Electronic)
IS  - 0300-8177 (Linking)
VI  - 344
IP  - 1-2
DP  - 2010 Nov
TI  - Protein kinase Cdelta mediates MCP-1 mRNA stabilization in vascular smooth muscle 
      cells.
PG  - 73-9
LID - 10.1007/s11010-010-0530-6 [doi]
AB  - Monocyte chemoattractant protein-1 (MCP-1) is an inflammatory chemokine that 
      promotes atherosclerosis and is a mediator of the response to arterial injury. We 
      previously demonstrated that platelet-derived growth factor (PDGF) and 
      angiotensin II (Ang) induce the accumulation of MCP-1 mRNA in vascular smooth 
      muscle cells mainly by increasing mRNA stability. In the present study, we have 
      examined the signaling pathways involved in this stabilization of MCP-1 mRNA. The 
      effect of PDGF (BB isoform) and Ang on MCP-1 mRNA stability was mediated by the 
      PDGF beta and angiotensin II receptor AT1R, respectively, and did not involve 
      transactivation between the two receptors. The effect of PDGF-BB was blocked by 
      inhibitors of protein kinase C (PKC), but not by inhibitors of phosphoinositol 
      3-kinase (PI3K), Src, or NADPH oxidase (NADPHox). In contrast, the effect of Ang 
      was blocked by inhibitors of Src, and PKC, but not by inhibitors of PI3 K, or 
      NADPHox. The effect of PDGF BB on MCP-1 mRNA stability was blocked by siRNA 
      directed against PKCdelta and protein kinase D (PKD), whereas the effect of Ang was 
      blocked only by siRNA directed against PKCdelta. These results suggest that the 
      enhancement of MCP-1 mRNA stability by PDGF-BB and Ang are mediated by distinct 
      "proximal" signaling pathways that converge on activation of PKCdelta. This study 
      identifies a novel role for PKCdelta in mediating mRNA stability in smooth muscle 
      cells.
FAU - Liu, Bin
AU  - Liu B
AD  - Department of Medicine, University of Rochester Medical Center, Rochester, NY 
      14642, USA. Bin_liu@urmc.rochester.edu
FAU - Dhawan, Latika
AU  - Dhawan L
FAU - Blaxall, Burns C
AU  - Blaxall BC
FAU - Taubman, Mark B
AU  - Taubman MB
LA  - eng
GR  - P01 HL77789/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20100704
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.11.13 (Protein Kinase C-delta)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Chemokine CCL2/*genetics
MH  - Male
MH  - Muscle, Smooth, Vascular/cytology/*enzymology
MH  - Protein Kinase C-delta/*metabolism
MH  - RNA, Messenger/*genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Signal Transduction
MH  - Transcriptional Activation
EDAT- 2010/07/08 06:00
MHDA- 2011/03/11 06:00
CRDT- 2010/07/08 06:00
PHST- 2010/01/11 00:00 [received]
PHST- 2010/06/22 00:00 [accepted]
PHST- 2010/07/08 06:00 [entrez]
PHST- 2010/07/08 06:00 [pubmed]
PHST- 2011/03/11 06:00 [medline]
AID - 10.1007/s11010-010-0530-6 [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2010 Nov;344(1-2):73-9. doi: 10.1007/s11010-010-0530-6. Epub 
      2010 Jul 4.